Ring stage classification of Babesia microti and Plasmodium falciparum using optical diffraction 3D tomographic technique
Abstract Background Babesia is an intraerythrocytic parasite often misdiagnosed as a malaria parasite, leading to inappropriate treatment of the disease especially in co-endemic areas. In recent years, optical diffraction tomography (ODT) has shown great potential in the field of pathogen detection...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
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BMC
2022-11-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-022-05569-0 |
| _version_ | 1811189429028519936 |
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| author | Ernest Mazigo Hojong Jun Jeonghun Oh Wasiq Malik Johnsy Mary Louis Tong-Soo Kim Se Jin Lee Sunghun Na Wanjoo Chun Won Sun Park Yong-Keun Park Eun-Taek Han Min-Jae Kim Jin-Hee Han |
| author_facet | Ernest Mazigo Hojong Jun Jeonghun Oh Wasiq Malik Johnsy Mary Louis Tong-Soo Kim Se Jin Lee Sunghun Na Wanjoo Chun Won Sun Park Yong-Keun Park Eun-Taek Han Min-Jae Kim Jin-Hee Han |
| author_sort | Ernest Mazigo |
| collection | DOAJ |
| description | Abstract Background Babesia is an intraerythrocytic parasite often misdiagnosed as a malaria parasite, leading to inappropriate treatment of the disease especially in co-endemic areas. In recent years, optical diffraction tomography (ODT) has shown great potential in the field of pathogen detection by quantification of three-dimensional (3D) imaging tomograms. The 3D imaging of biological cells is crucial to investigate and provide valuable information about the mechanisms behind the pathophysiology of cells and tissues. Methods The early ring stage of P. falciparum were obtained from stored stock of infected RBCs and of B. microti were obtained from infected patients during diagnosis. The ODT technique was applied to analyze and characterize detailed differences between P. falciparum and B. microti ring stage at the single cell level. Based on 3D quantitative information, accurate measurement was performed of morphological, biochemical, and biophysical parameters. Results Accurate measurements of morphological parameters indicated that the host cell surface area at the ring stage in B. microti was significantly smaller (140.2 ± 17.1 µm2) than that in P. falciparum (159.0 ± 15.2 µm2), and sphericities showed higher levels in B. microti-parasitized cells (0.66 ± 0.05) than in P. falciparum (0.60 ± 0.04). Based on biochemical parameters, host cell hemoglobin level was significantly higher and membrane fluctuations were respectively more active in P. falciparum-infected cells (30.25 ± 2.96 pg; 141.3 ± 24.68 nm) than in B. microti (27.28 ± 3.52 pg; 110.1 ± 38.83 nm). The result indicates that P. falciparum more actively altered host RBCs than B. microti. Conclusion Although P. falciparum and B. microti often show confusable characteristics under the microscope, and the actual three-dimensional properties are different. These differences could be used in differential clinical diagnosis of erythrocytes infected with B. microti and P. falciparum. Graphical Abstract |
| first_indexed | 2024-04-11T14:35:45Z |
| format | Article |
| id | doaj.art-e7dbd9541cef4a0c828bbc59e7539059 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-11T14:35:45Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-e7dbd9541cef4a0c828bbc59e75390592022-12-22T04:18:21ZengBMCParasites & Vectors1756-33052022-11-0115111010.1186/s13071-022-05569-0Ring stage classification of Babesia microti and Plasmodium falciparum using optical diffraction 3D tomographic techniqueErnest Mazigo0Hojong Jun1Jeonghun Oh2Wasiq Malik3Johnsy Mary Louis4Tong-Soo Kim5Se Jin Lee6Sunghun Na7Wanjoo Chun8Won Sun Park9Yong-Keun Park10Eun-Taek Han11Min-Jae Kim12Jin-Hee Han13Department of Medical Environmental Biology and Tropical Medicine, Kangwon National University School of MedicineDepartment of Tropical Medicine, Inha University College of MedicineDepartment of Physics, Korea Advanced Institute of Science and Technology (KAIST)Department of Medical Environmental Biology and Tropical Medicine, Kangwon National University School of MedicineDepartment of Medical Environmental Biology and Tropical Medicine, Kangwon National University School of MedicineDepartment of Tropical Medicine, Inha University College of MedicineDepartment of Obstetrics and Gynecology, Kangwon National University Hospital, Kangwon National University School of MedicineDepartment of Obstetrics and Gynecology, Kangwon National University Hospital, Kangwon National University School of MedicineDepartment of Pharmacology, Kangwon National University School of MedicineDepartment of Physiology, Kangwon National University School of MedicineDepartment of Physics, Korea Advanced Institute of Science and Technology (KAIST)Department of Medical Environmental Biology and Tropical Medicine, Kangwon National University School of MedicineDepartment of Infectious Diseases, Asan Medical Center, University of Ulsan College of MedicineDepartment of Medical Environmental Biology and Tropical Medicine, Kangwon National University School of MedicineAbstract Background Babesia is an intraerythrocytic parasite often misdiagnosed as a malaria parasite, leading to inappropriate treatment of the disease especially in co-endemic areas. In recent years, optical diffraction tomography (ODT) has shown great potential in the field of pathogen detection by quantification of three-dimensional (3D) imaging tomograms. The 3D imaging of biological cells is crucial to investigate and provide valuable information about the mechanisms behind the pathophysiology of cells and tissues. Methods The early ring stage of P. falciparum were obtained from stored stock of infected RBCs and of B. microti were obtained from infected patients during diagnosis. The ODT technique was applied to analyze and characterize detailed differences between P. falciparum and B. microti ring stage at the single cell level. Based on 3D quantitative information, accurate measurement was performed of morphological, biochemical, and biophysical parameters. Results Accurate measurements of morphological parameters indicated that the host cell surface area at the ring stage in B. microti was significantly smaller (140.2 ± 17.1 µm2) than that in P. falciparum (159.0 ± 15.2 µm2), and sphericities showed higher levels in B. microti-parasitized cells (0.66 ± 0.05) than in P. falciparum (0.60 ± 0.04). Based on biochemical parameters, host cell hemoglobin level was significantly higher and membrane fluctuations were respectively more active in P. falciparum-infected cells (30.25 ± 2.96 pg; 141.3 ± 24.68 nm) than in B. microti (27.28 ± 3.52 pg; 110.1 ± 38.83 nm). The result indicates that P. falciparum more actively altered host RBCs than B. microti. Conclusion Although P. falciparum and B. microti often show confusable characteristics under the microscope, and the actual three-dimensional properties are different. These differences could be used in differential clinical diagnosis of erythrocytes infected with B. microti and P. falciparum. Graphical Abstracthttps://doi.org/10.1186/s13071-022-05569-0Red blood cellsOptical diffraction tomographyPlasmodium falciparumBabesia microtiThree-dimensional refractive index |
| spellingShingle | Ernest Mazigo Hojong Jun Jeonghun Oh Wasiq Malik Johnsy Mary Louis Tong-Soo Kim Se Jin Lee Sunghun Na Wanjoo Chun Won Sun Park Yong-Keun Park Eun-Taek Han Min-Jae Kim Jin-Hee Han Ring stage classification of Babesia microti and Plasmodium falciparum using optical diffraction 3D tomographic technique Parasites & Vectors Red blood cells Optical diffraction tomography Plasmodium falciparum Babesia microti Three-dimensional refractive index |
| title | Ring stage classification of Babesia microti and Plasmodium falciparum using optical diffraction 3D tomographic technique |
| title_full | Ring stage classification of Babesia microti and Plasmodium falciparum using optical diffraction 3D tomographic technique |
| title_fullStr | Ring stage classification of Babesia microti and Plasmodium falciparum using optical diffraction 3D tomographic technique |
| title_full_unstemmed | Ring stage classification of Babesia microti and Plasmodium falciparum using optical diffraction 3D tomographic technique |
| title_short | Ring stage classification of Babesia microti and Plasmodium falciparum using optical diffraction 3D tomographic technique |
| title_sort | ring stage classification of babesia microti and plasmodium falciparum using optical diffraction 3d tomographic technique |
| topic | Red blood cells Optical diffraction tomography Plasmodium falciparum Babesia microti Three-dimensional refractive index |
| url | https://doi.org/10.1186/s13071-022-05569-0 |
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