Molecular coordination of Staphylococcus aureus cell division
The bacterial cell wall is essential for viability, but despite its ability to withstand internal turgor must remain dynamic to permit growth and division. Peptidoglycan is the major cell wall structural polymer, whose synthesis requires multiple interacting components. The human pathogen Staphyloco...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/32057 |
| _version_ | 1811200070731694080 |
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| author | Victoria A Lund Katarzyna Wacnik Robert D Turner Bryony E Cotterell Christa G Walther Samuel J Fenn Fabian Grein Adam JM Wollman Mark C Leake Nicolas Olivier Ashley Cadby Stéphane Mesnage Simon Jones Simon J Foster |
| author_facet | Victoria A Lund Katarzyna Wacnik Robert D Turner Bryony E Cotterell Christa G Walther Samuel J Fenn Fabian Grein Adam JM Wollman Mark C Leake Nicolas Olivier Ashley Cadby Stéphane Mesnage Simon Jones Simon J Foster |
| author_sort | Victoria A Lund |
| collection | DOAJ |
| description | The bacterial cell wall is essential for viability, but despite its ability to withstand internal turgor must remain dynamic to permit growth and division. Peptidoglycan is the major cell wall structural polymer, whose synthesis requires multiple interacting components. The human pathogen Staphylococcus aureus is a prolate spheroid that divides in three orthogonal planes. Here, we have integrated cellular morphology during division with molecular level resolution imaging of peptidoglycan synthesis and the components responsible. Synthesis occurs across the developing septal surface in a diffuse pattern, a necessity of the observed septal geometry, that is matched by variegated division component distribution. Synthesis continues after septal annulus completion, where the core division component FtsZ remains. The novel molecular level information requires re-evaluation of the growth and division processes leading to a new conceptual model, whereby the cell cycle is expedited by a set of functionally connected but not regularly distributed components. |
| first_indexed | 2024-04-12T01:58:35Z |
| format | Article |
| id | doaj.art-e7bf4c1659a047a0972561a7ebc7c0c9 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:58:35Z |
| publishDate | 2018-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e7bf4c1659a047a0972561a7ebc7c0c92022-12-22T03:52:45ZengeLife Sciences Publications LtdeLife2050-084X2018-02-01710.7554/eLife.32057Molecular coordination of Staphylococcus aureus cell divisionVictoria A Lund0https://orcid.org/0000-0002-1637-2023Katarzyna Wacnik1https://orcid.org/0000-0002-9921-6746Robert D Turner2Bryony E Cotterell3Christa G Walther4https://orcid.org/0000-0002-8962-3102Samuel J Fenn5Fabian Grein6Adam JM Wollman7https://orcid.org/0000-0002-5501-8131Mark C Leake8https://orcid.org/0000-0002-1715-1249Nicolas Olivier9Ashley Cadby10Stéphane Mesnage11Simon Jones12https://orcid.org/0000-0001-8043-7998Simon J Foster13https://orcid.org/0000-0001-7432-7805Krebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United KingdomKrebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United KingdomKrebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom; Department of Physics and Astronomy, University of Sheffield, Sheffield, United KingdomKrebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom; Department of Chemistry, University of Sheffield, Sheffield, United KingdomKrebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United KingdomKrebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United KingdomInstitute for Pharmaceutical Microbiology, German Center for Infection Research (DZIF), University of Bonn, Bonn, GermanyBiological Physical Sciences Institute, University of York, York, United KingdomBiological Physical Sciences Institute, University of York, York, United KingdomKrebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Physics and Astronomy, University of Sheffield, Sheffield, United KingdomKrebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Physics and Astronomy, University of Sheffield, Sheffield, United KingdomKrebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United KingdomDepartment of Chemistry, University of Sheffield, Sheffield, United KingdomKrebs Institute, University of Sheffield, Sheffield, United Kingdom; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United KingdomThe bacterial cell wall is essential for viability, but despite its ability to withstand internal turgor must remain dynamic to permit growth and division. Peptidoglycan is the major cell wall structural polymer, whose synthesis requires multiple interacting components. The human pathogen Staphylococcus aureus is a prolate spheroid that divides in three orthogonal planes. Here, we have integrated cellular morphology during division with molecular level resolution imaging of peptidoglycan synthesis and the components responsible. Synthesis occurs across the developing septal surface in a diffuse pattern, a necessity of the observed septal geometry, that is matched by variegated division component distribution. Synthesis continues after septal annulus completion, where the core division component FtsZ remains. The novel molecular level information requires re-evaluation of the growth and division processes leading to a new conceptual model, whereby the cell cycle is expedited by a set of functionally connected but not regularly distributed components.https://elifesciences.org/articles/32057Staphylococcus aureusPeptidoglycanDivisionCell wallDivisome |
| spellingShingle | Victoria A Lund Katarzyna Wacnik Robert D Turner Bryony E Cotterell Christa G Walther Samuel J Fenn Fabian Grein Adam JM Wollman Mark C Leake Nicolas Olivier Ashley Cadby Stéphane Mesnage Simon Jones Simon J Foster Molecular coordination of Staphylococcus aureus cell division eLife Staphylococcus aureus Peptidoglycan Division Cell wall Divisome |
| title | Molecular coordination of Staphylococcus aureus cell division |
| title_full | Molecular coordination of Staphylococcus aureus cell division |
| title_fullStr | Molecular coordination of Staphylococcus aureus cell division |
| title_full_unstemmed | Molecular coordination of Staphylococcus aureus cell division |
| title_short | Molecular coordination of Staphylococcus aureus cell division |
| title_sort | molecular coordination of staphylococcus aureus cell division |
| topic | Staphylococcus aureus Peptidoglycan Division Cell wall Divisome |
| url | https://elifesciences.org/articles/32057 |
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