Polymorphism of coagulase gene in Staphylococcus aureus isolated from buffaloe milk

The aim of this study was to analyze the coA gene on Staphylococcus aureus isolates collected from milks of native buffaloes in north-west of Iran. For this purpose, seventy five S. aureus isolates were examined by PCR-RFLP. Amplification of coA gene revealed various amplicons with a sizes of approx...

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Bibliographic Details
Main Authors: J Shayegh, A.R Monadi
Format: Article
Language:fas
Published: Islamic Azad University, Tabriz Branch 2013-05-01
Series:Bihdāsht-i Mavādd-i Ghaz̠āyī
Subjects:
Online Access:http://jfh.iaut.ac.ir/article_517601_a486e601f5e6f92675c271eaade3d047.pdf
Description
Summary:The aim of this study was to analyze the coA gene on Staphylococcus aureus isolates collected from milks of native buffaloes in north-west of Iran. For this purpose, seventy five S. aureus isolates were examined by PCR-RFLP. Amplification of coA gene revealed various amplicons with a sizes of approximately 600, 700, 760 and 850 bp in 12, 25, 29 and 9 of the isolates, respectively. AluI digestion of the amplicons demonstrated that the isolates had the same PCR band size in the same PCR-RFLP patterns. The result of coA gen analysis was similar to the isolates from cattle milk.
ISSN:2228-7647
2476-6968