Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>
Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from <i>Fucus vesiculosus</i> using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol...
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MDPI AG
2020-11-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/11/559 |
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| author | Sónia J. Amarante Marcelo D. Catarino Catarina Marçal Artur M. S. Silva Rita Ferreira Susana M. Cardoso |
| author_facet | Sónia J. Amarante Marcelo D. Catarino Catarina Marçal Artur M. S. Silva Rita Ferreira Susana M. Cardoso |
| author_sort | Sónia J. Amarante |
| collection | DOAJ |
| description | Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from <i>Fucus vesiculosus</i> using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (<i>v/v</i>), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DW<sub>extract</sub>), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS<sup>•+</sup> (IC<sub>50</sub> of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC<sub>50</sub> of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose. |
| first_indexed | 2024-03-10T14:51:19Z |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T14:51:19Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e7c0cacac3694850bae3fce70a88d3a92023-11-20T21:02:02ZengMDPI AGMarine Drugs1660-33972020-11-01181155910.3390/md18110559Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>Sónia J. Amarante0Marcelo D. Catarino1Catarina Marçal2Artur M. S. Silva3Rita Ferreira4Susana M. Cardoso5LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalMicrowave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from <i>Fucus vesiculosus</i> using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (<i>v/v</i>), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DW<sub>extract</sub>), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS<sup>•+</sup> (IC<sub>50</sub> of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC<sub>50</sub> of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose.https://www.mdpi.com/1660-3397/18/11/559brown seaweedsphlorotanninsmicrowave-assisted extractionresponse surface methodologyantioxidantantiradical activity |
| spellingShingle | Sónia J. Amarante Marcelo D. Catarino Catarina Marçal Artur M. S. Silva Rita Ferreira Susana M. Cardoso Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> Marine Drugs brown seaweeds phlorotannins microwave-assisted extraction response surface methodology antioxidant antiradical activity |
| title | Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
| title_full | Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
| title_fullStr | Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
| title_full_unstemmed | Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
| title_short | Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
| title_sort | microwave assisted extraction of phlorotannins from i fucus vesiculosus i |
| topic | brown seaweeds phlorotannins microwave-assisted extraction response surface methodology antioxidant antiradical activity |
| url | https://www.mdpi.com/1660-3397/18/11/559 |
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