Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids
In this study, we propose a microfluidic organoid-trapping device used to immobilize human intestinal organoids and apply fluidic stimuli to them. The proposed device has a microchannel with a trapping region with wall gaps between the channel walls and the bottom surface, and a constriction to clog...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/12/2082 |
| _version_ | 1797456259115384832 |
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| author | Miki Matsumoto Yuya Morimoto Toshiro Sato Shoji Takeuchi |
| author_facet | Miki Matsumoto Yuya Morimoto Toshiro Sato Shoji Takeuchi |
| author_sort | Miki Matsumoto |
| collection | DOAJ |
| description | In this study, we propose a microfluidic organoid-trapping device used to immobilize human intestinal organoids and apply fluidic stimuli to them. The proposed device has a microchannel with a trapping region with wall gaps between the channel walls and the bottom surface, and a constriction to clog the organoids in the channel. Since the introduced culture medium escapes from the gap, organoids can be cultured without excessive deformation by hydrostatic pressure. Owing to the characteristics of the organoid-trapping device, we succeeded in trapping human intestinal organoids in the channel. Furthermore, to demonstrate the applicability of the device for culturing intestinal organoids, we induced organoid fusion to form large organoids by aligning the organoids in the channel and applying fluidic shear stress to the organoids to regulate their surface structures. Therefore, we believe that organoid-trapping devices will be useful for investigating organoids aligned or loaded with fluidic stimulation. |
| first_indexed | 2024-03-09T16:05:40Z |
| format | Article |
| id | doaj.art-e795c0d0750e4b0b901bc1f12f5e01be |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T16:05:40Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-e795c0d0750e4b0b901bc1f12f5e01be2023-11-24T16:43:56ZengMDPI AGMicromachines2072-666X2022-11-011312208210.3390/mi13122082Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal OrganoidsMiki Matsumoto0Yuya Morimoto1Toshiro Sato2Shoji Takeuchi3Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, JapanDepartment of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, JapanDepartment of Organoid Medicine, Keio University School of Medicine, The University of Tokyo, 35 Shinano-machi, Shinjyuku-ku, Tokyo 160-0016, JapanDepartment of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, JapanIn this study, we propose a microfluidic organoid-trapping device used to immobilize human intestinal organoids and apply fluidic stimuli to them. The proposed device has a microchannel with a trapping region with wall gaps between the channel walls and the bottom surface, and a constriction to clog the organoids in the channel. Since the introduced culture medium escapes from the gap, organoids can be cultured without excessive deformation by hydrostatic pressure. Owing to the characteristics of the organoid-trapping device, we succeeded in trapping human intestinal organoids in the channel. Furthermore, to demonstrate the applicability of the device for culturing intestinal organoids, we induced organoid fusion to form large organoids by aligning the organoids in the channel and applying fluidic shear stress to the organoids to regulate their surface structures. Therefore, we believe that organoid-trapping devices will be useful for investigating organoids aligned or loaded with fluidic stimulation.https://www.mdpi.com/2072-666X/13/12/2082intestinal organoidenteroidmicrofluidicsfusionperfusion |
| spellingShingle | Miki Matsumoto Yuya Morimoto Toshiro Sato Shoji Takeuchi Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids Micromachines intestinal organoid enteroid microfluidics fusion perfusion |
| title | Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids |
| title_full | Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids |
| title_fullStr | Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids |
| title_full_unstemmed | Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids |
| title_short | Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids |
| title_sort | microfluidic device to manipulate 3d human epithelial cell derived intestinal organoids |
| topic | intestinal organoid enteroid microfluidics fusion perfusion |
| url | https://www.mdpi.com/2072-666X/13/12/2082 |
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