The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli
Abstract Background The aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-017-1100-9 |
| _version_ | 1811238274793996288 |
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| author | Emina Ćudić Kristin Surmann Gianna Panasia Elke Hammer Sabine Hunke |
| author_facet | Emina Ćudić Kristin Surmann Gianna Panasia Elke Hammer Sabine Hunke |
| author_sort | Emina Ćudić |
| collection | DOAJ |
| description | Abstract Background The aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and protein abundance. The findings were interpreted in relation to the global proteome profile upon gentamicin treatment. Results We observed specific interaction between CpxA and ArcA upon treatment with the aminoglycoside gentamicin using Membrane-Strep-tagged protein interaction experiments (mSPINE). This interaction was neither accompanied by detectable phosphorylation of ArcA nor by activation of the Arc system via CpxA. Furthermore, no changes in absolute amounts of the Cpx- and Arc-TCS could be determined with the sensitive single reaction monitoring (SRM) in presence of gentamicin. Nevertheless, upon applying shotgun mass spectrometry analysis after treatment with gentamicin, we observed a reduction of ArcA ~ P-dependent protein synthesis and a significant Cpx-dependent alteration in the global proteome profile of E. coli. Conclusions This study points to the importance of the Cpx-TCS within the complex regulatory network in the E. coli response to aminoglycoside-caused stress. |
| first_indexed | 2024-04-12T12:38:56Z |
| format | Article |
| id | doaj.art-e7d2e6f6051549709ca5da191d346adf |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-12T12:38:56Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-e7d2e6f6051549709ca5da191d346adf2022-12-22T03:32:50ZengBMCBMC Microbiology1471-21802017-09-0117111710.1186/s12866-017-1100-9The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coliEmina ĆudićKristin SurmannGianna Panasia0Elke Hammer1Sabine Hunke2FB 5 Microbiology, Department of Biology/Chemistry, University OsnabrückDepartment of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine GreifswaldFB 5 Microbiology, Department of Biology/Chemistry, University OsnabrückAbstract Background The aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and protein abundance. The findings were interpreted in relation to the global proteome profile upon gentamicin treatment. Results We observed specific interaction between CpxA and ArcA upon treatment with the aminoglycoside gentamicin using Membrane-Strep-tagged protein interaction experiments (mSPINE). This interaction was neither accompanied by detectable phosphorylation of ArcA nor by activation of the Arc system via CpxA. Furthermore, no changes in absolute amounts of the Cpx- and Arc-TCS could be determined with the sensitive single reaction monitoring (SRM) in presence of gentamicin. Nevertheless, upon applying shotgun mass spectrometry analysis after treatment with gentamicin, we observed a reduction of ArcA ~ P-dependent protein synthesis and a significant Cpx-dependent alteration in the global proteome profile of E. coli. Conclusions This study points to the importance of the Cpx-TCS within the complex regulatory network in the E. coli response to aminoglycoside-caused stress.http://link.springer.com/article/10.1186/s12866-017-1100-9E. coliTwo - component systemCpxArcAminoglycosidesProtein interactions |
| spellingShingle | Emina Ćudić Kristin Surmann Gianna Panasia Elke Hammer Sabine Hunke The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli BMC Microbiology E. coli Two - component system Cpx Arc Aminoglycosides Protein interactions |
| title | The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli |
| title_full | The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli |
| title_fullStr | The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli |
| title_full_unstemmed | The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli |
| title_short | The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli |
| title_sort | role of the two component systems cpx and arc in protein alterations upon gentamicin treatment in escherichia coli |
| topic | E. coli Two - component system Cpx Arc Aminoglycosides Protein interactions |
| url | http://link.springer.com/article/10.1186/s12866-017-1100-9 |
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