Diel Protein Regulation of Marine Picoplanktonic Communities Assessed by Metaproteomics
The diel cycle is of enormous biological importance in that it imposes temporal structure on ecosystem productivity. In the world’s oceans, microorganisms form complex communities that carry out about half of photosynthesis and the bulk of life-sustaining nutrient cycling. How the functioning of mic...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/12/2621 |
| _version_ | 1797502349157072896 |
|---|---|
| author | Augustin Géron Johannes Werner Philippe Lebaron Ruddy Wattiez Sabine Matallana-Surget |
| author_facet | Augustin Géron Johannes Werner Philippe Lebaron Ruddy Wattiez Sabine Matallana-Surget |
| author_sort | Augustin Géron |
| collection | DOAJ |
| description | The diel cycle is of enormous biological importance in that it imposes temporal structure on ecosystem productivity. In the world’s oceans, microorganisms form complex communities that carry out about half of photosynthesis and the bulk of life-sustaining nutrient cycling. How the functioning of microbial communities is impacted by day and night periods in surface seawater remains to be elucidated. In this study, we compared the day and night metaproteomes of the free-living and the particle-attached bacterial fractions from picoplanktonic communities sampled from the northwest Mediterranean Sea surface. Our results showed similar taxonomic distribution of free-living and particle-attached bacterial populations, with <i>Alphaproteobacteria</i>, <i>Gammaproteobacteria</i> and <i>Cyanobacteria</i> being the most active members. Comparison of the day and night metaproteomes revealed that free-living and particle-attached bacteria were more active during the day and the night, respectively. Interestingly, protein diel variations were observed in the photoautotroph <i>Synechococcales</i> and in (photo)-heterotrophic bacteria such as <i>Flavobacteriales, Pelagibacterales</i> and <i>Rhodobacterales</i>. Moreover, our data demonstrated that diel cycle impacts light-dependent processes such as photosynthesis and UV-stress response in <i>Synechococcales</i> and <i>Rhodobacterales</i>, respectively, while the protein regulation from the ubiquitous <i>Pelagibacterales</i> remained stable over time. This study unravels, for the first time, the diel variation in the protein expression of major free-living and particle-attached microbial players at the sea surface, totaling an analysis of eight metaproteomes. |
| first_indexed | 2024-03-10T03:31:49Z |
| format | Article |
| id | doaj.art-e7a531d577cf4db5b4b3d43ffc223805 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:31:49Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e7a531d577cf4db5b4b3d43ffc2238052023-11-23T09:40:45ZengMDPI AGMicroorganisms2076-26072021-12-01912262110.3390/microorganisms9122621Diel Protein Regulation of Marine Picoplanktonic Communities Assessed by MetaproteomicsAugustin Géron0Johannes Werner1Philippe Lebaron2Ruddy Wattiez3Sabine Matallana-Surget4Proteomics and Microbiology Department, University of Mons, 7000 Mons, BelgiumHigh Performance and Cloud Computing Group, Zentrum für Datenverarbeitung (ZDV), Eberhard Karls University of Tübingen, 72074 Tübingen, GermanyUSR3579, Le Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM) de l’Observatoire Océanologique, UPMC University Paris 06, Sorbonne Universités, 66651 Banyuls-sur-Mer, FranceProteomics and Microbiology Department, University of Mons, 7000 Mons, BelgiumDivision of Biological and Environmental Sciences (BES), Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UKThe diel cycle is of enormous biological importance in that it imposes temporal structure on ecosystem productivity. In the world’s oceans, microorganisms form complex communities that carry out about half of photosynthesis and the bulk of life-sustaining nutrient cycling. How the functioning of microbial communities is impacted by day and night periods in surface seawater remains to be elucidated. In this study, we compared the day and night metaproteomes of the free-living and the particle-attached bacterial fractions from picoplanktonic communities sampled from the northwest Mediterranean Sea surface. Our results showed similar taxonomic distribution of free-living and particle-attached bacterial populations, with <i>Alphaproteobacteria</i>, <i>Gammaproteobacteria</i> and <i>Cyanobacteria</i> being the most active members. Comparison of the day and night metaproteomes revealed that free-living and particle-attached bacteria were more active during the day and the night, respectively. Interestingly, protein diel variations were observed in the photoautotroph <i>Synechococcales</i> and in (photo)-heterotrophic bacteria such as <i>Flavobacteriales, Pelagibacterales</i> and <i>Rhodobacterales</i>. Moreover, our data demonstrated that diel cycle impacts light-dependent processes such as photosynthesis and UV-stress response in <i>Synechococcales</i> and <i>Rhodobacterales</i>, respectively, while the protein regulation from the ubiquitous <i>Pelagibacterales</i> remained stable over time. This study unravels, for the first time, the diel variation in the protein expression of major free-living and particle-attached microbial players at the sea surface, totaling an analysis of eight metaproteomes.https://www.mdpi.com/2076-2607/9/12/2621diel cyclepicoplanktonmicrobial communitiesmetaproteomics |
| spellingShingle | Augustin Géron Johannes Werner Philippe Lebaron Ruddy Wattiez Sabine Matallana-Surget Diel Protein Regulation of Marine Picoplanktonic Communities Assessed by Metaproteomics Microorganisms diel cycle picoplankton microbial communities metaproteomics |
| title | Diel Protein Regulation of Marine Picoplanktonic Communities Assessed by Metaproteomics |
| title_full | Diel Protein Regulation of Marine Picoplanktonic Communities Assessed by Metaproteomics |
| title_fullStr | Diel Protein Regulation of Marine Picoplanktonic Communities Assessed by Metaproteomics |
| title_full_unstemmed | Diel Protein Regulation of Marine Picoplanktonic Communities Assessed by Metaproteomics |
| title_short | Diel Protein Regulation of Marine Picoplanktonic Communities Assessed by Metaproteomics |
| title_sort | diel protein regulation of marine picoplanktonic communities assessed by metaproteomics |
| topic | diel cycle picoplankton microbial communities metaproteomics |
| url | https://www.mdpi.com/2076-2607/9/12/2621 |
| work_keys_str_mv | AT augustingeron dielproteinregulationofmarinepicoplanktoniccommunitiesassessedbymetaproteomics AT johanneswerner dielproteinregulationofmarinepicoplanktoniccommunitiesassessedbymetaproteomics AT philippelebaron dielproteinregulationofmarinepicoplanktoniccommunitiesassessedbymetaproteomics AT ruddywattiez dielproteinregulationofmarinepicoplanktoniccommunitiesassessedbymetaproteomics AT sabinematallanasurget dielproteinregulationofmarinepicoplanktoniccommunitiesassessedbymetaproteomics |