TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell
Summary: Inheritance of chromatin-bound proteins theoretically plays a role in the epigenetic transmission of cellular phenotypes. Protein segregation during cell division is however poorly understood. We now describe TrIPP (Tracking the Inheritance Patterns of Proteins): a live cell imaging method...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221000432 |
| _version_ | 1819297763546890240 |
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| author | Morgane Auboiron Pauline Vasseur Saphia Tonazzini Arame Fall Francesc Rubert Castro Iva Sučec Khadija El Koulali Serge Urbach Marta Radman-Livaja |
| author_facet | Morgane Auboiron Pauline Vasseur Saphia Tonazzini Arame Fall Francesc Rubert Castro Iva Sučec Khadija El Koulali Serge Urbach Marta Radman-Livaja |
| author_sort | Morgane Auboiron |
| collection | DOAJ |
| description | Summary: Inheritance of chromatin-bound proteins theoretically plays a role in the epigenetic transmission of cellular phenotypes. Protein segregation during cell division is however poorly understood. We now describe TrIPP (Tracking the Inheritance Patterns of Proteins): a live cell imaging method for tracking maternal proteins during asymmetric cell divisions of budding yeast. Our analysis of the partitioning pattern of a test set of 18 chromatin-associated proteins reveals that abundant and moderately abundant maternal proteins segregate stochastically and symmetrically between the two cells with the exception of Rxt3p, Fpr4p, and Tup1p, which are preferentially retained in the mother. Low abundance proteins also tend to be retained in the mother cell with the exception of Sir2p and the linker histone H1. Our analysis of chromatin protein behavior in single cells reveals potentially general trends such as coupled protein synthesis and decay and a correlation between protein half-lives and cell-cycle duration. |
| first_indexed | 2024-12-24T05:19:10Z |
| format | Article |
| id | doaj.art-e79c2906df3e40cd8dd6f896b72d408f |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-24T05:19:10Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-e79c2906df3e40cd8dd6f896b72d408f2022-12-21T17:13:30ZengElsevieriScience2589-00422021-02-01242102075TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cellMorgane Auboiron0Pauline Vasseur1Saphia Tonazzini2Arame Fall3Francesc Rubert Castro4Iva Sučec5Khadija El Koulali6Serge Urbach7Marta Radman-Livaja8Institut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France; Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, FranceInstitut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, FranceInstitut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France; Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, FranceInstitut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France; Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, FranceInstitut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, FranceInstitut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, FranceUniversité de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France; Functional Proteomics Platform, IGF _ CNRS INSERM, Université de Montpellier, 141 rue de la Cardonille, 34094 Montpellier cedex 5, FranceUniversité de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France; Functional Proteomics Platform, IGF _ CNRS INSERM, Université de Montpellier, 141 rue de la Cardonille, 34094 Montpellier cedex 5, FranceInstitut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France; Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France; Corresponding authorSummary: Inheritance of chromatin-bound proteins theoretically plays a role in the epigenetic transmission of cellular phenotypes. Protein segregation during cell division is however poorly understood. We now describe TrIPP (Tracking the Inheritance Patterns of Proteins): a live cell imaging method for tracking maternal proteins during asymmetric cell divisions of budding yeast. Our analysis of the partitioning pattern of a test set of 18 chromatin-associated proteins reveals that abundant and moderately abundant maternal proteins segregate stochastically and symmetrically between the two cells with the exception of Rxt3p, Fpr4p, and Tup1p, which are preferentially retained in the mother. Low abundance proteins also tend to be retained in the mother cell with the exception of Sir2p and the linker histone H1. Our analysis of chromatin protein behavior in single cells reveals potentially general trends such as coupled protein synthesis and decay and a correlation between protein half-lives and cell-cycle duration.http://www.sciencedirect.com/science/article/pii/S2589004221000432biological sciencesmolecular biologycell biology |
| spellingShingle | Morgane Auboiron Pauline Vasseur Saphia Tonazzini Arame Fall Francesc Rubert Castro Iva Sučec Khadija El Koulali Serge Urbach Marta Radman-Livaja TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell iScience biological sciences molecular biology cell biology |
| title | TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell |
| title_full | TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell |
| title_fullStr | TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell |
| title_full_unstemmed | TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell |
| title_short | TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell |
| title_sort | tripp a method for tracking the inheritance patterns of proteins in living cells reveals retention of tup1p fpr4p and rpd3l in the mother cell |
| topic | biological sciences molecular biology cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221000432 |
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