Molecular detection of theileria annulata infection: an emerging disease of pet dogs in pakistan
Tick-borne maladies of canines are increasing in sub-tropical and tropical areas around the globe. Theileriosis is an emerging issue in canines, especially dogs but the data regarding the disease prevalence in Asian countries is scarce. Therefore, the current study was designed to check the molecula...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2023-01-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2965 |
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| author | Yasir NAWAB Muhammad IJAZ Rana Muhammad AYYUB Arslan AHMED Iqra MUZAMMIL Nauman Zaheer GHUMMAN Muhammad Umar JAVED Adnan ADIL |
| author_facet | Yasir NAWAB Muhammad IJAZ Rana Muhammad AYYUB Arslan AHMED Iqra MUZAMMIL Nauman Zaheer GHUMMAN Muhammad Umar JAVED Adnan ADIL |
| author_sort | Yasir NAWAB |
| collection | DOAJ |
| description | Tick-borne maladies of canines are increasing in sub-tropical and tropical areas around the globe. Theileriosis is an emerging
issue in canines, especially dogs but the data regarding the disease prevalence in Asian countries is scarce. Therefore, the current study
was designed to check the molecular prevalence of Theileria spp. in dogs of Lahore, Pakistan. A total of 102 dog blood samples were
initially screened by microscopy and later on by PCR. The results revealed that 10.78% (11/102) and 6.86% (7/102) of pet dogs were found
positive by PCR and microscopy, respectively. The phylogenetic analysis showed the sequences resemblance up to 98-99% with cytb gene
fragments of Theileria annulata isolates from Iran, India, Turkey, Sudan, China, and Tunisia. Assumed risk factors revealed a significant
(P<0.05) association of house hygiene, tick infestation, and tick prevention applications with the disease dynamics. The infected animals
also showed a significant decrease (P<0.05) in values of red blood cells (RBCs), hemoglobin level, PCV, and platelet count. This is the first
report regarding the molecular evidence of T. annulata infection in dogs of Pakistan. This study will help control the increasing tick-borne
maladies of dog population in Pakistan. |
| first_indexed | 2024-03-13T05:35:00Z |
| format | Article |
| id | doaj.art-e7a4ae40e40f4171952164bb8bf324f5 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T05:35:00Z |
| publishDate | 2023-01-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-e7a4ae40e40f4171952164bb8bf324f52023-06-14T11:44:49ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-01-01291414810.9775/kvfd.2022.284552965Molecular detection of theileria annulata infection: an emerging disease of pet dogs in pakistanYasir NAWAB0Muhammad IJAZ1Rana Muhammad AYYUB2Arslan AHMED3Iqra MUZAMMIL4Nauman Zaheer GHUMMAN5Muhammad Umar JAVED6Adnan ADIL7Department of Veterinary Medicine, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, 54000, Lahore - PAKISTANDepartment of Veterinary Medicine, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, 54000, Lahore - PAKISTANDepartment of Economics and Business Management, University of Veterinary and Animal Sciences, 54000, Lahore - PAKISTANDepartment of Veterinary Medicine, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, 54000, Lahore - PAKISTANDepartment of Veterinary Medicine, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, 54000, Lahore - PAKISTANDepartment of Veterinary Medicine, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, 54000, Lahore - PAKISTANDepartment of Veterinary Medicine, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, 54000, Lahore - PAKISTANDepartment of Veterinary Surgery and Pet Sciences, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, 54000, Lahore - PAKISTANTick-borne maladies of canines are increasing in sub-tropical and tropical areas around the globe. Theileriosis is an emerging issue in canines, especially dogs but the data regarding the disease prevalence in Asian countries is scarce. Therefore, the current study was designed to check the molecular prevalence of Theileria spp. in dogs of Lahore, Pakistan. A total of 102 dog blood samples were initially screened by microscopy and later on by PCR. The results revealed that 10.78% (11/102) and 6.86% (7/102) of pet dogs were found positive by PCR and microscopy, respectively. The phylogenetic analysis showed the sequences resemblance up to 98-99% with cytb gene fragments of Theileria annulata isolates from Iran, India, Turkey, Sudan, China, and Tunisia. Assumed risk factors revealed a significant (P<0.05) association of house hygiene, tick infestation, and tick prevention applications with the disease dynamics. The infected animals also showed a significant decrease (P<0.05) in values of red blood cells (RBCs), hemoglobin level, PCV, and platelet count. This is the first report regarding the molecular evidence of T. annulata infection in dogs of Pakistan. This study will help control the increasing tick-borne maladies of dog population in Pakistan.https://vetdergikafkas.org/pdf.php?id=2965dogphylogenetic analysistheileria annulatarisk factorshematology |
| spellingShingle | Yasir NAWAB Muhammad IJAZ Rana Muhammad AYYUB Arslan AHMED Iqra MUZAMMIL Nauman Zaheer GHUMMAN Muhammad Umar JAVED Adnan ADIL Molecular detection of theileria annulata infection: an emerging disease of pet dogs in pakistan Kafkas Universitesi Veteriner Fakültesi Dergisi dog phylogenetic analysis theileria annulata risk factors hematology |
| title | Molecular detection of theileria annulata infection: an emerging disease of pet dogs in pakistan |
| title_full | Molecular detection of theileria annulata infection: an emerging disease of pet dogs in pakistan |
| title_fullStr | Molecular detection of theileria annulata infection: an emerging disease of pet dogs in pakistan |
| title_full_unstemmed | Molecular detection of theileria annulata infection: an emerging disease of pet dogs in pakistan |
| title_short | Molecular detection of theileria annulata infection: an emerging disease of pet dogs in pakistan |
| title_sort | molecular detection of theileria annulata infection an emerging disease of pet dogs in pakistan |
| topic | dog phylogenetic analysis theileria annulata risk factors hematology |
| url | https://vetdergikafkas.org/pdf.php?id=2965 |
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