B Cells Are Indispensable for a Novel Mouse Model of Primary Sjögren’s Syndrome
Primary Sjögren’s syndrome (pSS) is characterized by a panel of autoantibodies, while it is not clear whether B cells and autoantibodies play an essential role in pathogenesis of the disease. Here, we report a novel mouse model for pSS which is induced by immunization with the Ro60_316-335 peptide c...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01384/full |
| _version_ | 1811343785312911360 |
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| author | Junfeng Zheng Junfeng Zheng Qiaoniang Huang Renliang Huang Fengyuan Deng Xiaoyang Yue Junping Yin Wenjie Zhao Yan Chen Lifang Wen Jun Zhou Renda Huang Gabriela Riemekasten Gabriela Riemekasten Zuguo Liu Frank Petersen Xinhua Yu Xinhua Yu |
| author_facet | Junfeng Zheng Junfeng Zheng Qiaoniang Huang Renliang Huang Fengyuan Deng Xiaoyang Yue Junping Yin Wenjie Zhao Yan Chen Lifang Wen Jun Zhou Renda Huang Gabriela Riemekasten Gabriela Riemekasten Zuguo Liu Frank Petersen Xinhua Yu Xinhua Yu |
| author_sort | Junfeng Zheng |
| collection | DOAJ |
| description | Primary Sjögren’s syndrome (pSS) is characterized by a panel of autoantibodies, while it is not clear whether B cells and autoantibodies play an essential role in pathogenesis of the disease. Here, we report a novel mouse model for pSS which is induced by immunization with the Ro60_316-335 peptide containing a predominant T cell epitope. After immunization, mice developed several symptoms mimicking pSS, including a decreased secretion of tears, lymphocytic infiltration into the lacrimal glands, autoantibodies, and increased levels of inflammatory cytokines. Disease susceptibility to this novel mouse model varies among strains, where C3H/HeJ (H2-k) and C3H/HeN (H2-k) are susceptible while DBA/1 (H2-q) and C57BL/6 (H2-b) are resistant. Depletion of B cells using anti-CD20 monoclonal antibodies prevented C3H/HeN mice from development of the pSS-like disease. In addition, HLA-DRB1*0803, a pSS risk allele, was predicted to bind to the hRo60_308-328 which contains a predominant T cell epitope of human Ro60. Therefore, this study provides a novel mouse model for pSS and reveals an indispensable role of B cells in this model. Moreover, it suggests that T cell epitope within Ro60 antigen is potentially pathogenic for pSS. |
| first_indexed | 2024-04-13T19:36:05Z |
| format | Article |
| id | doaj.art-e7e059c0e5ee4a1eaee8f899cf57b03e |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T19:36:05Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-e7e059c0e5ee4a1eaee8f899cf57b03e2022-12-22T02:33:02ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-10-01810.3389/fimmu.2017.01384303942B Cells Are Indispensable for a Novel Mouse Model of Primary Sjögren’s SyndromeJunfeng Zheng0Junfeng Zheng1Qiaoniang Huang2Renliang Huang3Fengyuan Deng4Xiaoyang Yue5Junping Yin6Wenjie Zhao7Yan Chen8Lifang Wen9Jun Zhou10Renda Huang11Gabriela Riemekasten12Gabriela Riemekasten13Zuguo Liu14Frank Petersen15Xinhua Yu16Xinhua Yu17Xiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaInstitute of Psychiatry and Neuroscience, Xinxiang Medical University, XinXiang, ChinaXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaPriority Area Asthma & Allergy, Research Center Borstel, Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, GermanyXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaInstitute of Psychiatry and Neuroscience, Xinxiang Medical University, XinXiang, ChinaDepartment of Rheumatology, University of Lübeck, Lübeck, GermanyEye Institute of Xiamen University, The Medical College of Xiamen University, Xiamen, ChinaPriority Area Asthma & Allergy, Research Center Borstel, Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, GermanyXiamen-Borstel Joint Laboratory of Autoimmunity, Medical College of Xiamen University, Xiamen, ChinaPriority Area Asthma & Allergy, Research Center Borstel, Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, GermanyPrimary Sjögren’s syndrome (pSS) is characterized by a panel of autoantibodies, while it is not clear whether B cells and autoantibodies play an essential role in pathogenesis of the disease. Here, we report a novel mouse model for pSS which is induced by immunization with the Ro60_316-335 peptide containing a predominant T cell epitope. After immunization, mice developed several symptoms mimicking pSS, including a decreased secretion of tears, lymphocytic infiltration into the lacrimal glands, autoantibodies, and increased levels of inflammatory cytokines. Disease susceptibility to this novel mouse model varies among strains, where C3H/HeJ (H2-k) and C3H/HeN (H2-k) are susceptible while DBA/1 (H2-q) and C57BL/6 (H2-b) are resistant. Depletion of B cells using anti-CD20 monoclonal antibodies prevented C3H/HeN mice from development of the pSS-like disease. In addition, HLA-DRB1*0803, a pSS risk allele, was predicted to bind to the hRo60_308-328 which contains a predominant T cell epitope of human Ro60. Therefore, this study provides a novel mouse model for pSS and reveals an indispensable role of B cells in this model. Moreover, it suggests that T cell epitope within Ro60 antigen is potentially pathogenic for pSS.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01384/fullprimary Sjögren’s syndromemouse modelT cell epitopeSSAautoantibodiesB cells |
| spellingShingle | Junfeng Zheng Junfeng Zheng Qiaoniang Huang Renliang Huang Fengyuan Deng Xiaoyang Yue Junping Yin Wenjie Zhao Yan Chen Lifang Wen Jun Zhou Renda Huang Gabriela Riemekasten Gabriela Riemekasten Zuguo Liu Frank Petersen Xinhua Yu Xinhua Yu B Cells Are Indispensable for a Novel Mouse Model of Primary Sjögren’s Syndrome Frontiers in Immunology primary Sjögren’s syndrome mouse model T cell epitope SSA autoantibodies B cells |
| title | B Cells Are Indispensable for a Novel Mouse Model of Primary Sjögren’s Syndrome |
| title_full | B Cells Are Indispensable for a Novel Mouse Model of Primary Sjögren’s Syndrome |
| title_fullStr | B Cells Are Indispensable for a Novel Mouse Model of Primary Sjögren’s Syndrome |
| title_full_unstemmed | B Cells Are Indispensable for a Novel Mouse Model of Primary Sjögren’s Syndrome |
| title_short | B Cells Are Indispensable for a Novel Mouse Model of Primary Sjögren’s Syndrome |
| title_sort | b cells are indispensable for a novel mouse model of primary sjogren s syndrome |
| topic | primary Sjögren’s syndrome mouse model T cell epitope SSA autoantibodies B cells |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01384/full |
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