Tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosis
Abstract Background To study the role of microecology and metabolism in iatrogenic tracheal injury and cicatricial stenosis, we investigated the tracheal microbiome and metabolome in patients with tracheal stenosis after endotracheal intubation. Methods We collected 16 protected specimen brush (PSB)...
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BMC
2023-09-01
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| Series: | BMC Pulmonary Medicine |
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| Online Access: | https://doi.org/10.1186/s12890-023-02654-7 |
| _version_ | 1797454052584325120 |
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| author | Zeqin Fan Lihui Zhang Li Wei Xiaoxian Huang Mei Yang Xiqian Xing |
| author_facet | Zeqin Fan Lihui Zhang Li Wei Xiaoxian Huang Mei Yang Xiqian Xing |
| author_sort | Zeqin Fan |
| collection | DOAJ |
| description | Abstract Background To study the role of microecology and metabolism in iatrogenic tracheal injury and cicatricial stenosis, we investigated the tracheal microbiome and metabolome in patients with tracheal stenosis after endotracheal intubation. Methods We collected 16 protected specimen brush (PSB) and 8 broncho-alveolar lavage (BAL) samples from 8 iatrogenic subglottic tracheal stenosis patients, including 8 PSB samples from tracheal scar sites, 8 PSB samples from scar-free sites and 8 BAL samples, by lavaging the subsegmental bronchi of the right-middle lobe. Metagenomic sequencing was performed to characterize the microbiome profiling of 16 PSB and 8 BAL samples. Untargeted metabolomics was performed in 6 PSB samples (3 from tracheal scar PSB and 3 from tracheal scar-free PSB) using high-performance liquid chromatography‒mass spectrometry (LC‒MS). Results At the species level, the top four bacterial species were Neisseria subflava, Streptococcus oralis, Capnocytophaga gingivals, and Haemophilus aegyptius. The alpha and beta diversity among tracheal scar PSB, scar-free PSB and BAL samples were compared, and no significant differences were found. Untargeted metabolomics was performed in 6 PSB samples using LC‒MS, and only one statistically significant metabolite, carnitine, was identified. Pathway enrichment analysis of carnitine revealed significant enrichment in fatty acid oxidation. Conclusion Our study found that carnitine levels in tracheal scar tissue were significantly lower than those in scar-free tissue, which might be a new target for the prevention and treatment of iatrogenic tracheal stenosis in the future. |
| first_indexed | 2024-03-09T15:31:42Z |
| format | Article |
| id | doaj.art-e7dcfbf8f64d4aafa6452d3597c269d3 |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-03-09T15:31:42Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-e7dcfbf8f64d4aafa6452d3597c269d32023-11-26T12:13:27ZengBMCBMC Pulmonary Medicine1471-24662023-09-012311810.1186/s12890-023-02654-7Tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosisZeqin Fan0Lihui Zhang1Li Wei2Xiaoxian Huang3Mei Yang4Xiqian Xing5Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Yunnan UniversityDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Yunnan UniversityDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Yunnan UniversityDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Yunnan UniversityDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Yunnan UniversityDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Yunnan UniversityAbstract Background To study the role of microecology and metabolism in iatrogenic tracheal injury and cicatricial stenosis, we investigated the tracheal microbiome and metabolome in patients with tracheal stenosis after endotracheal intubation. Methods We collected 16 protected specimen brush (PSB) and 8 broncho-alveolar lavage (BAL) samples from 8 iatrogenic subglottic tracheal stenosis patients, including 8 PSB samples from tracheal scar sites, 8 PSB samples from scar-free sites and 8 BAL samples, by lavaging the subsegmental bronchi of the right-middle lobe. Metagenomic sequencing was performed to characterize the microbiome profiling of 16 PSB and 8 BAL samples. Untargeted metabolomics was performed in 6 PSB samples (3 from tracheal scar PSB and 3 from tracheal scar-free PSB) using high-performance liquid chromatography‒mass spectrometry (LC‒MS). Results At the species level, the top four bacterial species were Neisseria subflava, Streptococcus oralis, Capnocytophaga gingivals, and Haemophilus aegyptius. The alpha and beta diversity among tracheal scar PSB, scar-free PSB and BAL samples were compared, and no significant differences were found. Untargeted metabolomics was performed in 6 PSB samples using LC‒MS, and only one statistically significant metabolite, carnitine, was identified. Pathway enrichment analysis of carnitine revealed significant enrichment in fatty acid oxidation. Conclusion Our study found that carnitine levels in tracheal scar tissue were significantly lower than those in scar-free tissue, which might be a new target for the prevention and treatment of iatrogenic tracheal stenosis in the future.https://doi.org/10.1186/s12890-023-02654-7Subglottic tracheal stenosisTracheal microbiomeMetabolome |
| spellingShingle | Zeqin Fan Lihui Zhang Li Wei Xiaoxian Huang Mei Yang Xiqian Xing Tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosis BMC Pulmonary Medicine Subglottic tracheal stenosis Tracheal microbiome Metabolome |
| title | Tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosis |
| title_full | Tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosis |
| title_fullStr | Tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosis |
| title_full_unstemmed | Tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosis |
| title_short | Tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosis |
| title_sort | tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosis |
| topic | Subglottic tracheal stenosis Tracheal microbiome Metabolome |
| url | https://doi.org/10.1186/s12890-023-02654-7 |
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