Effect of Intramammary Dry Cow Antimicrobial Treatment on Fresh Cow’s Milk Microbiota in California Commercial Dairies
This study used 16S rRNA sequencing to evaluate the effects of dry cow antimicrobial therapy on the udder milk microbiota by comparing the microbial populations in milk at dry-off (DRY) (~60 days before calving) and post-partum (FRESH) (4–11 days after calving) from cows receiving an intramammary an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/7/963 |
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| author | Carl Basbas Sharif Aly Emmanuel Okello Betsy M. Karle Terry Lehenbauer Deniece Williams Erika Ganda Martin Wiedmann Richard V. Pereira |
| author_facet | Carl Basbas Sharif Aly Emmanuel Okello Betsy M. Karle Terry Lehenbauer Deniece Williams Erika Ganda Martin Wiedmann Richard V. Pereira |
| author_sort | Carl Basbas |
| collection | DOAJ |
| description | This study used 16S rRNA sequencing to evaluate the effects of dry cow antimicrobial therapy on the udder milk microbiota by comparing the microbial populations in milk at dry-off (DRY) (~60 days before calving) and post-partum (FRESH) (4–11 days after calving) from cows receiving an intramammary antibiotic infusion prior to dry-off (IMT) and cows that did not receive treatment (CTL). Milk was collected from 23 cows from the IMT group and 27 cows from the CTL group. IMT and DRY samples had a greater correlation with the genera <i>Brevibacterium</i> and <i>Amaricoccus</i>, and the family Micrococcaceae, when compared to IMT and FRESH samples. CTL group samples collected at DRY had a greater correlation with the genera <i>Akkermansia</i> and <i>Syntrophus,</i> when compared to FRESH samples; no bacterial taxa were observed to have a significant correlation with FRESH samples in the CTL group. DRY samples collected from the CTL group had a greater correlation with the genus <i>Mogibacterium</i> when compared to IMT and CTL samples. For DRY samples collected from the IMT group, a greater correlation with the genus <i>Alkalibacterium</i> when compared to DRY and CTL samples, was observed. The lack of a correlation for FRESH samples between the CTL and IMT treatment groups indicated that intramammary antimicrobial dry cow therapy had no significant effect on the udder milk microbiota post-partum. |
| first_indexed | 2024-03-09T10:23:39Z |
| format | Article |
| id | doaj.art-e7c3379b1fc64df8b32adbceff55a25d |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T10:23:39Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-e7c3379b1fc64df8b32adbceff55a25d2023-12-01T21:49:12ZengMDPI AGAntibiotics2079-63822022-07-0111796310.3390/antibiotics11070963Effect of Intramammary Dry Cow Antimicrobial Treatment on Fresh Cow’s Milk Microbiota in California Commercial DairiesCarl Basbas0Sharif Aly1Emmanuel Okello2Betsy M. Karle3Terry Lehenbauer4Deniece Williams5Erika Ganda6Martin Wiedmann7Richard V. Pereira8Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USADepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USADepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USACooperative Extension, Division of Agriculture and Natural Resources, University of California, Orland, CA 95963, USADepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USAVeterinary Medicine Teaching and Research Center, University of California, Davis, Tulare, CA 93274, USADepartment of Food Science, Cornell University, Ithaca, NY 14850, USADepartment of Food Science, Cornell University, Ithaca, NY 14850, USADepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USAThis study used 16S rRNA sequencing to evaluate the effects of dry cow antimicrobial therapy on the udder milk microbiota by comparing the microbial populations in milk at dry-off (DRY) (~60 days before calving) and post-partum (FRESH) (4–11 days after calving) from cows receiving an intramammary antibiotic infusion prior to dry-off (IMT) and cows that did not receive treatment (CTL). Milk was collected from 23 cows from the IMT group and 27 cows from the CTL group. IMT and DRY samples had a greater correlation with the genera <i>Brevibacterium</i> and <i>Amaricoccus</i>, and the family Micrococcaceae, when compared to IMT and FRESH samples. CTL group samples collected at DRY had a greater correlation with the genera <i>Akkermansia</i> and <i>Syntrophus,</i> when compared to FRESH samples; no bacterial taxa were observed to have a significant correlation with FRESH samples in the CTL group. DRY samples collected from the CTL group had a greater correlation with the genus <i>Mogibacterium</i> when compared to IMT and CTL samples. For DRY samples collected from the IMT group, a greater correlation with the genus <i>Alkalibacterium</i> when compared to DRY and CTL samples, was observed. The lack of a correlation for FRESH samples between the CTL and IMT treatment groups indicated that intramammary antimicrobial dry cow therapy had no significant effect on the udder milk microbiota post-partum.https://www.mdpi.com/2079-6382/11/7/963milkmastitisantibioticsdry-off |
| spellingShingle | Carl Basbas Sharif Aly Emmanuel Okello Betsy M. Karle Terry Lehenbauer Deniece Williams Erika Ganda Martin Wiedmann Richard V. Pereira Effect of Intramammary Dry Cow Antimicrobial Treatment on Fresh Cow’s Milk Microbiota in California Commercial Dairies Antibiotics milk mastitis antibiotics dry-off |
| title | Effect of Intramammary Dry Cow Antimicrobial Treatment on Fresh Cow’s Milk Microbiota in California Commercial Dairies |
| title_full | Effect of Intramammary Dry Cow Antimicrobial Treatment on Fresh Cow’s Milk Microbiota in California Commercial Dairies |
| title_fullStr | Effect of Intramammary Dry Cow Antimicrobial Treatment on Fresh Cow’s Milk Microbiota in California Commercial Dairies |
| title_full_unstemmed | Effect of Intramammary Dry Cow Antimicrobial Treatment on Fresh Cow’s Milk Microbiota in California Commercial Dairies |
| title_short | Effect of Intramammary Dry Cow Antimicrobial Treatment on Fresh Cow’s Milk Microbiota in California Commercial Dairies |
| title_sort | effect of intramammary dry cow antimicrobial treatment on fresh cow s milk microbiota in california commercial dairies |
| topic | milk mastitis antibiotics dry-off |
| url | https://www.mdpi.com/2079-6382/11/7/963 |
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