The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis
Alshaymaa M Alhabibi,1 Maisa A Wahab,2 Ahmed Khairy Sakr,2 Samar M Abd El-Hamid,1 Mohamed Yahia Zakaria,3 Azza Ali Althoqapy,4 Hanaa Mohammed Eid El Sayed,5 Sammar Ahmed Kasim,5 Hanan F Ibrahim,4 Ola I Saleh,6 Howida A Ahmed,6 Alaa H Sayed,7 Mahmoud Lotfy8 1Department of Clinical Pathology, Faculty...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-11-01
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| Series: | Vascular Health and Risk Management |
| Subjects: | |
| Online Access: | https://www.dovepress.com/the-diagnostic-utility-of-natural-killer-cell-subsets-in-deep-vein-thr-peer-reviewed-fulltext-article-VHRM |
| _version_ | 1827659968751337472 |
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| author | Alhabibi AM Wahab MA Sakr AK Abd El-Hamid SM Zakaria MY Althoqapy AA El Sayed HME Kasim SA Ibrahim HF Saleh OI Ahmed HA Sayed AH Lotfy M |
| author_facet | Alhabibi AM Wahab MA Sakr AK Abd El-Hamid SM Zakaria MY Althoqapy AA El Sayed HME Kasim SA Ibrahim HF Saleh OI Ahmed HA Sayed AH Lotfy M |
| author_sort | Alhabibi AM |
| collection | DOAJ |
| description | Alshaymaa M Alhabibi,1 Maisa A Wahab,2 Ahmed Khairy Sakr,2 Samar M Abd El-Hamid,1 Mohamed Yahia Zakaria,3 Azza Ali Althoqapy,4 Hanaa Mohammed Eid El Sayed,5 Sammar Ahmed Kasim,5 Hanan F Ibrahim,4 Ola I Saleh,6 Howida A Ahmed,6 Alaa H Sayed,7 Mahmoud Lotfy8 1Department of Clinical Pathology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 2Department of Vascular Surgery, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 3Department of Vascular and EndoVascular Surgery, Faculty of Medicine for Boys, Al-Azhar University, Cairo, Egypt; 4Medical Microbiology and Immunology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 5Internal Medicine Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 6Department of Radio-Diagnosis, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 7Hormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt; 8Molecular Biology Department, Genetic Engineering & Biotechnology Research Institute, University of Sadat City, Sadat City, Minufiya, EgyptCorrespondence: Alshaymaa M Alhabibi, Tel +201002894075, Email alshaymaa.alhabibi@yahoo.comBackground: Natural killer (NK) cells are important components of adaptive and innate immune responses. NK cell subsets have different functions and may play a role in vascular disorders. This study aimed to evaluate the proportions of NK cells and their subsets to determine whether they can be used as markers of venous thrombosis and to identify whether there was a link between NK cell proportion and citrullinated histone (H3) levels.Patients and Methods: This study included 100 participants divided into Group I (n=50, patients with deep venous thrombosis (DVT)) and Group II (n=50, age- and sex-matched healthy controls). Group I was further categorized into Group Ia (n=25, patients with acute DVT) and Group Ib (n=25, patients with chronic DVT). The proportions of NK cells and their subsets were evaluated by flow cytometry using CD3/CD16/CD56. The levels of citrullinated histones (H3) were estimated using enzyme-linked immunosorbent assay (ELISA).Results: Compared to the control group, DVT patients had a significantly lower proportion of (CD56 dim/CD16+) NK cells, a significantly higher proportion of (CD56-/CD16+) NK cells and a high level of citrullinated histone (H3).Conclusion: NK cell subsets and citrullinated histone (H3) could be used as markers for DVT and as targets for therapeutic drugs to inhibit the formation or progression of thrombosis.Keywords: natural killer cell, natural killer cell subsets, citrullinated histone, deep venous thrombosis |
| first_indexed | 2024-03-09T23:31:39Z |
| format | Article |
| id | doaj.art-e7cf5172418c4c45b998e793edee3469 |
| institution | Directory Open Access Journal |
| issn | 1178-2048 |
| language | English |
| last_indexed | 2024-03-09T23:31:39Z |
| publishDate | 2023-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Vascular Health and Risk Management |
| spelling | doaj.art-e7cf5172418c4c45b998e793edee34692023-11-23T17:07:56ZengDove Medical PressVascular Health and Risk Management1178-20482023-11-01Volume 1977978788457The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein ThrombosisAlhabibi AMWahab MASakr AKAbd El-Hamid SMZakaria MYAlthoqapy AAEl Sayed HMEKasim SAIbrahim HFSaleh OIAhmed HASayed AHLotfy MAlshaymaa M Alhabibi,1 Maisa A Wahab,2 Ahmed Khairy Sakr,2 Samar M Abd El-Hamid,1 Mohamed Yahia Zakaria,3 Azza Ali Althoqapy,4 Hanaa Mohammed Eid El Sayed,5 Sammar Ahmed Kasim,5 Hanan F Ibrahim,4 Ola I Saleh,6 Howida A Ahmed,6 Alaa H Sayed,7 Mahmoud Lotfy8 1Department of Clinical Pathology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 2Department of Vascular Surgery, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 3Department of Vascular and EndoVascular Surgery, Faculty of Medicine for Boys, Al-Azhar University, Cairo, Egypt; 4Medical Microbiology and Immunology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 5Internal Medicine Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 6Department of Radio-Diagnosis, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 7Hormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt; 8Molecular Biology Department, Genetic Engineering & Biotechnology Research Institute, University of Sadat City, Sadat City, Minufiya, EgyptCorrespondence: Alshaymaa M Alhabibi, Tel +201002894075, Email alshaymaa.alhabibi@yahoo.comBackground: Natural killer (NK) cells are important components of adaptive and innate immune responses. NK cell subsets have different functions and may play a role in vascular disorders. This study aimed to evaluate the proportions of NK cells and their subsets to determine whether they can be used as markers of venous thrombosis and to identify whether there was a link between NK cell proportion and citrullinated histone (H3) levels.Patients and Methods: This study included 100 participants divided into Group I (n=50, patients with deep venous thrombosis (DVT)) and Group II (n=50, age- and sex-matched healthy controls). Group I was further categorized into Group Ia (n=25, patients with acute DVT) and Group Ib (n=25, patients with chronic DVT). The proportions of NK cells and their subsets were evaluated by flow cytometry using CD3/CD16/CD56. The levels of citrullinated histones (H3) were estimated using enzyme-linked immunosorbent assay (ELISA).Results: Compared to the control group, DVT patients had a significantly lower proportion of (CD56 dim/CD16+) NK cells, a significantly higher proportion of (CD56-/CD16+) NK cells and a high level of citrullinated histone (H3).Conclusion: NK cell subsets and citrullinated histone (H3) could be used as markers for DVT and as targets for therapeutic drugs to inhibit the formation or progression of thrombosis.Keywords: natural killer cell, natural killer cell subsets, citrullinated histone, deep venous thrombosishttps://www.dovepress.com/the-diagnostic-utility-of-natural-killer-cell-subsets-in-deep-vein-thr-peer-reviewed-fulltext-article-VHRMnatural killer cellnatural killer cell subsetscitrullinated histonedeep venous thrombosis. |
| spellingShingle | Alhabibi AM Wahab MA Sakr AK Abd El-Hamid SM Zakaria MY Althoqapy AA El Sayed HME Kasim SA Ibrahim HF Saleh OI Ahmed HA Sayed AH Lotfy M The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis Vascular Health and Risk Management natural killer cell natural killer cell subsets citrullinated histone deep venous thrombosis. |
| title | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
| title_full | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
| title_fullStr | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
| title_full_unstemmed | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
| title_short | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
| title_sort | diagnostic utility of natural killer cell subsets in deep vein thrombosis |
| topic | natural killer cell natural killer cell subsets citrullinated histone deep venous thrombosis. |
| url | https://www.dovepress.com/the-diagnostic-utility-of-natural-killer-cell-subsets-in-deep-vein-thr-peer-reviewed-fulltext-article-VHRM |
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