<i>Saccharomyces cerevisiae</i> and <i>Candida albicans</i> Yeast Cells Labeled with Fe(III) Complexes as MRI Probes
The development of MRI probes is of interest for labeling antibiotic-resistant fungal infections based on yeast. Our work showed that yeast cells can be labeled with high-spin Fe(III) complexes to produce enhanced T<sub>2</sub> water proton relaxation. These Fe(III)-based macrocyclic com...
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MDPI AG
2020-09-01
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| Series: | Magnetochemistry |
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| Online Access: | https://www.mdpi.com/2312-7481/6/3/41 |
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| author | Akanksha Patel Didar Asik Eric M. Snyder Joseph A. Spernyak Paul J. Cullen Janet R. Morrow |
| author_facet | Akanksha Patel Didar Asik Eric M. Snyder Joseph A. Spernyak Paul J. Cullen Janet R. Morrow |
| author_sort | Akanksha Patel |
| collection | DOAJ |
| description | The development of MRI probes is of interest for labeling antibiotic-resistant fungal infections based on yeast. Our work showed that yeast cells can be labeled with high-spin Fe(III) complexes to produce enhanced T<sub>2</sub> water proton relaxation. These Fe(III)-based macrocyclic complexes contained a 1,4,7-triazacyclononane framework, two pendant alcohol groups, and either a non-coordinating ancillary group and a bound water molecule or a third coordinating pendant. The Fe(III) complexes that had an open coordination site associated strongly with <i>Saccharomyces cerevisiae</i> upon incubation, as shown by screening using Z-spectra analysis. The incubation of one Fe(III) complex with either <i>Saccharomyces cerevisiae</i> or <i>Candida albicans</i> yeast led to an interaction with the β-glucan-based cell wall, as shown by the ready retrieval of the complex by the bidentate chelator called maltol. Other conditions, such as a heat shock treatment of the complexes, produced Fe(III) complex uptake that could not be reversed by the addition of maltol. Appending a fluorescence dye to Fe(TOB) led to uptake through secretory pathways, as shown by confocal fluorescence microscopy and by the incomplete retrieval of the Fe(III) complex by the maltol treatment. Yeast cells that were labeled with these Fe(III) complexes displayed enhanced water proton T<sub>2</sub> relaxation, both for <i>S. cerevisiae</i> and for yeast and hyphal forms of <i>C. albicans</i>. |
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| issn | 2312-7481 |
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| last_indexed | 2024-03-10T16:34:22Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Magnetochemistry |
| spelling | doaj.art-e7bb30bf1b5341f683e84fa92e0e24ae2023-11-20T12:36:27ZengMDPI AGMagnetochemistry2312-74812020-09-01634110.3390/magnetochemistry6030041<i>Saccharomyces cerevisiae</i> and <i>Candida albicans</i> Yeast Cells Labeled with Fe(III) Complexes as MRI ProbesAkanksha Patel0Didar Asik1Eric M. Snyder2Joseph A. Spernyak3Paul J. Cullen4Janet R. Morrow5Department of Chemistry, University at Buffalo, The State University of New York, Amherst, NY 14260, USADepartment of Chemistry, University at Buffalo, The State University of New York, Amherst, NY 14260, USADepartment of Chemistry, University at Buffalo, The State University of New York, Amherst, NY 14260, USADepartment of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo NY 14263, USADepartment of Biology, University at Buffalo, The State University of New York, Amherst, NY 14260, USADepartment of Chemistry, University at Buffalo, The State University of New York, Amherst, NY 14260, USAThe development of MRI probes is of interest for labeling antibiotic-resistant fungal infections based on yeast. Our work showed that yeast cells can be labeled with high-spin Fe(III) complexes to produce enhanced T<sub>2</sub> water proton relaxation. These Fe(III)-based macrocyclic complexes contained a 1,4,7-triazacyclononane framework, two pendant alcohol groups, and either a non-coordinating ancillary group and a bound water molecule or a third coordinating pendant. The Fe(III) complexes that had an open coordination site associated strongly with <i>Saccharomyces cerevisiae</i> upon incubation, as shown by screening using Z-spectra analysis. The incubation of one Fe(III) complex with either <i>Saccharomyces cerevisiae</i> or <i>Candida albicans</i> yeast led to an interaction with the β-glucan-based cell wall, as shown by the ready retrieval of the complex by the bidentate chelator called maltol. Other conditions, such as a heat shock treatment of the complexes, produced Fe(III) complex uptake that could not be reversed by the addition of maltol. Appending a fluorescence dye to Fe(TOB) led to uptake through secretory pathways, as shown by confocal fluorescence microscopy and by the incomplete retrieval of the Fe(III) complex by the maltol treatment. Yeast cells that were labeled with these Fe(III) complexes displayed enhanced water proton T<sub>2</sub> relaxation, both for <i>S. cerevisiae</i> and for yeast and hyphal forms of <i>C. albicans</i>.https://www.mdpi.com/2312-7481/6/3/41Fe(III) complexescell labelingT<sub>2</sub> contrastinvasive candidiasisMRI |
| spellingShingle | Akanksha Patel Didar Asik Eric M. Snyder Joseph A. Spernyak Paul J. Cullen Janet R. Morrow <i>Saccharomyces cerevisiae</i> and <i>Candida albicans</i> Yeast Cells Labeled with Fe(III) Complexes as MRI Probes Magnetochemistry Fe(III) complexes cell labeling T<sub>2</sub> contrast invasive candidiasis MRI |
| title | <i>Saccharomyces cerevisiae</i> and <i>Candida albicans</i> Yeast Cells Labeled with Fe(III) Complexes as MRI Probes |
| title_full | <i>Saccharomyces cerevisiae</i> and <i>Candida albicans</i> Yeast Cells Labeled with Fe(III) Complexes as MRI Probes |
| title_fullStr | <i>Saccharomyces cerevisiae</i> and <i>Candida albicans</i> Yeast Cells Labeled with Fe(III) Complexes as MRI Probes |
| title_full_unstemmed | <i>Saccharomyces cerevisiae</i> and <i>Candida albicans</i> Yeast Cells Labeled with Fe(III) Complexes as MRI Probes |
| title_short | <i>Saccharomyces cerevisiae</i> and <i>Candida albicans</i> Yeast Cells Labeled with Fe(III) Complexes as MRI Probes |
| title_sort | i saccharomyces cerevisiae i and i candida albicans i yeast cells labeled with fe iii complexes as mri probes |
| topic | Fe(III) complexes cell labeling T<sub>2</sub> contrast invasive candidiasis MRI |
| url | https://www.mdpi.com/2312-7481/6/3/41 |
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