Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications
Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The reson...
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| Format: | Article |
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MDPI AG
2014-03-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/14/3/4585 |
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| author | Amir Heidari Yong-Jin Yoon Woo-Tae Park Pei-Chen Su Jianmin Miao Julius Tsai Ming Lin Mi Kyoung Park |
| author_facet | Amir Heidari Yong-Jin Yoon Woo-Tae Park Pei-Chen Su Jianmin Miao Julius Tsai Ming Lin Mi Kyoung Park |
| author_sort | Amir Heidari |
| collection | DOAJ |
| description | Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests. |
| first_indexed | 2024-12-10T06:57:01Z |
| format | Article |
| id | doaj.art-e7c7814ac1cb45338c4a86a6cb4ec66a |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T06:57:01Z |
| publishDate | 2014-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-e7c7814ac1cb45338c4a86a6cb4ec66a2022-12-22T01:58:25ZengMDPI AGSensors1424-82202014-03-011434585459810.3390/s140304585s140304585Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor ApplicationsAmir Heidari0Yong-Jin Yoon1Woo-Tae Park2Pei-Chen Su3Jianmin Miao4Julius Tsai Ming Lin5Mi Kyoung Park6Institute of Microelectronics, Agency for Science, Technology and Research (A*STAR), Singapore 117685, SingaporeDepartment of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, SingaporeDepartment of Mechanical and Automotive Engineering, Seoul National University of Science and Technology, Seoul 139743, KoreaDepartment of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, SingaporeDepartment of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, SingaporeInstitute of Microelectronics, Agency for Science, Technology and Research (A*STAR), Singapore 117685, SingaporeInstitute of Microelectronics, Agency for Science, Technology and Research (A*STAR), Singapore 117685, SingaporeSensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests.http://www.mdpi.com/1424-8220/14/3/4585biosensorcapacitive filled gap transducerbiotinstreptavidin |
| spellingShingle | Amir Heidari Yong-Jin Yoon Woo-Tae Park Pei-Chen Su Jianmin Miao Julius Tsai Ming Lin Mi Kyoung Park Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications Sensors biosensor capacitive filled gap transducer biotin streptavidin |
| title | Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications |
| title_full | Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications |
| title_fullStr | Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications |
| title_full_unstemmed | Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications |
| title_short | Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications |
| title_sort | biotin streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label free biochemical sensor applications |
| topic | biosensor capacitive filled gap transducer biotin streptavidin |
| url | http://www.mdpi.com/1424-8220/14/3/4585 |
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