Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli
A member of the attaching and effacing (AE) family of pathogens, enterohemorrhagic Escherichia coli (EHEC) induces dramatic changes to the intestinal cell cytoskeleton, including effacement of microvilli. Effacement by the related pathogen enteropathogenic E. coli (EPEC) requires the activity of the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2011-11-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00222/full |
| _version_ | 1828849653557231616 |
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| author | YuShuan eLai Kathleen eRiley Andrew eCai Andrew eCai John M Leong Ira M. Herman |
| author_facet | YuShuan eLai Kathleen eRiley Andrew eCai Andrew eCai John M Leong Ira M. Herman |
| author_sort | YuShuan eLai |
| collection | DOAJ |
| description | A member of the attaching and effacing (AE) family of pathogens, enterohemorrhagic Escherichia coli (EHEC) induces dramatic changes to the intestinal cell cytoskeleton, including effacement of microvilli. Effacement by the related pathogen enteropathogenic E. coli (EPEC) requires the activity of the Ca+2-dependent host protease, calpain, which participates in a variety of cellular processes, including cell adhesion and motility. We found that EHEC infection results in an increase in epithelial (CaCo-2a) cell calpain activity and that EHEC-induced microvillar effacement was blocked by ectopic expression of calpastatin, an endogenous calpain inhibitor, or by pretreatment of intestinal cells with a cell-penetrating version of calpastatin. In addition, ezrin, a known calpain substrate that links the plasma membrane to axial actin filaments in microvilli, was cleaved in a calpain-dependent manner during EHEC infection and lost from its normal locale within microvilli. Calpain may be a central conduit through which EHEC and other AE pathogens induce enterocyte cytoskeletal remodeling and exert their pathogenic effects. |
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| format | Article |
| id | doaj.art-e7dd9cbc8a164f8e99c0afe69bd7a821 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T22:56:09Z |
| publishDate | 2011-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e7dd9cbc8a164f8e99c0afe69bd7a8212022-12-22T00:08:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2011-11-01210.3389/fmicb.2011.0022213003Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coliYuShuan eLai0Kathleen eRiley1Andrew eCai2Andrew eCai3John M Leong4Ira M. Herman5University of Massachusetts Medical SchoolTufts UniversityUniversity of Massachusetts Medical SchoolTufts UniversityUniversity of Massachusetts Medical SchoolTufts UniversityA member of the attaching and effacing (AE) family of pathogens, enterohemorrhagic Escherichia coli (EHEC) induces dramatic changes to the intestinal cell cytoskeleton, including effacement of microvilli. Effacement by the related pathogen enteropathogenic E. coli (EPEC) requires the activity of the Ca+2-dependent host protease, calpain, which participates in a variety of cellular processes, including cell adhesion and motility. We found that EHEC infection results in an increase in epithelial (CaCo-2a) cell calpain activity and that EHEC-induced microvillar effacement was blocked by ectopic expression of calpastatin, an endogenous calpain inhibitor, or by pretreatment of intestinal cells with a cell-penetrating version of calpastatin. In addition, ezrin, a known calpain substrate that links the plasma membrane to axial actin filaments in microvilli, was cleaved in a calpain-dependent manner during EHEC infection and lost from its normal locale within microvilli. Calpain may be a central conduit through which EHEC and other AE pathogens induce enterocyte cytoskeletal remodeling and exert their pathogenic effects.http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00222/fullMicrovilliattaching and effacing (AE) lesionCaCo-2CalpastatcalpastatinEzrin |
| spellingShingle | YuShuan eLai Kathleen eRiley Andrew eCai Andrew eCai John M Leong Ira M. Herman Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli Frontiers in Microbiology Microvilli attaching and effacing (AE) lesion CaCo-2 Calpastat calpastatin Ezrin |
| title | Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli |
| title_full | Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli |
| title_fullStr | Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli |
| title_full_unstemmed | Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli |
| title_short | Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli |
| title_sort | calpain mediates epithelial cell microvillar effacement by enterohemorrhagic escherichia coli |
| topic | Microvilli attaching and effacing (AE) lesion CaCo-2 Calpastat calpastatin Ezrin |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00222/full |
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