Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases
Objective To establish a simple and rapid qualitative and quantitative detection method of dexmedetomidine in blood. Methods Blood was separated on the Allure PFP Propyl liquid chromatography column with isocratic elution after it was precipitated by acetonitrile and filtered. Qualitative and quanti...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Forensic Medicine
2023-10-01
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| Series: | Fayixue Zazhi |
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| Online Access: | http://www.fyxzz.cn/fileup/1004-5619/PDF/2022.320306.pdf |
| _version_ | 1797347990091857920 |
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| author | Wen-ya ZHAI, Li-na WU, Shuo YANG, Bao-hua SHEN, Yan SHI |
| author_facet | Wen-ya ZHAI, Li-na WU, Shuo YANG, Bao-hua SHEN, Yan SHI |
| author_sort | Wen-ya ZHAI, Li-na WU, Shuo YANG, Bao-hua SHEN, Yan SHI |
| collection | DOAJ |
| description | Objective To establish a simple and rapid qualitative and quantitative detection method of dexmedetomidine in blood. Methods Blood was separated on the Allure PFP Propyl liquid chromatography column with isocratic elution after it was precipitated by acetonitrile and filtered. Qualitative and quantitative analysis of dexmedetomidine was performed using positive ion scan mode and multi-reaction monitoring mode. Results The limit of detection of dexmedetomidine in blood was 0.2 ng/mL and the limit of quantification was 0.5 ng/mL. The linearity of the method was good in the range of 0.5-1 000 ng/mL, and the correlation coefficient was greater than 0.99. The accuracy of the method was 90.34%-112.67% and the extraction recovery was 50.05%-91.08%, with no significant matrix effect. Conclusion This method is simple, selective and suitable for the qualitative and quantitative analysis of dexmedetomidine in blood, which can provide a reference for drug-facilitated cases involving dexmedetomidine. |
| first_indexed | 2024-03-08T11:56:54Z |
| format | Article |
| id | doaj.art-e7ddc456600243b08ac2bebf8d6778d1 |
| institution | Directory Open Access Journal |
| issn | 1004-5619 |
| language | zho |
| last_indexed | 2024-03-08T11:56:54Z |
| publishDate | 2023-10-01 |
| publisher | Editorial Office of Journal of Forensic Medicine |
| record_format | Article |
| series | Fayixue Zazhi |
| spelling | doaj.art-e7ddc456600243b08ac2bebf8d6778d12024-01-24T02:19:30ZzhoEditorial Office of Journal of Forensic MedicineFayixue Zazhi1004-56192023-10-0139545245610.12116/j.issn.1004-5619.2022.320306Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated CasesWen-ya ZHAI, Li-na WU, Shuo YANG, Bao-hua SHEN, Yan SHI01.Shanghai Key Laboratory of Forensic Medicine，Key Laboratory of Forensic Science，Ministry of Justice，Shanghai Forensic Service Platform，Academy of Forensic Science，Shanghai 200063，China;2.School of Forensic Medicine，Xi’an Jiaotong University，Xi’an 710061，ChinaObjective To establish a simple and rapid qualitative and quantitative detection method of dexmedetomidine in blood. Methods Blood was separated on the Allure PFP Propyl liquid chromatography column with isocratic elution after it was precipitated by acetonitrile and filtered. Qualitative and quantitative analysis of dexmedetomidine was performed using positive ion scan mode and multi-reaction monitoring mode. Results The limit of detection of dexmedetomidine in blood was 0.2 ng/mL and the limit of quantification was 0.5 ng/mL. The linearity of the method was good in the range of 0.5-1 000 ng/mL, and the correlation coefficient was greater than 0.99. The accuracy of the method was 90.34%-112.67% and the extraction recovery was 50.05%-91.08%, with no significant matrix effect. Conclusion This method is simple, selective and suitable for the qualitative and quantitative analysis of dexmedetomidine in blood, which can provide a reference for drug-facilitated cases involving dexmedetomidine.http://www.fyxzz.cn/fileup/1004-5619/PDF/2022.320306.pdf|forensic toxicology|drug facilitated crimes|dexmedetomidine|liquid chromatography-tandem mass spectrometry|blood |
| spellingShingle | Wen-ya ZHAI, Li-na WU, Shuo YANG, Bao-hua SHEN, Yan SHI Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases Fayixue Zazhi |forensic toxicology|drug facilitated crimes|dexmedetomidine|liquid chromatography-tandem mass spectrometry|blood |
| title | Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases |
| title_full | Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases |
| title_fullStr | Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases |
| title_full_unstemmed | Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases |
| title_short | Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases |
| title_sort | detection and analysis of blood dexmedetomidine in drug facilitated cases |
| topic | |forensic toxicology|drug facilitated crimes|dexmedetomidine|liquid chromatography-tandem mass spectrometry|blood |
| url | http://www.fyxzz.cn/fileup/1004-5619/PDF/2022.320306.pdf |
| work_keys_str_mv | AT wenyazhailinawushuoyangbaohuashenyanshi detectionandanalysisofblooddexmedetomidineindrugfacilitatedcases |