In Vitro and Biological Characterization of Dexamethasone Sodium Phosphate Laden pH-Sensitive and Mucoadhesive Hydroxy Propyl β-Cyclodextrin-g-poly(acrylic acid)/Gelatin Semi-Interpenetrating Networks
The current study reports the fabrication and biological evaluation of hydroxy propyl β-cyclodextrin-g-poly(acrylic acid)/gelatin (HP-β-CD-g-poly(AA)/gelatin) semi-interpenetrating networks (semi-IPN) for colonic delivery of dexamethasone sodium phosphate (DSP). The prepared hydrogels showed pH-depe...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2310-2861/8/5/290 |
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| author | Nyla Ajaz Ikram Ullah Khan Muhammad Irfan Syed Haroon Khalid Sajid Asghar Yasir Mehmood Muhammad Asif Usra Ghulam Hussain Yasser Shahzad Shefaat Ullah Shah Muhammad Usman Munir |
| author_facet | Nyla Ajaz Ikram Ullah Khan Muhammad Irfan Syed Haroon Khalid Sajid Asghar Yasir Mehmood Muhammad Asif Usra Ghulam Hussain Yasser Shahzad Shefaat Ullah Shah Muhammad Usman Munir |
| author_sort | Nyla Ajaz |
| collection | DOAJ |
| description | The current study reports the fabrication and biological evaluation of hydroxy propyl β-cyclodextrin-g-poly(acrylic acid)/gelatin (HP-β-CD-g-poly(AA)/gelatin) semi-interpenetrating networks (semi-IPN) for colonic delivery of dexamethasone sodium phosphate (DSP). The prepared hydrogels showed pH-dependent swelling and mucoadhesive properties. The mucoadhesive strength of hydrogels increased with an increasing concentration of gelatin. Based on the swelling and mucoadhesive properties, AG-1 was chosen as the optimized formulation (0.33% <i>w/w</i> of gelatin and 16.66% <i>w/w</i> of AA) for further analysis. FTIR revealed the successful development of a polymeric network without any interaction with DSP. SEM images revealed a slightly rough surface after drug loading. Drug distribution at the molecular level was confirmed by XRD. In vitro drug release assay showed pH-dependent release, i.e., a minute amount of DSP was released at a pH of 1.2 while 90.58% was released over 72 h at pH 7.4. The optimized formulation did not show any toxic effects on a rabbit’s vital organs and was also hemocompatible, thus confirming the biocompatible nature of the hydrogel. Conclusively, the prepared semi-IPN hydrogel possessed the necessary features, which can be exploited for the colonic delivery of DSP. |
| first_indexed | 2024-03-10T03:51:44Z |
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| id | doaj.art-e7a4ea2b7d9d4999979fd792b0babd1a |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T03:51:44Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-e7a4ea2b7d9d4999979fd792b0babd1a2023-11-23T11:07:42ZengMDPI AGGels2310-28612022-05-018529010.3390/gels8050290In Vitro and Biological Characterization of Dexamethasone Sodium Phosphate Laden pH-Sensitive and Mucoadhesive Hydroxy Propyl β-Cyclodextrin-g-poly(acrylic acid)/Gelatin Semi-Interpenetrating NetworksNyla Ajaz0Ikram Ullah Khan1Muhammad Irfan2Syed Haroon Khalid3Sajid Asghar4Yasir Mehmood5Muhammad Asif6Usra7Ghulam Hussain8Yasser Shahzad9Shefaat Ullah Shah10Muhammad Usman Munir11Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanDepartment of Physiology, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Physiology, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmacy, COMSATS University Islamabad, Lahore Campus, Lahore 54700, PakistanSkin/Regenerative Medicine and Drug Delivery Research, GCPS, Faculty of Pharmacy, Gomal University, Dera Ismail Khan 29050, PakistanDepartment of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka 72388, Saudi ArabiaThe current study reports the fabrication and biological evaluation of hydroxy propyl β-cyclodextrin-g-poly(acrylic acid)/gelatin (HP-β-CD-g-poly(AA)/gelatin) semi-interpenetrating networks (semi-IPN) for colonic delivery of dexamethasone sodium phosphate (DSP). The prepared hydrogels showed pH-dependent swelling and mucoadhesive properties. The mucoadhesive strength of hydrogels increased with an increasing concentration of gelatin. Based on the swelling and mucoadhesive properties, AG-1 was chosen as the optimized formulation (0.33% <i>w/w</i> of gelatin and 16.66% <i>w/w</i> of AA) for further analysis. FTIR revealed the successful development of a polymeric network without any interaction with DSP. SEM images revealed a slightly rough surface after drug loading. Drug distribution at the molecular level was confirmed by XRD. In vitro drug release assay showed pH-dependent release, i.e., a minute amount of DSP was released at a pH of 1.2 while 90.58% was released over 72 h at pH 7.4. The optimized formulation did not show any toxic effects on a rabbit’s vital organs and was also hemocompatible, thus confirming the biocompatible nature of the hydrogel. Conclusively, the prepared semi-IPN hydrogel possessed the necessary features, which can be exploited for the colonic delivery of DSP.https://www.mdpi.com/2310-2861/8/5/290semi-interpenetrating networkhydroxy propyl β cyclodextringelatinacrylic aciddexamethasone |
| spellingShingle | Nyla Ajaz Ikram Ullah Khan Muhammad Irfan Syed Haroon Khalid Sajid Asghar Yasir Mehmood Muhammad Asif Usra Ghulam Hussain Yasser Shahzad Shefaat Ullah Shah Muhammad Usman Munir In Vitro and Biological Characterization of Dexamethasone Sodium Phosphate Laden pH-Sensitive and Mucoadhesive Hydroxy Propyl β-Cyclodextrin-g-poly(acrylic acid)/Gelatin Semi-Interpenetrating Networks Gels semi-interpenetrating network hydroxy propyl β cyclodextrin gelatin acrylic acid dexamethasone |
| title | In Vitro and Biological Characterization of Dexamethasone Sodium Phosphate Laden pH-Sensitive and Mucoadhesive Hydroxy Propyl β-Cyclodextrin-g-poly(acrylic acid)/Gelatin Semi-Interpenetrating Networks |
| title_full | In Vitro and Biological Characterization of Dexamethasone Sodium Phosphate Laden pH-Sensitive and Mucoadhesive Hydroxy Propyl β-Cyclodextrin-g-poly(acrylic acid)/Gelatin Semi-Interpenetrating Networks |
| title_fullStr | In Vitro and Biological Characterization of Dexamethasone Sodium Phosphate Laden pH-Sensitive and Mucoadhesive Hydroxy Propyl β-Cyclodextrin-g-poly(acrylic acid)/Gelatin Semi-Interpenetrating Networks |
| title_full_unstemmed | In Vitro and Biological Characterization of Dexamethasone Sodium Phosphate Laden pH-Sensitive and Mucoadhesive Hydroxy Propyl β-Cyclodextrin-g-poly(acrylic acid)/Gelatin Semi-Interpenetrating Networks |
| title_short | In Vitro and Biological Characterization of Dexamethasone Sodium Phosphate Laden pH-Sensitive and Mucoadhesive Hydroxy Propyl β-Cyclodextrin-g-poly(acrylic acid)/Gelatin Semi-Interpenetrating Networks |
| title_sort | in vitro and biological characterization of dexamethasone sodium phosphate laden ph sensitive and mucoadhesive hydroxy propyl β cyclodextrin g poly acrylic acid gelatin semi interpenetrating networks |
| topic | semi-interpenetrating network hydroxy propyl β cyclodextrin gelatin acrylic acid dexamethasone |
| url | https://www.mdpi.com/2310-2861/8/5/290 |
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