Association of Leptin Promoter 2548G/A variant with Serum Leptin, Lipid Profile and Type 2 Diabetes Mellitus
Background: A common 2548G/A promoter variant of the human leptin gene has been implicated in circulating leptin levels variations and pathogenesis of type 2 diabetes mellitus but available data are still conflicting. Objective: To explore potential associations between LEP 2548G/A of leptin w...
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| Format: | Article |
| Language: | English |
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College of medicine/ University of Diyala
2020-10-01
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| Series: | Diyala Journal of Medicine |
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| Online Access: | https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/590 |
| _version_ | 1797569085268033536 |
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| author | Gaylany H Abdullah |
| author_facet | Gaylany H Abdullah |
| author_sort | Gaylany H Abdullah |
| collection | DOAJ |
| description |
Background: A common 2548G/A promoter variant of the human leptin gene has been implicated in circulating leptin levels variations and pathogenesis of type 2 diabetes mellitus but available data are still conflicting.
Objective: To explore potential associations between LEP 2548G/A of leptin with T2DM and the effect of this variation on serum leptin levels.
Patients and Methods: Sixty two patients with T2DM and fifty non-diabetic controls were included in the study. Blood samples were collected from subjects for biochemical analysis and Genotyping was performed by polymerase chain reaction reaction–restriction fragment length polymorphism (PCR-RFLP).
Results: The genotype frequencies for LEP 2548G/G, LEP 2548G/A LEP 2548A/A were 46%, 32% and 22% in control group, and 19.4%, 33.9% and 46.7% in diabetic group, respectively. The A allele and GA/AA genotype of LEP 2548G/A was found to be more frequent than the G allele and GG genotype in T2DM patients compares to the controls. Subjects with the GA + AA genotype of LEP 2548G/A were at increased risk for T2DM (P=0.0001, OR = 1.78; 95% CI: 1.19–2.38). The serum leptin concentration of GA + AA genotype carriers was significantly higher from that of the GG genotype in the diabetic group.
Conclusion: A allele carrier who have higher serum concentrations of leptin may have an association with the risk of T2DM development in the Erbi population.
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| first_indexed | 2024-03-10T20:05:59Z |
| format | Article |
| id | doaj.art-e7b1a62589064a9481d37c94a16a7899 |
| institution | Directory Open Access Journal |
| issn | 2219-9764 2617-8982 |
| language | English |
| last_indexed | 2024-03-10T20:05:59Z |
| publishDate | 2020-10-01 |
| publisher | College of medicine/ University of Diyala |
| record_format | Article |
| series | Diyala Journal of Medicine |
| spelling | doaj.art-e7b1a62589064a9481d37c94a16a78992023-11-19T23:19:59ZengCollege of medicine/ University of DiyalaDiyala Journal of Medicine2219-97642617-89822020-10-01191Association of Leptin Promoter 2548G/A variant with Serum Leptin, Lipid Profile and Type 2 Diabetes Mellitus Gaylany H Abdullah Background: A common 2548G/A promoter variant of the human leptin gene has been implicated in circulating leptin levels variations and pathogenesis of type 2 diabetes mellitus but available data are still conflicting. Objective: To explore potential associations between LEP 2548G/A of leptin with T2DM and the effect of this variation on serum leptin levels. Patients and Methods: Sixty two patients with T2DM and fifty non-diabetic controls were included in the study. Blood samples were collected from subjects for biochemical analysis and Genotyping was performed by polymerase chain reaction reaction–restriction fragment length polymorphism (PCR-RFLP). Results: The genotype frequencies for LEP 2548G/G, LEP 2548G/A LEP 2548A/A were 46%, 32% and 22% in control group, and 19.4%, 33.9% and 46.7% in diabetic group, respectively. The A allele and GA/AA genotype of LEP 2548G/A was found to be more frequent than the G allele and GG genotype in T2DM patients compares to the controls. Subjects with the GA + AA genotype of LEP 2548G/A were at increased risk for T2DM (P=0.0001, OR = 1.78; 95% CI: 1.19–2.38). The serum leptin concentration of GA + AA genotype carriers was significantly higher from that of the GG genotype in the diabetic group. Conclusion: A allele carrier who have higher serum concentrations of leptin may have an association with the risk of T2DM development in the Erbi population. https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/590Leptin, single nucleotide polymorphism, polymerase chain reaction– restriction fragment length polymorphism,T2DM |
| spellingShingle | Gaylany H Abdullah Association of Leptin Promoter 2548G/A variant with Serum Leptin, Lipid Profile and Type 2 Diabetes Mellitus Diyala Journal of Medicine Leptin, single nucleotide polymorphism, polymerase chain reaction– restriction fragment length polymorphism,T2DM |
| title | Association of Leptin Promoter 2548G/A variant with Serum Leptin, Lipid Profile and Type 2 Diabetes Mellitus |
| title_full | Association of Leptin Promoter 2548G/A variant with Serum Leptin, Lipid Profile and Type 2 Diabetes Mellitus |
| title_fullStr | Association of Leptin Promoter 2548G/A variant with Serum Leptin, Lipid Profile and Type 2 Diabetes Mellitus |
| title_full_unstemmed | Association of Leptin Promoter 2548G/A variant with Serum Leptin, Lipid Profile and Type 2 Diabetes Mellitus |
| title_short | Association of Leptin Promoter 2548G/A variant with Serum Leptin, Lipid Profile and Type 2 Diabetes Mellitus |
| title_sort | association of leptin promoter 2548g a variant with serum leptin lipid profile and type 2 diabetes mellitus |
| topic | Leptin, single nucleotide polymorphism, polymerase chain reaction– restriction fragment length polymorphism,T2DM |
| url | https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/590 |
| work_keys_str_mv | AT gaylanyhabdullah associationofleptinpromoter2548gavariantwithserumleptinlipidprofileandtype2diabetesmellitus |