| Summary: | Pathogenic microorganisms have been the primary cause of
foodborne disease and food poisoning throughout the world for years. The use of
natural antimicrobial agents in food coating has been effective in regulating
the adverse effects of pathogens in food. Increasing antimicrobial efficacy in
these coatings is one of the current issues of the food industry. In the
present study, the antimicrobial properties of Enteromorpha sp., which is a marine algae, and gelatin film
solution incorporated with Enteromorpha
sp. methanol extract have been investigated. The contents of Enteromorpha sp. methanol extract were
determined by Gas chromatography–mass spectrometry (GCMS). The most important
components in the extract were methyl palmitoleate, neophytadiene, phytol,
methyl linolenate and methyl stearate. The minimum inhibitory concentration (MIC;
the lowest concentration of test material which results in 99.9% inhibition of
growth) of Enteromorpha sp. on Escherichia coli, Staphylococcus aureus and Candida
albicans were found to be between 10.79 mg/mL and 26.86 mg/mL by
spectrophotometric microdilution technique. The antimicrobial effect of
gelatin- Enteromorpha sp. methanolic
extract film solution against the same pathogens was determined by disc
diffusion method. The inhibition zone of gelatin- Enteromorpha sp. film solution was reported between 0.1 and 5.1 mm
against pathogens. After a 24-h incubation, the effectiveness of the film
solution was lower (1.3 mm) when compared to the extract on E. coli (5.1 mm). As a result, this
study clearly showed that Enteromorpha
sp. could be used as antimicrobial food coating agent, especially, in E. coli struggle.
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