Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion
The shortage of dog blood donors in veterinary emergencies can lead to blood transfusions between animals whose blood type has not been identified. The antibody profile serves as a warning sign for animals that require a second blood transfusion, which is only advisable from compatible donor dogs. T...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2018-04-01
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| Series: | Brazilian Journal of Veterinary Research and Animal Science |
| Subjects: | |
| Online Access: | http://www.revistas.usp.br/bjvras/article/view/122274 |
| _version_ | 1811331061688303616 |
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| author | Suzana Claudia Spinola dos Santos Ludmila Rodrigues Moroz Mariane Melo dos Santos Allan Souza dos Santos Soraya Castro Trindade Roberto Meyer Maria de Fátima Dias Costa |
| author_facet | Suzana Claudia Spinola dos Santos Ludmila Rodrigues Moroz Mariane Melo dos Santos Allan Souza dos Santos Soraya Castro Trindade Roberto Meyer Maria de Fátima Dias Costa |
| author_sort | Suzana Claudia Spinola dos Santos |
| collection | DOAJ |
| description | The shortage of dog blood donors in veterinary emergencies can lead to blood transfusions between animals whose blood type has not been identified. The antibody profile serves as a warning sign for animals that require a second blood transfusion, which is only advisable from compatible donor dogs. This article focuses on determination of anti-DEA 1 antibodies using the flow cytometry technique in dogs that have undergone a transfusion using DEA 1-positive blood, compared to results obtained from crossmatching. Blood from 18 DEA 1-positive donors ranked according to the chromatographic technique was used to transfuse thirty-three animals with unknown blood types and which demonstrated negative crossmatching to donors. On post-transfusion days 7, 14, 21 and 28, 45% and 27% of the animals tested positive for the anti-DEA 1 antibody, through crossmatching and flow cytometry, respectively. Detecting antibodies using the flow cytometric technique has high specificity and sensitivity, while crossmatching methods are highly sensitive but manifest low specificity. Following blood transfusion, animals that did not present as positive through crossmatching or flow cytometry were considered different from all other DEA 1-positive blood groups. |
| first_indexed | 2024-04-13T16:13:13Z |
| format | Article |
| id | doaj.art-e7c3c407e56541e397fc8d5ed6bd6b13 |
| institution | Directory Open Access Journal |
| issn | 1413-9596 1678-4456 |
| language | English |
| last_indexed | 2024-04-13T16:13:13Z |
| publishDate | 2018-04-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Veterinary Research and Animal Science |
| spelling | doaj.art-e7c3c407e56541e397fc8d5ed6bd6b132022-12-22T02:40:09ZengUniversidade de São PauloBrazilian Journal of Veterinary Research and Animal Science1413-95961678-44562018-04-0155110.11606/issn.1678-4456.bjvras.2018.122274Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusionSuzana Claudia Spinola dos Santos0Ludmila Rodrigues Moroz1Mariane Melo dos Santos2Allan Souza dos Santos3Soraya Castro Trindade4Roberto Meyer5Maria de Fátima Dias Costa6Universidade Federal da Bahia, Instituto de Ciências da Saúde, Programa de Pós-graduação em Processos Interativos dos Órgãos e SistemasUniversidade Federal da Bahia, Hospital de Medicina Veterinária, Laboratório de Patologia ClínicaUniversidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia MolecularUniversidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia MolecularUniversidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia MolecularUniversidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia MolecularUniversidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Neuroquímica e Biologia CelularThe shortage of dog blood donors in veterinary emergencies can lead to blood transfusions between animals whose blood type has not been identified. The antibody profile serves as a warning sign for animals that require a second blood transfusion, which is only advisable from compatible donor dogs. This article focuses on determination of anti-DEA 1 antibodies using the flow cytometry technique in dogs that have undergone a transfusion using DEA 1-positive blood, compared to results obtained from crossmatching. Blood from 18 DEA 1-positive donors ranked according to the chromatographic technique was used to transfuse thirty-three animals with unknown blood types and which demonstrated negative crossmatching to donors. On post-transfusion days 7, 14, 21 and 28, 45% and 27% of the animals tested positive for the anti-DEA 1 antibody, through crossmatching and flow cytometry, respectively. Detecting antibodies using the flow cytometric technique has high specificity and sensitivity, while crossmatching methods are highly sensitive but manifest low specificity. Following blood transfusion, animals that did not present as positive through crossmatching or flow cytometry were considered different from all other DEA 1-positive blood groups.http://www.revistas.usp.br/bjvras/article/view/122274AlloantibodiesCanine antibodiesDEA 1Blood transfusion in dogs |
| spellingShingle | Suzana Claudia Spinola dos Santos Ludmila Rodrigues Moroz Mariane Melo dos Santos Allan Souza dos Santos Soraya Castro Trindade Roberto Meyer Maria de Fátima Dias Costa Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion Brazilian Journal of Veterinary Research and Animal Science Alloantibodies Canine antibodies DEA 1 Blood transfusion in dogs |
| title | Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion |
| title_full | Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion |
| title_fullStr | Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion |
| title_full_unstemmed | Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion |
| title_short | Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion |
| title_sort | detection of canine anti dea 1 antibodies using flow cytometry in dogs following dea 1 positive blood transfusion |
| topic | Alloantibodies Canine antibodies DEA 1 Blood transfusion in dogs |
| url | http://www.revistas.usp.br/bjvras/article/view/122274 |
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