Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective
Quenchbody (Q-body) is a unique, reagentless, fluorescent antibody whose fluorescent intensity increases in an antigen-concentration-dependent manner. Q-body-based homogeneous immunoassay is superior to conventional immunoassays as it does not require multiple immobilization, reaction, and washing s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/11/1262 |
| _version_ | 1797460154435764224 |
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| author | Hee-Jin Jeong |
| author_facet | Hee-Jin Jeong |
| author_sort | Hee-Jin Jeong |
| collection | DOAJ |
| description | Quenchbody (Q-body) is a unique, reagentless, fluorescent antibody whose fluorescent intensity increases in an antigen-concentration-dependent manner. Q-body-based homogeneous immunoassay is superior to conventional immunoassays as it does not require multiple immobilization, reaction, and washing steps. In fact, simply mixing the Q-body and the sample containing the antigen enables the detection of the target antigen. To date, various Q-bodies have been developed to detect biomarkers of interest, including haptens, peptides, proteins, and cells. This review sought to describe the principle of Q-body-based immunoassay and the use of Q-body for various immunoassays. In particular, the Q-bodies were classified from a structural perspective to provide useful information for designing Q-bodies with an appropriate objective. |
| first_indexed | 2024-03-09T17:02:03Z |
| format | Article |
| id | doaj.art-e797b3f04cde4084a317b9430dc53668 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T17:02:03Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-e797b3f04cde4084a317b9430dc536682023-11-24T14:29:43ZengMDPI AGBioengineering2306-53542023-10-011011126210.3390/bioengineering10111262Quenchbodies That Enable One-Pot Detection of Antigens: A Structural PerspectiveHee-Jin Jeong0Department of Biological and Chemical Engineering, Hongik University, Sejong-si 30016, Republic of KoreaQuenchbody (Q-body) is a unique, reagentless, fluorescent antibody whose fluorescent intensity increases in an antigen-concentration-dependent manner. Q-body-based homogeneous immunoassay is superior to conventional immunoassays as it does not require multiple immobilization, reaction, and washing steps. In fact, simply mixing the Q-body and the sample containing the antigen enables the detection of the target antigen. To date, various Q-bodies have been developed to detect biomarkers of interest, including haptens, peptides, proteins, and cells. This review sought to describe the principle of Q-body-based immunoassay and the use of Q-body for various immunoassays. In particular, the Q-bodies were classified from a structural perspective to provide useful information for designing Q-bodies with an appropriate objective.https://www.mdpi.com/2306-5354/10/11/1262QuenchbodyimmunoassayFluoroantibodyon-site assayreagentless sensor |
| spellingShingle | Hee-Jin Jeong Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective Bioengineering Quenchbody immunoassay Fluoroantibody on-site assay reagentless sensor |
| title | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
| title_full | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
| title_fullStr | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
| title_full_unstemmed | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
| title_short | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
| title_sort | quenchbodies that enable one pot detection of antigens a structural perspective |
| topic | Quenchbody immunoassay Fluoroantibody on-site assay reagentless sensor |
| url | https://www.mdpi.com/2306-5354/10/11/1262 |
| work_keys_str_mv | AT heejinjeong quenchbodiesthatenableonepotdetectionofantigensastructuralperspective |