Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain
Francis Sahngun Nahm,1,2 Sang-Soep Nahm,3 Woong Ki Han,1 Ho Young Gil,4 Eunjoo Choi,1 Pyung Bok Lee1,2 1Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea; 2College of Medicine, Seoul National University, Seoul, South Korea; 3Laboratory...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2019-03-01
|
| Series: | Journal of Pain Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/increased-cerebral-nuclear-factor-kappa-b-in-a-complex-regional-pain-s-peer-reviewed-article-JPR |
| _version_ | 1819205477368594432 |
|---|---|
| author | Nahm FS Nahm SS Han WK Gil HY Choi E Lee PB |
| author_facet | Nahm FS Nahm SS Han WK Gil HY Choi E Lee PB |
| author_sort | Nahm FS |
| collection | DOAJ |
| description | Francis Sahngun Nahm,1,2 Sang-Soep Nahm,3 Woong Ki Han,1 Ho Young Gil,4 Eunjoo Choi,1 Pyung Bok Lee1,2 1Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea; 2College of Medicine, Seoul National University, Seoul, South Korea; 3Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul, South Korea; 4Department of Anesthesiology and Pain Medicine, Ajou University Hospital, Suwon, South Korea Purpose: Complex regional pain syndrome (CRPS) is a rare but refractory pain disorder. Recent advanced information retrieval studies using text-mining and network analysis have suggested nuclear factor kappa B (NFκB) as a possible central mediator of CRPS. The brain is also known to play important roles in CRPS. The aim of this study was to evaluate changes in cerebral NFκB in rats with CRPS. Materials and methods: The chronic post-ischemia perfusion (CPIP) model was used as the CRPS animal model. O-rings were applied to the left hind paws of the rats. The rats were categorized into three groups according to the results of behavioral tests: the CPIP-positive (A) group, the CPIP-negative (B) group, and the control (C) group. Three weeks after the CPIP procedure, the right cerebrums of the animals were harvested to measure NFkB levels using an ELISA. Results: Animals in group A had significantly decreased mechanical pain thresholds (P<0.01) and significantly increased cerebral NFκB when compared to those in groups B and C (P=0.024). Conclusion: This finding indicates that peripheral injury increases cerebral NFκB levels and implies that minor peripheral injury can lead to the activation of pain-related cerebral processes in CRPS. Keywords: cerebrum, complex regional pain syndrome, enzyme-linked immune-sorbent assay, nuclear factor kappa B, pain |
| first_indexed | 2024-12-23T04:52:20Z |
| format | Article |
| id | doaj.art-e794fa70b07d4c08977aad78503611c2 |
| institution | Directory Open Access Journal |
| issn | 1178-7090 |
| language | English |
| last_indexed | 2024-12-23T04:52:20Z |
| publishDate | 2019-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Pain Research |
| spelling | doaj.art-e794fa70b07d4c08977aad78503611c22022-12-21T17:59:26ZengDove Medical PressJournal of Pain Research1178-70902019-03-01Volume 1290991444477Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brainNahm FSNahm SSHan WKGil HYChoi ELee PBFrancis Sahngun Nahm,1,2 Sang-Soep Nahm,3 Woong Ki Han,1 Ho Young Gil,4 Eunjoo Choi,1 Pyung Bok Lee1,2 1Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea; 2College of Medicine, Seoul National University, Seoul, South Korea; 3Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul, South Korea; 4Department of Anesthesiology and Pain Medicine, Ajou University Hospital, Suwon, South Korea Purpose: Complex regional pain syndrome (CRPS) is a rare but refractory pain disorder. Recent advanced information retrieval studies using text-mining and network analysis have suggested nuclear factor kappa B (NFκB) as a possible central mediator of CRPS. The brain is also known to play important roles in CRPS. The aim of this study was to evaluate changes in cerebral NFκB in rats with CRPS. Materials and methods: The chronic post-ischemia perfusion (CPIP) model was used as the CRPS animal model. O-rings were applied to the left hind paws of the rats. The rats were categorized into three groups according to the results of behavioral tests: the CPIP-positive (A) group, the CPIP-negative (B) group, and the control (C) group. Three weeks after the CPIP procedure, the right cerebrums of the animals were harvested to measure NFkB levels using an ELISA. Results: Animals in group A had significantly decreased mechanical pain thresholds (P<0.01) and significantly increased cerebral NFκB when compared to those in groups B and C (P=0.024). Conclusion: This finding indicates that peripheral injury increases cerebral NFκB levels and implies that minor peripheral injury can lead to the activation of pain-related cerebral processes in CRPS. Keywords: cerebrum, complex regional pain syndrome, enzyme-linked immune-sorbent assay, nuclear factor kappa B, painhttps://www.dovepress.com/increased-cerebral-nuclear-factor-kappa-b-in-a-complex-regional-pain-s-peer-reviewed-article-JPRcerebrumcomplex regional pain syndromeenzyme-linked immune-sorbent assaynuclear factor kappa Bpain |
| spellingShingle | Nahm FS Nahm SS Han WK Gil HY Choi E Lee PB Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain Journal of Pain Research cerebrum complex regional pain syndrome enzyme-linked immune-sorbent assay nuclear factor kappa B pain |
| title | Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain |
| title_full | Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain |
| title_fullStr | Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain |
| title_full_unstemmed | Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain |
| title_short | Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain |
| title_sort | increased cerebral nuclear factor kappa b in a complex regional pain syndrome rat model possible relationship between peripheral injury and the brain |
| topic | cerebrum complex regional pain syndrome enzyme-linked immune-sorbent assay nuclear factor kappa B pain |
| url | https://www.dovepress.com/increased-cerebral-nuclear-factor-kappa-b-in-a-complex-regional-pain-s-peer-reviewed-article-JPR |
| work_keys_str_mv | AT nahmfs increasedcerebralnuclearfactorkappabinacomplexregionalpainsyndromeratmodelpossiblerelationshipbetweenperipheralinjuryandthebrain AT nahmss increasedcerebralnuclearfactorkappabinacomplexregionalpainsyndromeratmodelpossiblerelationshipbetweenperipheralinjuryandthebrain AT hanwk increasedcerebralnuclearfactorkappabinacomplexregionalpainsyndromeratmodelpossiblerelationshipbetweenperipheralinjuryandthebrain AT gilhy increasedcerebralnuclearfactorkappabinacomplexregionalpainsyndromeratmodelpossiblerelationshipbetweenperipheralinjuryandthebrain AT choie increasedcerebralnuclearfactorkappabinacomplexregionalpainsyndromeratmodelpossiblerelationshipbetweenperipheralinjuryandthebrain AT leepb increasedcerebralnuclearfactorkappabinacomplexregionalpainsyndromeratmodelpossiblerelationshipbetweenperipheralinjuryandthebrain |