Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany
Abstract Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide distribution and has been reported to induce clinical disease of varying severity in infected cattle, ranging from asymptomatic infections to severe enteric or respiratory disease. In this stud...
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BMC
2022-05-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-022-01817-y |
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| author | Sonja T. Jesse Malgorzata Ciurkiewicz Ute Siesenop Ingo Spitzbarth A. D. M. E. Osterhaus Wolfgang Baumgärtner Martin Ludlow |
| author_facet | Sonja T. Jesse Malgorzata Ciurkiewicz Ute Siesenop Ingo Spitzbarth A. D. M. E. Osterhaus Wolfgang Baumgärtner Martin Ludlow |
| author_sort | Sonja T. Jesse |
| collection | DOAJ |
| description | Abstract Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide distribution and has been reported to induce clinical disease of varying severity in infected cattle, ranging from asymptomatic infections to severe enteric or respiratory disease. In this study, we used next-generation sequencing to obtain the first complete genome sequence of a European strain of BadV-7, from pooled spleen and liver tissue obtained from a deceased newborn Limousin calf. Histopathological analysis and electron microscopy showing systemic lesions in multiple organs with intranuclear amphophilic inclusions observed in endothelial cells in multiple peripheral tissues. Virus isolation was readily achieved from tissue homogenate using bovine esophagus cells (KOP-R), a strategy which should facilitate future in vitro or in vivo BAdV-7 studies. Phylogenetic analysis of available genome sequences of BAdV-7 showed that the newly identified strain groups most closely with a recent BAdV-7 strain, SD18-74, from the USA, confirming that this newly identified strain is a member of the Atadenovirus genus. The fiber gene was found to be highly conserved within BAdV-7 strains but was highly divergent in comparison to Ovine adenovirus 7 (OAdV-7) (39.56% aa sequence identity). Furthermore, we report a variable region of multiple tandem repeats between the coding regions of E4.1 and RH5 genes. In summary, the presented pathological and molecular characterization of this case suggests that further research into the worldwide molecular epidemiology and disease burden of BAdV-7 is warranted. |
| first_indexed | 2024-04-12T17:04:50Z |
| format | Article |
| id | doaj.art-e7d27c0fca764aaaaeb9cf2d96764d5d |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-12T17:04:50Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-e7d27c0fca764aaaaeb9cf2d96764d5d2022-12-22T03:23:59ZengBMCVirology Journal1743-422X2022-05-0119111210.1186/s12985-022-01817-yMolecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in GermanySonja T. Jesse0Malgorzata Ciurkiewicz1Ute Siesenop2Ingo Spitzbarth3A. D. M. E. Osterhaus4Wolfgang Baumgärtner5Martin Ludlow6Research Center Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine HannoverDepartment of Pathology, University of Veterinary Medicine HanoverDepartment of Microbiology, University of Veterinary Medicine HanoverDepartment of Pathology, University of Veterinary Medicine HanoverResearch Center Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine HannoverDepartment of Pathology, University of Veterinary Medicine HanoverResearch Center Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine HannoverAbstract Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide distribution and has been reported to induce clinical disease of varying severity in infected cattle, ranging from asymptomatic infections to severe enteric or respiratory disease. In this study, we used next-generation sequencing to obtain the first complete genome sequence of a European strain of BadV-7, from pooled spleen and liver tissue obtained from a deceased newborn Limousin calf. Histopathological analysis and electron microscopy showing systemic lesions in multiple organs with intranuclear amphophilic inclusions observed in endothelial cells in multiple peripheral tissues. Virus isolation was readily achieved from tissue homogenate using bovine esophagus cells (KOP-R), a strategy which should facilitate future in vitro or in vivo BAdV-7 studies. Phylogenetic analysis of available genome sequences of BAdV-7 showed that the newly identified strain groups most closely with a recent BAdV-7 strain, SD18-74, from the USA, confirming that this newly identified strain is a member of the Atadenovirus genus. The fiber gene was found to be highly conserved within BAdV-7 strains but was highly divergent in comparison to Ovine adenovirus 7 (OAdV-7) (39.56% aa sequence identity). Furthermore, we report a variable region of multiple tandem repeats between the coding regions of E4.1 and RH5 genes. In summary, the presented pathological and molecular characterization of this case suggests that further research into the worldwide molecular epidemiology and disease burden of BAdV-7 is warranted.https://doi.org/10.1186/s12985-022-01817-yAdenovirusBovine adenovirus type 7Bovine Atadenovirus FCalfFiber genePathology |
| spellingShingle | Sonja T. Jesse Malgorzata Ciurkiewicz Ute Siesenop Ingo Spitzbarth A. D. M. E. Osterhaus Wolfgang Baumgärtner Martin Ludlow Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany Virology Journal Adenovirus Bovine adenovirus type 7 Bovine Atadenovirus F Calf Fiber gene Pathology |
| title | Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany |
| title_full | Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany |
| title_fullStr | Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany |
| title_full_unstemmed | Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany |
| title_short | Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany |
| title_sort | molecular characterization of a bovine adenovirus type 7 bovine atadenovirus f strain isolated from a systemically infected calf in germany |
| topic | Adenovirus Bovine adenovirus type 7 Bovine Atadenovirus F Calf Fiber gene Pathology |
| url | https://doi.org/10.1186/s12985-022-01817-y |
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