The spike protein of SARS-CoV-2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticles
Abstract SARS-CoV-2 is a matter of concern. Here, biosensors were prepared using iron magnetic nanoparticles containing antibodies against the receptor binding domain (RBD) of the spike protein. Antibodies were adsorbed to nanoparticles in three configurations, including direct adsorption without fu...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2023-07-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652023000200501&lng=en&tlng=en |
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| author | JULIUS JOSÉ T.J. PAUL SAMARA L.C. MAROUN WYLLERSON EVARISTO GOMES LETÍCIA O. DO CARMO RENATA KELLY MENDES AUGUSTO ETCHEGARAY |
| author_facet | JULIUS JOSÉ T.J. PAUL SAMARA L.C. MAROUN WYLLERSON EVARISTO GOMES LETÍCIA O. DO CARMO RENATA KELLY MENDES AUGUSTO ETCHEGARAY |
| author_sort | JULIUS JOSÉ T.J. PAUL |
| collection | DOAJ |
| description | Abstract SARS-CoV-2 is a matter of concern. Here, biosensors were prepared using iron magnetic nanoparticles containing antibodies against the receptor binding domain (RBD) of the spike protein. Antibodies were adsorbed to nanoparticles in three configurations, including direct adsorption without functionalization (DANPs). Nanoparticles were added to a glassy carbon electrode and connected to an electrochemical cell. Electrochemical impedance spectroscopy and ELISA experiments indicated that antibodies were desorbed from the DANPs upon the addition of the RBD. DANPs-based biosensors produced linear curves with decreasing charge transfer resistance due to the removal of antibodies. Thus, a detection method can be based on antibody desorption. |
| first_indexed | 2024-03-13T00:24:39Z |
| format | Article |
| id | doaj.art-e7b53c7f4f114ee7b43c095a081e58b2 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-03-13T00:24:39Z |
| publishDate | 2023-07-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-e7b53c7f4f114ee7b43c095a081e58b22023-07-11T07:48:37ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902023-07-0195suppl 110.1590/0001-3765202320220624The spike protein of SARS-CoV-2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticlesJULIUS JOSÉ T.J. PAULhttps://orcid.org/0000-0002-9210-5082SAMARA L.C. MAROUNhttps://orcid.org/0000-0003-4046-9025WYLLERSON EVARISTO GOMEShttps://orcid.org/0000-0002-1725-6921LETÍCIA O. DO CARMOhttps://orcid.org/0000-0002-6414-0366RENATA KELLY MENDEShttps://orcid.org/0000-0001-8329-5518AUGUSTO ETCHEGARAYhttps://orcid.org/0000-0002-0483-0373Abstract SARS-CoV-2 is a matter of concern. Here, biosensors were prepared using iron magnetic nanoparticles containing antibodies against the receptor binding domain (RBD) of the spike protein. Antibodies were adsorbed to nanoparticles in three configurations, including direct adsorption without functionalization (DANPs). Nanoparticles were added to a glassy carbon electrode and connected to an electrochemical cell. Electrochemical impedance spectroscopy and ELISA experiments indicated that antibodies were desorbed from the DANPs upon the addition of the RBD. DANPs-based biosensors produced linear curves with decreasing charge transfer resistance due to the removal of antibodies. Thus, a detection method can be based on antibody desorption.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652023000200501&lng=en&tlng=enBiosensorelectrochemical impedance spectroscopyspike proteinreceptor binding domainCOVID-19SARS-CoV-2 |
| spellingShingle | JULIUS JOSÉ T.J. PAUL SAMARA L.C. MAROUN WYLLERSON EVARISTO GOMES LETÍCIA O. DO CARMO RENATA KELLY MENDES AUGUSTO ETCHEGARAY The spike protein of SARS-CoV-2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticles Anais da Academia Brasileira de Ciências Biosensor electrochemical impedance spectroscopy spike protein receptor binding domain COVID-19 SARS-CoV-2 |
| title | The spike protein of SARS-CoV-2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticles |
| title_full | The spike protein of SARS-CoV-2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticles |
| title_fullStr | The spike protein of SARS-CoV-2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticles |
| title_full_unstemmed | The spike protein of SARS-CoV-2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticles |
| title_short | The spike protein of SARS-CoV-2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticles |
| title_sort | spike protein of sars cov 2 can be detected by electrochemical impedance spectroscopy using antibody desorption from iron magnetic nanoparticles |
| topic | Biosensor electrochemical impedance spectroscopy spike protein receptor binding domain COVID-19 SARS-CoV-2 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652023000200501&lng=en&tlng=en |
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