Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer; Part 3: Selective Cytotoxic Activity of 22 Plants against Human Cancer Cell Lines
Medicinal plants have been traditionally used to treat cancer in Ethiopia. However, very few studies have reported the in vitro anticancer activities of medicinal plants that are collected from different agro-ecological zones of Ethiopia. Hence, the main aim of this study was to screen the cytotoxic...
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MDPI AG
2021-06-01
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| author | Solomon Tesfaye Hannah Braun Kaleab Asres Ephrem Engidawork Anteneh Belete Ilias Muhammad Christian Schulze Nadin Schultze Sebastian Guenther Patrick J. Bednarski |
| author_facet | Solomon Tesfaye Hannah Braun Kaleab Asres Ephrem Engidawork Anteneh Belete Ilias Muhammad Christian Schulze Nadin Schultze Sebastian Guenther Patrick J. Bednarski |
| author_sort | Solomon Tesfaye |
| collection | DOAJ |
| description | Medicinal plants have been traditionally used to treat cancer in Ethiopia. However, very few studies have reported the in vitro anticancer activities of medicinal plants that are collected from different agro-ecological zones of Ethiopia. Hence, the main aim of this study was to screen the cytotoxic activities of 80% methanol extracts of 22 plants against human peripheral blood mononuclear cells (PBMCs), as well as human breast (MCF-7), lung (A427), bladder (RT-4), and cervical (SiSo) cancer cell lines. Active extracts were further screened against human large cell lung carcinoma (LCLC-103H), pancreatic cancer (DAN-G), ovarian cancer (A2780), and squamous cell carcinoma of the esophagus (KYSE-70) by using the crystal violet cell proliferation assay, while the vitality of the acute myeloid leukemia (HL-60) and histiocytic lymphoma (U-937) cell lines was monitored in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2<i>H</i>-tetrazolium bromide (MTT) microtiter assay. <i>Euphorbia schimperiana</i>, <i>Acokanthera schimperi</i>, <i>Kniphofia foliosa</i>, and <i>Kalanchoe petitiana</i> exhibited potent antiproliferative activity against A427, RT-4, MCF-7, and SiSo cell lines, with IC<sub>50</sub> values ranging from 1.85 ± 0.44 to 17.8 ± 2.31 µg/mL. Furthermore, these four extracts also showed potent antiproliferative activities against LCLC-103H, DAN-G, A2780, KYSE-70, HL-60, and U-937 cell lines, with IC<sub>50</sub> values ranging from 0.086 to 27.06 ± 10.8 µg/mL. Hence, further studies focusing on bio-assay-guided isolation and structural elucidation of active cytotoxic compounds from these plants are warranted. |
| first_indexed | 2024-03-10T10:22:56Z |
| format | Article |
| id | doaj.art-e7c6a93c13864d48b075b7d57ff5af5a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:22:56Z |
| publishDate | 2021-06-01 |
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| spelling | doaj.art-e7c6a93c13864d48b075b7d57ff5af5a2023-11-22T00:16:03ZengMDPI AGMolecules1420-30492021-06-012612365810.3390/molecules26123658Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer; Part 3: Selective Cytotoxic Activity of 22 Plants against Human Cancer Cell LinesSolomon Tesfaye0Hannah Braun1Kaleab Asres2Ephrem Engidawork3Anteneh Belete4Ilias Muhammad5Christian Schulze6Nadin Schultze7Sebastian Guenther8Patrick J. Bednarski9School of Pharmacy, College of Health Sciences, Addis Ababa University, Churchill Street, Addis Ababa 1176, EthiopiaDepartment of Pharmaceutical Biology, Institute of Pharmacy, University of Greifswald, 17489 Greifswald, GermanySchool of Pharmacy, College of Health Sciences, Addis Ababa University, Churchill Street, Addis Ababa 1176, EthiopiaSchool of Pharmacy, College of Health Sciences, Addis Ababa University, Churchill Street, Addis Ababa 1176, EthiopiaSchool of Pharmacy, College of Health Sciences, Addis Ababa University, Churchill Street, Addis Ababa 1176, EthiopiaNational Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USADepartment of Pharmaceutical Biology, Institute of Pharmacy, University of Greifswald, 17489 Greifswald, GermanyDepartment of Pharmaceutical Biology, Institute of Pharmacy, University of Greifswald, 17489 Greifswald, GermanyDepartment of Pharmaceutical Biology, Institute of Pharmacy, University of Greifswald, 17489 Greifswald, GermanyDepartment of Medicinal Chemistry, Institute of Pharmacy, University of Greifswald, 17489 Greifswald, GermanyMedicinal plants have been traditionally used to treat cancer in Ethiopia. However, very few studies have reported the in vitro anticancer activities of medicinal plants that are collected from different agro-ecological zones of Ethiopia. Hence, the main aim of this study was to screen the cytotoxic activities of 80% methanol extracts of 22 plants against human peripheral blood mononuclear cells (PBMCs), as well as human breast (MCF-7), lung (A427), bladder (RT-4), and cervical (SiSo) cancer cell lines. Active extracts were further screened against human large cell lung carcinoma (LCLC-103H), pancreatic cancer (DAN-G), ovarian cancer (A2780), and squamous cell carcinoma of the esophagus (KYSE-70) by using the crystal violet cell proliferation assay, while the vitality of the acute myeloid leukemia (HL-60) and histiocytic lymphoma (U-937) cell lines was monitored in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2<i>H</i>-tetrazolium bromide (MTT) microtiter assay. <i>Euphorbia schimperiana</i>, <i>Acokanthera schimperi</i>, <i>Kniphofia foliosa</i>, and <i>Kalanchoe petitiana</i> exhibited potent antiproliferative activity against A427, RT-4, MCF-7, and SiSo cell lines, with IC<sub>50</sub> values ranging from 1.85 ± 0.44 to 17.8 ± 2.31 µg/mL. Furthermore, these four extracts also showed potent antiproliferative activities against LCLC-103H, DAN-G, A2780, KYSE-70, HL-60, and U-937 cell lines, with IC<sub>50</sub> values ranging from 0.086 to 27.06 ± 10.8 µg/mL. Hence, further studies focusing on bio-assay-guided isolation and structural elucidation of active cytotoxic compounds from these plants are warranted.https://www.mdpi.com/1420-3049/26/12/3658Ethiopiamedicinal plantscytotoxicityextractionscancer |
| spellingShingle | Solomon Tesfaye Hannah Braun Kaleab Asres Ephrem Engidawork Anteneh Belete Ilias Muhammad Christian Schulze Nadin Schultze Sebastian Guenther Patrick J. Bednarski Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer; Part 3: Selective Cytotoxic Activity of 22 Plants against Human Cancer Cell Lines Molecules Ethiopia medicinal plants cytotoxicity extractions cancer |
| title | Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer; Part 3: Selective Cytotoxic Activity of 22 Plants against Human Cancer Cell Lines |
| title_full | Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer; Part 3: Selective Cytotoxic Activity of 22 Plants against Human Cancer Cell Lines |
| title_fullStr | Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer; Part 3: Selective Cytotoxic Activity of 22 Plants against Human Cancer Cell Lines |
| title_full_unstemmed | Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer; Part 3: Selective Cytotoxic Activity of 22 Plants against Human Cancer Cell Lines |
| title_short | Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer; Part 3: Selective Cytotoxic Activity of 22 Plants against Human Cancer Cell Lines |
| title_sort | ethiopian medicinal plants traditionally used for the treatment of cancer part 3 selective cytotoxic activity of 22 plants against human cancer cell lines |
| topic | Ethiopia medicinal plants cytotoxicity extractions cancer |
| url | https://www.mdpi.com/1420-3049/26/12/3658 |
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