Salivary, serological, and cellular immune response to the CoronaVac vaccine in health care workers with or without previous COVID-19
Abstract We investigated the anti-SARS-CoV-2 post-vaccine response through serum and salivary antibodies, serum antibody neutralizing activity and cellular immune response in samples from health care workers who were immunized with two doses of an inactivated virus-based vaccine (CoronaVac) who had...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14283-x |
| _version_ | 1811241165776748544 |
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| author | Marina Mazzilli Ortega Laís Teodoro da Silva Érika Donizetti Candido Yingying Zheng Bruna Tiaki Tiyo Arthur Eduardo Fernandes Ferreira Simone Corrêa-Silva Guilherme Pereira Scagion Fabyano Bruno Leal Vanessa Nascimento Chalup Camila Araújo Valério Gabriela Justamante Händel Schmitz Carina Ceneviva Aline Pivetta Corá Alexandre de Almeida Edison Luiz Durigon Danielle Bruna Leal Oliveira Patricia Palmeira Alberto José da Silva Duarte Magda Carneiro-Sampaio Telma Miyuki Oshiro |
| author_facet | Marina Mazzilli Ortega Laís Teodoro da Silva Érika Donizetti Candido Yingying Zheng Bruna Tiaki Tiyo Arthur Eduardo Fernandes Ferreira Simone Corrêa-Silva Guilherme Pereira Scagion Fabyano Bruno Leal Vanessa Nascimento Chalup Camila Araújo Valério Gabriela Justamante Händel Schmitz Carina Ceneviva Aline Pivetta Corá Alexandre de Almeida Edison Luiz Durigon Danielle Bruna Leal Oliveira Patricia Palmeira Alberto José da Silva Duarte Magda Carneiro-Sampaio Telma Miyuki Oshiro |
| author_sort | Marina Mazzilli Ortega |
| collection | DOAJ |
| description | Abstract We investigated the anti-SARS-CoV-2 post-vaccine response through serum and salivary antibodies, serum antibody neutralizing activity and cellular immune response in samples from health care workers who were immunized with two doses of an inactivated virus-based vaccine (CoronaVac) who had or did not have COVID-19 previously. IgA and IgG antibodies directed at the spike protein were analysed in samples of saliva and/or serum by ELISA and/or chemiluminescence assays; the neutralizing activity of serum antibodies against reference strain B, Gamma and Delta SARS-CoV-2 variants were evaluated using a virus neutralization test and SARS-CoV-2 reactive interferon-gamma T-cell were analysed by flow cytometry. CoronaVac was able to induce serum and salivary IgG anti-spike antibodies and IFN-γ producing T cells in most individuals who had recovered from COVID-19 and/or were vaccinated. Virus neutralizing activity was observed against the ancestral strain, with a reduced response against the variants. Vaccinated individuals who had previous COVID-19 presented higher responses than vaccinated individuals for all variables analysed. Our study provides evidence that the CoronaVac vaccine was able to induce the production of specific serum and saliva antibodies, serum virus neutralizing activity and cellular immune response, which were increased in previously COVID-19-infected individuals compared to uninfected individuals. |
| first_indexed | 2024-04-12T13:31:32Z |
| format | Article |
| id | doaj.art-e7b09225edbe4a0abd5d9657c0dc85f7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T13:31:32Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e7b09225edbe4a0abd5d9657c0dc85f72022-12-22T03:31:10ZengNature PortfolioScientific Reports2045-23222022-06-0112111210.1038/s41598-022-14283-xSalivary, serological, and cellular immune response to the CoronaVac vaccine in health care workers with or without previous COVID-19Marina Mazzilli Ortega0Laís Teodoro da Silva1Érika Donizetti Candido2Yingying Zheng3Bruna Tiaki Tiyo4Arthur Eduardo Fernandes Ferreira5Simone Corrêa-Silva6Guilherme Pereira Scagion7Fabyano Bruno Leal8Vanessa Nascimento Chalup9Camila Araújo Valério10Gabriela Justamante Händel Schmitz11Carina Ceneviva12Aline Pivetta Corá13Alexandre de Almeida14Edison Luiz Durigon15Danielle Bruna Leal Oliveira16Patricia Palmeira17Alberto José da Silva Duarte18Magda Carneiro-Sampaio19Telma Miyuki Oshiro20Laboratorio de Investigacao Medica em Dermatologia e Imunodeficiencias (LIM 56), Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloLaboratorio de Investigacao Medica em Dermatologia e Imunodeficiencias (LIM 56), Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloLaboratorio de Virologia Clinica e Molecular do Instituto de Ciencias Biomedicas da Universidade de São PauloDepartamento de Pediatria, Faculdade de Medicina, Universidade de Sao PauloLaboratorio de Investigacao Medica em Dermatologia e Imunodeficiencias (LIM 56), Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloLaboratorio de Pediatria Clinica (LIM 36), Departamento de Pediatria, Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloDepartamento de Pediatria, Faculdade de Medicina, Universidade de Sao PauloLaboratorio de Virologia Clinica e Molecular do Instituto de Ciencias Biomedicas da Universidade de São PauloLaboratorio de Virologia Clinica e Molecular do Instituto de Ciencias Biomedicas da Universidade de São PauloLaboratorio de Virologia Clinica e Molecular do Instituto de Ciencias Biomedicas da Universidade de São PauloLaboratorio de Virologia Clinica e Molecular do Instituto de Ciencias Biomedicas da Universidade de São PauloLaboratorio de Investigacao Medica em Dermatologia e Imunodeficiencias (LIM 56), Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloDivisao de Laboratorio Central, Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloDivisao de Laboratorio Central, Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloLaboratorio de Investigacao Medica em Dermatologia e Imunodeficiencias (LIM 56), Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloLaboratorio de Virologia Clinica e Molecular do Instituto de Ciencias Biomedicas da Universidade de São PauloLaboratorio de Virologia Clinica e Molecular do Instituto de Ciencias Biomedicas da Universidade de São PauloLaboratorio de Pediatria Clinica (LIM 36), Departamento de Pediatria, Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloLaboratorio de Investigacao Medica em Dermatologia e Imunodeficiencias (LIM 56), Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloDepartamento de Pediatria, Faculdade de Medicina, Universidade de Sao PauloLaboratorio de Investigacao Medica em Dermatologia e Imunodeficiencias (LIM 56), Faculdade de Medicina, Hospital das Clinicas HCFMUSP, Universidade de Sao PauloAbstract We investigated the anti-SARS-CoV-2 post-vaccine response through serum and salivary antibodies, serum antibody neutralizing activity and cellular immune response in samples from health care workers who were immunized with two doses of an inactivated virus-based vaccine (CoronaVac) who had or did not have COVID-19 previously. IgA and IgG antibodies directed at the spike protein were analysed in samples of saliva and/or serum by ELISA and/or chemiluminescence assays; the neutralizing activity of serum antibodies against reference strain B, Gamma and Delta SARS-CoV-2 variants were evaluated using a virus neutralization test and SARS-CoV-2 reactive interferon-gamma T-cell were analysed by flow cytometry. CoronaVac was able to induce serum and salivary IgG anti-spike antibodies and IFN-γ producing T cells in most individuals who had recovered from COVID-19 and/or were vaccinated. Virus neutralizing activity was observed against the ancestral strain, with a reduced response against the variants. Vaccinated individuals who had previous COVID-19 presented higher responses than vaccinated individuals for all variables analysed. Our study provides evidence that the CoronaVac vaccine was able to induce the production of specific serum and saliva antibodies, serum virus neutralizing activity and cellular immune response, which were increased in previously COVID-19-infected individuals compared to uninfected individuals.https://doi.org/10.1038/s41598-022-14283-x |
| spellingShingle | Marina Mazzilli Ortega Laís Teodoro da Silva Érika Donizetti Candido Yingying Zheng Bruna Tiaki Tiyo Arthur Eduardo Fernandes Ferreira Simone Corrêa-Silva Guilherme Pereira Scagion Fabyano Bruno Leal Vanessa Nascimento Chalup Camila Araújo Valério Gabriela Justamante Händel Schmitz Carina Ceneviva Aline Pivetta Corá Alexandre de Almeida Edison Luiz Durigon Danielle Bruna Leal Oliveira Patricia Palmeira Alberto José da Silva Duarte Magda Carneiro-Sampaio Telma Miyuki Oshiro Salivary, serological, and cellular immune response to the CoronaVac vaccine in health care workers with or without previous COVID-19 Scientific Reports |
| title | Salivary, serological, and cellular immune response to the CoronaVac vaccine in health care workers with or without previous COVID-19 |
| title_full | Salivary, serological, and cellular immune response to the CoronaVac vaccine in health care workers with or without previous COVID-19 |
| title_fullStr | Salivary, serological, and cellular immune response to the CoronaVac vaccine in health care workers with or without previous COVID-19 |
| title_full_unstemmed | Salivary, serological, and cellular immune response to the CoronaVac vaccine in health care workers with or without previous COVID-19 |
| title_short | Salivary, serological, and cellular immune response to the CoronaVac vaccine in health care workers with or without previous COVID-19 |
| title_sort | salivary serological and cellular immune response to the coronavac vaccine in health care workers with or without previous covid 19 |
| url | https://doi.org/10.1038/s41598-022-14283-x |
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