Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin Preparations
The objective of the present study was to prepare and characterize low molecular weight chitosan (LMWC) with different molecular weight and degrees of deacetylation (DDA) and to optimize their use in oral insulin nano delivery systems. Water in oil nanosized systems containing LMWC-insulin polyelec...
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MDPI AG
2015-03-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/4/1710 |
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| author | Nidal A. Qinna Qutuba G. Karwi Nawzat Al-Jbour Mayyas A. Al-Remawi Tawfiq M. Alhussainy Khaldoun A. Al-So'ud Mahmoud M. H. Al Omari Adnan A. Badwan |
| author_facet | Nidal A. Qinna Qutuba G. Karwi Nawzat Al-Jbour Mayyas A. Al-Remawi Tawfiq M. Alhussainy Khaldoun A. Al-So'ud Mahmoud M. H. Al Omari Adnan A. Badwan |
| author_sort | Nidal A. Qinna |
| collection | DOAJ |
| description | The objective of the present study was to prepare and characterize low molecular weight chitosan (LMWC) with different molecular weight and degrees of deacetylation (DDA) and to optimize their use in oral insulin nano delivery systems. Water in oil nanosized systems containing LMWC-insulin polyelectrolyte complexes were constructed and their ability to reduce blood glucose was assessed in vivo on diabetic rats. Upon acid depolymerization and testing by viscosity method, three molecular weights of LMWC namely, 1.3, 13 and 18 kDa were obtained. As for the DDA, three LMWCs of 55%, 80% and 100% DDA were prepared and characterized by spectroscopic methods for each molecular weight. The obtained LMWCs showed different morphological and in silico patterns. Following complexation of LMWCs with insulin, different aggregation sizes were obtained. Moreover, the in vivo tested formulations showed different activities of blood glucose reduction. The highest glucose reduction was achieved with 1.3 kDa LMWC of 55% DDA. The current study emphasizes the importance of optimizing the molecular weight along with the DDA of the incorporated LMWC in oral insulin delivery preparations in order to ensure the highest performance of such delivery systems. |
| first_indexed | 2024-04-14T05:28:43Z |
| format | Article |
| id | doaj.art-e7a8c2042d604c2c8071ab54851a394e |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T05:28:43Z |
| publishDate | 2015-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e7a8c2042d604c2c8071ab54851a394e2022-12-22T02:09:53ZengMDPI AGMarine Drugs1660-33972015-03-011341710172510.3390/md13041710md13041710Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin PreparationsNidal A. Qinna0Qutuba G. Karwi1Nawzat Al-Jbour2Mayyas A. Al-Remawi3Tawfiq M. Alhussainy4Khaldoun A. Al-So'ud5Mahmoud M. H. Al Omari6Adnan A. Badwan7Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, P.O. Box 96134, Amman 11196, JordanDepartment of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, P.O. Box 96134, Amman 11196, JordanThe Jordanian Pharmaceutical Manufacturing Company (PLC), Research and Innovation Centre, P.O. Box 94, Naor 11710, JordanCollege of Pharmacy, Taif University, Taif 5700, Saudi ArabiaDepartment of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, P.O. Box 96134, Amman 11196, JordanDepartment of Chemistry, Faculty of Science, Al al-Bayt University, P.O. Box 130040, Mafraq 25113, JordanThe Jordanian Pharmaceutical Manufacturing Company (PLC), Research and Innovation Centre, P.O. Box 94, Naor 11710, JordanThe Jordanian Pharmaceutical Manufacturing Company (PLC), Research and Innovation Centre, P.O. Box 94, Naor 11710, JordanThe objective of the present study was to prepare and characterize low molecular weight chitosan (LMWC) with different molecular weight and degrees of deacetylation (DDA) and to optimize their use in oral insulin nano delivery systems. Water in oil nanosized systems containing LMWC-insulin polyelectrolyte complexes were constructed and their ability to reduce blood glucose was assessed in vivo on diabetic rats. Upon acid depolymerization and testing by viscosity method, three molecular weights of LMWC namely, 1.3, 13 and 18 kDa were obtained. As for the DDA, three LMWCs of 55%, 80% and 100% DDA were prepared and characterized by spectroscopic methods for each molecular weight. The obtained LMWCs showed different morphological and in silico patterns. Following complexation of LMWCs with insulin, different aggregation sizes were obtained. Moreover, the in vivo tested formulations showed different activities of blood glucose reduction. The highest glucose reduction was achieved with 1.3 kDa LMWC of 55% DDA. The current study emphasizes the importance of optimizing the molecular weight along with the DDA of the incorporated LMWC in oral insulin delivery preparations in order to ensure the highest performance of such delivery systems.http://www.mdpi.com/1660-3397/13/4/1710insulinoral deliveryoligochitosanlow molecular weight chitosannanoparticlesspectroscopyglucosediabetic rats |
| spellingShingle | Nidal A. Qinna Qutuba G. Karwi Nawzat Al-Jbour Mayyas A. Al-Remawi Tawfiq M. Alhussainy Khaldoun A. Al-So'ud Mahmoud M. H. Al Omari Adnan A. Badwan Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin Preparations Marine Drugs insulin oral delivery oligochitosan low molecular weight chitosan nanoparticles spectroscopy glucose diabetic rats |
| title | Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin Preparations |
| title_full | Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin Preparations |
| title_fullStr | Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin Preparations |
| title_full_unstemmed | Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin Preparations |
| title_short | Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin Preparations |
| title_sort | influence of molecular weight and degree of deacetylation of low molecular weight chitosan on the bioactivity of oral insulin preparations |
| topic | insulin oral delivery oligochitosan low molecular weight chitosan nanoparticles spectroscopy glucose diabetic rats |
| url | http://www.mdpi.com/1660-3397/13/4/1710 |
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