Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone
The quinone avarone, isolated from the marine sponge Dysidea avara, possesses the ability to chemically modify proteins. In this work, modification of lectin isolated from the coral Gerardia savaglia by avarone was examined. The techniques used for studying the modification were: SDS PAGE, isoelectr...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2007-12-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.shd.org.yu/JSCS/Vol72/No12/JSCS_V72_No12-11.pdf |
| _version_ | 1811319070268588032 |
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| author | IVANA PAJIC ZORAN VUJCIC MIROSLAVA VUJCIC IRENA NOVAKOVIC DUSAN SLADIC MIROSLAV J. GASIC |
| author_facet | IVANA PAJIC ZORAN VUJCIC MIROSLAVA VUJCIC IRENA NOVAKOVIC DUSAN SLADIC MIROSLAV J. GASIC |
| author_sort | IVANA PAJIC |
| collection | DOAJ |
| description | The quinone avarone, isolated from the marine sponge Dysidea avara, possesses the ability to chemically modify proteins. In this work, modification of lectin isolated from the coral Gerardia savaglia by avarone was examined. The techniques used for studying the modification were: SDS PAGE, isoelectric focusing and hemagglutination testing. The results of the SDS PAGE indicate dimerization of the protein. A shift of the pI toward lower value occurs upon modification. The change of the hemagglutination activity of the protein confirms that chemical modification of G. savaglia lectin by avarone changes its ability to interact with the membrane of erythrocytes. |
| first_indexed | 2024-04-13T12:36:48Z |
| format | Article |
| id | doaj.art-e792ed90389246a8918820183d757ab4 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 |
| language | English |
| last_indexed | 2024-04-13T12:36:48Z |
| publishDate | 2007-12-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-e792ed90389246a8918820183d757ab42022-12-22T02:46:39ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51392007-12-01721212711274Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avaroneIVANA PAJICZORAN VUJCICMIROSLAVA VUJCICIRENA NOVAKOVICDUSAN SLADICMIROSLAV J. GASICThe quinone avarone, isolated from the marine sponge Dysidea avara, possesses the ability to chemically modify proteins. In this work, modification of lectin isolated from the coral Gerardia savaglia by avarone was examined. The techniques used for studying the modification were: SDS PAGE, isoelectric focusing and hemagglutination testing. The results of the SDS PAGE indicate dimerization of the protein. A shift of the pI toward lower value occurs upon modification. The change of the hemagglutination activity of the protein confirms that chemical modification of G. savaglia lectin by avarone changes its ability to interact with the membrane of erythrocytes.http://www.shd.org.yu/JSCS/Vol72/No12/JSCS_V72_No12-11.pdfavaronequinoneGerardia savaglia lectincovalent modification |
| spellingShingle | IVANA PAJIC ZORAN VUJCIC MIROSLAVA VUJCIC IRENA NOVAKOVIC DUSAN SLADIC MIROSLAV J. GASIC Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone Journal of the Serbian Chemical Society avarone quinone Gerardia savaglia lectin covalent modification |
| title | Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone |
| title_full | Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone |
| title_fullStr | Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone |
| title_full_unstemmed | Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone |
| title_short | Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone |
| title_sort | chemical modification of the lectin of the marine coral gerardia savaglia by marine quinone avarone |
| topic | avarone quinone Gerardia savaglia lectin covalent modification |
| url | http://www.shd.org.yu/JSCS/Vol72/No12/JSCS_V72_No12-11.pdf |
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