The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions
Aronia is a berry fruit that has a significant amount of antioxidants. Both sexual and vegetative methods of propagation are available for aronia. Genetic diversity is present in the process of seed propagation. Therefore, clonal propagation techniques such as cuttings, basal shoots, and tissue cul...
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| Format: | Article |
| Language: | English |
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AcademicPres
2024-03-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13542 |
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| author | Heydem EKINCI Necla SASKIN Şehnaz KORKMAZ Bekir E. AK Yeter AYDINLIK |
| author_facet | Heydem EKINCI Necla SASKIN Şehnaz KORKMAZ Bekir E. AK Yeter AYDINLIK |
| author_sort | Heydem EKINCI |
| collection | DOAJ |
| description |
Aronia is a berry fruit that has a significant amount of antioxidants. Both sexual and vegetative methods of propagation are available for aronia. Genetic diversity is present in the process of seed propagation. Therefore, clonal propagation techniques such as cuttings, basal shoots, and tissue culture are preferred. Plant tissue culture is a process that offers a high multiplication rate and the ability to produce clean plant material. This study aimed to stimulate the growth of plantlets by introducing several growth-promoting agents into the culture media. Specifically, the effects of a nitric oxide donor SNP (sodium nitroprusside) on the vegetative development of the plants were examined under in vitro conditions. Four distinct concentrations of SNP (0, 100, 200, and 300 µM) were administered to promote vegetative development. The survival rate (%), rooting rate (%), root number (per plantlet), root length (cm), leaf number (per plantlet), shoot length (cm), plant fresh weight (g), and plant dry weight (g) of the plantlets were estimated on the 60th day of growth in the nutrient medium under in vitro conditions. The survival rate was 100% for the applications of SNP at concentrations of 100 µM and 200 µM. The 0 µM treatment had the highest root number, the 100 µM treatment had the longest root, and the 200 µM treatment had the highest values for shoot length. The 0 µM treatment provided the highest values for plant fresh weight, while the 0 and 100 µM applications resulted in the highest plant dry weight values. The study suggested that an increased concentration of SNP causes a toxic effect.
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| first_indexed | 2024-04-24T15:43:18Z |
| format | Article |
| id | doaj.art-e7b98c1df2a74761aede06e8674ed1c2 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-04-24T15:43:18Z |
| publishDate | 2024-03-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-e7b98c1df2a74761aede06e8674ed1c22024-04-01T18:46:34ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092024-03-0152110.15835/nbha52113542The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditionsHeydem EKINCI0Necla SASKIN1Şehnaz KORKMAZ2Bekir E. AK3Yeter AYDINLIK4Harran University, Faculty of Agriculture, Department of Horticulture, 63050, Haliliye, SanliurfaHarran University, Faculty of Agriculture, Department of Horticulture, 63050, Haliliye, SanliurfaMinistry of Agriculture and Forestry, GAP Agricultural Research Institute, Department of Horticulture, 63040, Haliliye, SanliurfaHarran University, Faculty of Agriculture, Department of Horticulture, 63050, Haliliye, SanliurfaHarran University, Faculty of Agriculture, Department of Horticulture, 63050, Haliliye, Sanliurfa Aronia is a berry fruit that has a significant amount of antioxidants. Both sexual and vegetative methods of propagation are available for aronia. Genetic diversity is present in the process of seed propagation. Therefore, clonal propagation techniques such as cuttings, basal shoots, and tissue culture are preferred. Plant tissue culture is a process that offers a high multiplication rate and the ability to produce clean plant material. This study aimed to stimulate the growth of plantlets by introducing several growth-promoting agents into the culture media. Specifically, the effects of a nitric oxide donor SNP (sodium nitroprusside) on the vegetative development of the plants were examined under in vitro conditions. Four distinct concentrations of SNP (0, 100, 200, and 300 µM) were administered to promote vegetative development. The survival rate (%), rooting rate (%), root number (per plantlet), root length (cm), leaf number (per plantlet), shoot length (cm), plant fresh weight (g), and plant dry weight (g) of the plantlets were estimated on the 60th day of growth in the nutrient medium under in vitro conditions. The survival rate was 100% for the applications of SNP at concentrations of 100 µM and 200 µM. The 0 µM treatment had the highest root number, the 100 µM treatment had the longest root, and the 200 µM treatment had the highest values for shoot length. The 0 µM treatment provided the highest values for plant fresh weight, while the 0 and 100 µM applications resulted in the highest plant dry weight values. The study suggested that an increased concentration of SNP causes a toxic effect. https://www.notulaebotanicae.ro/index.php/nbha/article/view/13542Aronia melanocarpa [Michx.] Elliotin vitroregenerationsodium nitroprussidevegetative development |
| spellingShingle | Heydem EKINCI Necla SASKIN Şehnaz KORKMAZ Bekir E. AK Yeter AYDINLIK The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions Notulae Botanicae Horti Agrobotanici Cluj-Napoca Aronia melanocarpa [Michx.] Elliot in vitro regeneration sodium nitroprusside vegetative development |
| title | The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions |
| title_full | The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions |
| title_fullStr | The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions |
| title_full_unstemmed | The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions |
| title_short | The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions |
| title_sort | effect of sodium nitroprusside on the vegetative development of aronia melanocarpa michx elliot under in vitro conditions |
| topic | Aronia melanocarpa [Michx.] Elliot in vitro regeneration sodium nitroprusside vegetative development |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13542 |
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