Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages
The purpose of this study is to elucidate the anti-inflammatory effect of lobeglitazone (LOBE) in lipopolysaccharide (LPS)-induced bone-marrow derived macrophages (BMDMs). We induced nitric oxide (NO) production and pro-inflammatory gene expression through LPS treatment in BMDMs. The changes of NO r...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/10/1432 |
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| author | Dabin Jeong Wan-Kyu Ko Seong-Jun Kim Gong-Ho Han Daye Lee Seung-Hun Sheen Seil Sohn |
| author_facet | Dabin Jeong Wan-Kyu Ko Seong-Jun Kim Gong-Ho Han Daye Lee Seung-Hun Sheen Seil Sohn |
| author_sort | Dabin Jeong |
| collection | DOAJ |
| description | The purpose of this study is to elucidate the anti-inflammatory effect of lobeglitazone (LOBE) in lipopolysaccharide (LPS)-induced bone-marrow derived macrophages (BMDMs). We induced nitric oxide (NO) production and pro-inflammatory gene expression through LPS treatment in BMDMs. The changes of NO release and expression of pro-inflammatory mediators by LOBE were assessed via NO quantification assay and a real-time quantitative polymerase chain reaction (RT-qPCR), respectively. In addition, the regulatory effect of LOBE on activation of mitogen-activated protein kinase (MAPK) signaling pathway was investigated by measuring the phosphorylation state of extracellular regulatory protein (ERK) and c-Jun N-terminal kinase (JNK) proteins by Western blot. Our results show that LOBE significantly reduced LPS-induced NO production and pro-inflammatory gene expression of interleukin-1β (IL-1β), IL-6, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and monocyte chemoattractant protein-1 (MCP-1). Moreover, LOBE reduced phosphorylation levels of ERK and JNK of MAPK signaling pathway. In conclusion, LOBE exerts an anti-inflammatory effect in LPS-induced BMDMs by suppression of NO production and pro-inflammatory gene expression, and this effect is potentially through inhibition of the MARK signaling pathway. |
| first_indexed | 2024-03-10T06:42:52Z |
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| id | doaj.art-e7d1b5a870d24adf88d39a2754d4e677 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T06:42:52Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-e7d1b5a870d24adf88d39a2754d4e6772023-11-22T17:31:49ZengMDPI AGBiomedicines2227-90592021-10-01910143210.3390/biomedicines9101432Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived MacrophagesDabin Jeong0Wan-Kyu Ko1Seong-Jun Kim2Gong-Ho Han3Daye Lee4Seung-Hun Sheen5Seil Sohn6Department of Neurosurgery, CHA Bundang Medical Center, Seongnam-si 13496, KoreaDepartment of Neurosurgery, CHA Bundang Medical Center, Seongnam-si 13496, KoreaDepartment of Neurosurgery, CHA Bundang Medical Center, Seongnam-si 13496, KoreaDepartment of Neurosurgery, CHA Bundang Medical Center, Seongnam-si 13496, KoreaDepartment of Neurosurgery, CHA Bundang Medical Center, Seongnam-si 13496, KoreaDepartment of Neurosurgery, CHA Bundang Medical Center, Seongnam-si 13496, KoreaDepartment of Neurosurgery, CHA Bundang Medical Center, Seongnam-si 13496, KoreaThe purpose of this study is to elucidate the anti-inflammatory effect of lobeglitazone (LOBE) in lipopolysaccharide (LPS)-induced bone-marrow derived macrophages (BMDMs). We induced nitric oxide (NO) production and pro-inflammatory gene expression through LPS treatment in BMDMs. The changes of NO release and expression of pro-inflammatory mediators by LOBE were assessed via NO quantification assay and a real-time quantitative polymerase chain reaction (RT-qPCR), respectively. In addition, the regulatory effect of LOBE on activation of mitogen-activated protein kinase (MAPK) signaling pathway was investigated by measuring the phosphorylation state of extracellular regulatory protein (ERK) and c-Jun N-terminal kinase (JNK) proteins by Western blot. Our results show that LOBE significantly reduced LPS-induced NO production and pro-inflammatory gene expression of interleukin-1β (IL-1β), IL-6, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and monocyte chemoattractant protein-1 (MCP-1). Moreover, LOBE reduced phosphorylation levels of ERK and JNK of MAPK signaling pathway. In conclusion, LOBE exerts an anti-inflammatory effect in LPS-induced BMDMs by suppression of NO production and pro-inflammatory gene expression, and this effect is potentially through inhibition of the MARK signaling pathway.https://www.mdpi.com/2227-9059/9/10/1432bone-marrow derived macrophageslipopolysaccharidelobeglitazoneanti-inflammationmitogen-activated protein kinase |
| spellingShingle | Dabin Jeong Wan-Kyu Ko Seong-Jun Kim Gong-Ho Han Daye Lee Seung-Hun Sheen Seil Sohn Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages Biomedicines bone-marrow derived macrophages lipopolysaccharide lobeglitazone anti-inflammation mitogen-activated protein kinase |
| title | Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages |
| title_full | Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages |
| title_fullStr | Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages |
| title_full_unstemmed | Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages |
| title_short | Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages |
| title_sort | lobeglitazone exerts anti inflammatory effect in lipopolysaccharide induced bone marrow derived macrophages |
| topic | bone-marrow derived macrophages lipopolysaccharide lobeglitazone anti-inflammation mitogen-activated protein kinase |
| url | https://www.mdpi.com/2227-9059/9/10/1432 |
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