Characterization of <i>Prunus Necrotic Ringspot Virus</i> and <i>Cherry Virus</i> A Infecting Myrobalan Rootstock
<i>Prunus necrotic ringspot virus</i> (PNRSV) and <i>cherry virus</i> A (CVA) are two viruses that mainly infect plants of the genus <i>Prunus</i>. Full-length sequences of these two viruses, collected in the Czech Republic from <i>Prunus cerasifera</i>...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/8/1723 |
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| author | Karima Ben Mansour Petr Komínek Marcela Komínková Jana Brožová |
| author_facet | Karima Ben Mansour Petr Komínek Marcela Komínková Jana Brožová |
| author_sort | Karima Ben Mansour |
| collection | DOAJ |
| description | <i>Prunus necrotic ringspot virus</i> (PNRSV) and <i>cherry virus</i> A (CVA) are two viruses that mainly infect plants of the genus <i>Prunus</i>. Full-length sequences of these two viruses, collected in the Czech Republic from <i>Prunus cerasifera</i> plants, were obtained via HTS sequencing. Phylogenetic analyses based on the NJ method and Splitstree tools showed that the Czech PNRSV isolate (ON088600-ON088602) is a divergent isolate from other molecular groups, sharing less than 97% pairwise nucleotide identity with members of other groups. The Czech CVA isolate (ON088603) belonged to molecular subgroup III-2, clustered with isolates from non-cherry hosts, and shared the highest pairwise nucleotide identity (99.7%) with an isolate of Australian origin. |
| first_indexed | 2024-03-10T23:31:10Z |
| format | Article |
| id | doaj.art-e793ec3736324408bac49cf2aa896525 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T23:31:10Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e793ec3736324408bac49cf2aa8965252023-11-19T03:20:53ZengMDPI AGViruses1999-49152023-08-01158172310.3390/v15081723Characterization of <i>Prunus Necrotic Ringspot Virus</i> and <i>Cherry Virus</i> A Infecting Myrobalan RootstockKarima Ben Mansour0Petr Komínek1Marcela Komínková2Jana Brožová3Ecology, Diagnostics and Genetic Resources of Agriculturally Important Viruses, Fungi and Phytoplasmas, Crop Research Institute, Drnovská 507, 161 06 Prague, Czech RepublicEcology, Diagnostics and Genetic Resources of Agriculturally Important Viruses, Fungi and Phytoplasmas, Crop Research Institute, Drnovská 507, 161 06 Prague, Czech RepublicEcology, Diagnostics and Genetic Resources of Agriculturally Important Viruses, Fungi and Phytoplasmas, Crop Research Institute, Drnovská 507, 161 06 Prague, Czech RepublicEcology, Diagnostics and Genetic Resources of Agriculturally Important Viruses, Fungi and Phytoplasmas, Crop Research Institute, Drnovská 507, 161 06 Prague, Czech Republic<i>Prunus necrotic ringspot virus</i> (PNRSV) and <i>cherry virus</i> A (CVA) are two viruses that mainly infect plants of the genus <i>Prunus</i>. Full-length sequences of these two viruses, collected in the Czech Republic from <i>Prunus cerasifera</i> plants, were obtained via HTS sequencing. Phylogenetic analyses based on the NJ method and Splitstree tools showed that the Czech PNRSV isolate (ON088600-ON088602) is a divergent isolate from other molecular groups, sharing less than 97% pairwise nucleotide identity with members of other groups. The Czech CVA isolate (ON088603) belonged to molecular subgroup III-2, clustered with isolates from non-cherry hosts, and shared the highest pairwise nucleotide identity (99.7%) with an isolate of Australian origin.https://www.mdpi.com/1999-4915/15/8/1723PNRSVCVAphylogenetic analysisbiological assaysymptoms |
| spellingShingle | Karima Ben Mansour Petr Komínek Marcela Komínková Jana Brožová Characterization of <i>Prunus Necrotic Ringspot Virus</i> and <i>Cherry Virus</i> A Infecting Myrobalan Rootstock Viruses PNRSV CVA phylogenetic analysis biological assay symptoms |
| title | Characterization of <i>Prunus Necrotic Ringspot Virus</i> and <i>Cherry Virus</i> A Infecting Myrobalan Rootstock |
| title_full | Characterization of <i>Prunus Necrotic Ringspot Virus</i> and <i>Cherry Virus</i> A Infecting Myrobalan Rootstock |
| title_fullStr | Characterization of <i>Prunus Necrotic Ringspot Virus</i> and <i>Cherry Virus</i> A Infecting Myrobalan Rootstock |
| title_full_unstemmed | Characterization of <i>Prunus Necrotic Ringspot Virus</i> and <i>Cherry Virus</i> A Infecting Myrobalan Rootstock |
| title_short | Characterization of <i>Prunus Necrotic Ringspot Virus</i> and <i>Cherry Virus</i> A Infecting Myrobalan Rootstock |
| title_sort | characterization of i prunus necrotic ringspot virus i and i cherry virus i a infecting myrobalan rootstock |
| topic | PNRSV CVA phylogenetic analysis biological assay symptoms |
| url | https://www.mdpi.com/1999-4915/15/8/1723 |
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