<i>Aeromonas dhakensis</i>: Clinical Isolates with High Carbapenem Resistance
<i>Aeromonas dhakensis</i> is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical (<i>n<...
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MDPI AG
2022-07-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/8/833 |
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| author | Suat Moi Puah Wei Ching Khor Kyaw Thu Aung Tien Tien Vicky Lau S. D. Puthucheary Kek Heng Chua |
| author_facet | Suat Moi Puah Wei Ching Khor Kyaw Thu Aung Tien Tien Vicky Lau S. D. Puthucheary Kek Heng Chua |
| author_sort | Suat Moi Puah |
| collection | DOAJ |
| description | <i>Aeromonas dhakensis</i> is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical (<i>n</i> = 94) and non-clinical (<i>n</i> = 23) isolates with dehydrated MicroScan microdilution. Carbapenem resistant isolates were further screened for genes related to carbapenem resistance using molecular assay. The isolates exhibited resistance to imipenem (76.9%), doripenem (62.4%), meropenem (41.9%), trimethoprim/sulfamethoxazole (11.1%), cefotaxime (8.5%), ceftazidime (6%), cefepime (1.7%) and aztreonam (0.9%), whereas all isolates were susceptible to amikacin. Clinical isolates showed significant association with resistance to doripenem, imipenem and meropenem compared to non-clinical isolates. These <i>bla</i><sub>cphA</sub> were detected in clinical isolates with resistance phenotypes: doripenem (67.2%, 45/67), imipenem (65.9%, 54/82) and meropenem (65.2%, 30/46). Our findings showed that the MicroScan microdilution method is suitable for the detection of carbapenem resistance in both clinical (48.9–87.2%) and non-clinical (4.3–13.0%) isolates. This study revealed that <i>A. dhakensis</i> isolates had relatively high carbapenem resistance, which may lead to potential treatment failure. Continued monitoring of aquatic sources with a larger sample size should be carried out to provide further insights. |
| first_indexed | 2024-03-09T09:51:23Z |
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| language | English |
| last_indexed | 2024-03-09T09:51:23Z |
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| spelling | doaj.art-e7ce5e35de18447fba5ee0cd967484172023-12-02T00:07:52ZengMDPI AGPathogens2076-08172022-07-0111883310.3390/pathogens11080833<i>Aeromonas dhakensis</i>: Clinical Isolates with High Carbapenem ResistanceSuat Moi Puah0Wei Ching Khor1Kyaw Thu Aung2Tien Tien Vicky Lau3S. D. Puthucheary4Kek Heng Chua5Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, MalaysiaNational Centre for Food Science, Singapore Food Agency, 52 Jurong Gateway Road, JEM Office Tower, 14-01, Singapore 608550, SingaporeNational Centre for Food Science, Singapore Food Agency, 52 Jurong Gateway Road, JEM Office Tower, 14-01, Singapore 608550, SingaporeDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia<i>Aeromonas dhakensis</i> is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical (<i>n</i> = 94) and non-clinical (<i>n</i> = 23) isolates with dehydrated MicroScan microdilution. Carbapenem resistant isolates were further screened for genes related to carbapenem resistance using molecular assay. The isolates exhibited resistance to imipenem (76.9%), doripenem (62.4%), meropenem (41.9%), trimethoprim/sulfamethoxazole (11.1%), cefotaxime (8.5%), ceftazidime (6%), cefepime (1.7%) and aztreonam (0.9%), whereas all isolates were susceptible to amikacin. Clinical isolates showed significant association with resistance to doripenem, imipenem and meropenem compared to non-clinical isolates. These <i>bla</i><sub>cphA</sub> were detected in clinical isolates with resistance phenotypes: doripenem (67.2%, 45/67), imipenem (65.9%, 54/82) and meropenem (65.2%, 30/46). Our findings showed that the MicroScan microdilution method is suitable for the detection of carbapenem resistance in both clinical (48.9–87.2%) and non-clinical (4.3–13.0%) isolates. This study revealed that <i>A. dhakensis</i> isolates had relatively high carbapenem resistance, which may lead to potential treatment failure. Continued monitoring of aquatic sources with a larger sample size should be carried out to provide further insights.https://www.mdpi.com/2076-0817/11/8/833<i>Aeromonas dhakensis</i>carbapenemCLSIEUCASTMicroScan |
| spellingShingle | Suat Moi Puah Wei Ching Khor Kyaw Thu Aung Tien Tien Vicky Lau S. D. Puthucheary Kek Heng Chua <i>Aeromonas dhakensis</i>: Clinical Isolates with High Carbapenem Resistance Pathogens <i>Aeromonas dhakensis</i> carbapenem CLSI EUCAST MicroScan |
| title | <i>Aeromonas dhakensis</i>: Clinical Isolates with High Carbapenem Resistance |
| title_full | <i>Aeromonas dhakensis</i>: Clinical Isolates with High Carbapenem Resistance |
| title_fullStr | <i>Aeromonas dhakensis</i>: Clinical Isolates with High Carbapenem Resistance |
| title_full_unstemmed | <i>Aeromonas dhakensis</i>: Clinical Isolates with High Carbapenem Resistance |
| title_short | <i>Aeromonas dhakensis</i>: Clinical Isolates with High Carbapenem Resistance |
| title_sort | i aeromonas dhakensis i clinical isolates with high carbapenem resistance |
| topic | <i>Aeromonas dhakensis</i> carbapenem CLSI EUCAST MicroScan |
| url | https://www.mdpi.com/2076-0817/11/8/833 |
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