Serum Metabonomics Study of Difference in Lymph Node Metastasis of Pancreatic Ductal Adenocarcinoma Based on LC-MS
Objective To investigate the differential metabolites of lymph node metastasis in pancreatic ductal carcinoma (PDAC) and provide new ideas for the pathogenesis, early diagnosis and treatment of metastatic pancreatic cancer. Methods Forty serum specimens of patients with pancreatic ductal carcinoma w...
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| Format: | Article |
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Magazine House of Cancer Research on Prevention and Treatment
2023-10-01
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| Series: | Zhongliu Fangzhi Yanjiu |
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| Online Access: | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.23.0038 |
| _version_ | 1797635301555830784 |
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| author | HUANG Xiangping WU Ling TAN Chaochao |
| author_facet | HUANG Xiangping WU Ling TAN Chaochao |
| author_sort | HUANG Xiangping |
| collection | DOAJ |
| description | Objective To investigate the differential metabolites of lymph node metastasis in pancreatic ductal carcinoma (PDAC) and provide new ideas for the pathogenesis, early diagnosis and treatment of metastatic pancreatic cancer. Methods Forty serum specimens of patients with pancreatic ductal carcinoma were collected and divided into lymph node metastasis group (18 cases) and non-metastasis group (22 cases). Thirty-one serum specimens were also collected from the healthy control group. Liquid chromatographytandem mass spectrometry was used to analyze the differential metabolites and metabolic pathways between patients with PDAC and healthy controls as well as between lymph node metastasis and non-metastasis groups. Results Principal component analysis and partial least squares-discriminant analysis revealed statistically significant differences in metabolites and metabolic pathways between patients with PDAC and the healthy controls and between lymph node metastasis and non-metastasis groups. The differences in profiles were also statistically significant. Seventy-six different metabolites and 11 metabolic pathways were screened between patients with PDAC and the healthy controls, among which phenylalanine metabolism and histidine metabolism were the two most influential metabolic pathways. Four different metabolites were screened between lymph node metastasis and non-metastasis groups, and the expression of ethopropazine and phenylalanine were upregulated but the expression of tetrahydrodeoxycorticosterone and oxprenolol were downregulated. Conclusion Metabolites are significantly altered in the lymph node metastasis group of patients with PDAC compared with the non-metastasis group. Ethopropazine, phenylalanine, tetrahydrodeoxy corticosterone, and oxprenolol are potential biomarkers of lymph node metastasis in patients with PDAC. |
| first_indexed | 2024-03-11T12:20:18Z |
| format | Article |
| id | doaj.art-e7e20faee03347069819598d21f5546a |
| institution | Directory Open Access Journal |
| issn | 1000-8578 |
| language | zho |
| last_indexed | 2024-03-11T12:20:18Z |
| publishDate | 2023-10-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-e7e20faee03347069819598d21f5546a2023-11-07T05:14:03ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782023-10-01501097498010.3971/j.issn.1000-8578.2023.23.00388578.2023.23.0038Serum Metabonomics Study of Difference in Lymph Node Metastasis of Pancreatic Ductal Adenocarcinoma Based on LC-MSHUANG Xiangping0WU Ling1TAN Chaochao2Department of Laboratory Medicine, Hunan Provincial People's Hospital, the First Affiliated Hospital of Hunan Normal University, Changsha 410002, ChinaDepartment of Laboratory Medicine, Hunan Provincial People's Hospital, the First Affiliated Hospital of Hunan Normal University, Changsha 410002, ChinaDepartment of Laboratory Medicine, Hunan Provincial People's Hospital, the First Affiliated Hospital of Hunan Normal University, Changsha 410002, ChinaObjective To investigate the differential metabolites of lymph node metastasis in pancreatic ductal carcinoma (PDAC) and provide new ideas for the pathogenesis, early diagnosis and treatment of metastatic pancreatic cancer. Methods Forty serum specimens of patients with pancreatic ductal carcinoma were collected and divided into lymph node metastasis group (18 cases) and non-metastasis group (22 cases). Thirty-one serum specimens were also collected from the healthy control group. Liquid chromatographytandem mass spectrometry was used to analyze the differential metabolites and metabolic pathways between patients with PDAC and healthy controls as well as between lymph node metastasis and non-metastasis groups. Results Principal component analysis and partial least squares-discriminant analysis revealed statistically significant differences in metabolites and metabolic pathways between patients with PDAC and the healthy controls and between lymph node metastasis and non-metastasis groups. The differences in profiles were also statistically significant. Seventy-six different metabolites and 11 metabolic pathways were screened between patients with PDAC and the healthy controls, among which phenylalanine metabolism and histidine metabolism were the two most influential metabolic pathways. Four different metabolites were screened between lymph node metastasis and non-metastasis groups, and the expression of ethopropazine and phenylalanine were upregulated but the expression of tetrahydrodeoxycorticosterone and oxprenolol were downregulated. Conclusion Metabolites are significantly altered in the lymph node metastasis group of patients with PDAC compared with the non-metastasis group. Ethopropazine, phenylalanine, tetrahydrodeoxy corticosterone, and oxprenolol are potential biomarkers of lymph node metastasis in patients with PDAC.http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.23.0038liquid chromatography-tandem mass spectrometrypancreatic ductal adenocarcinomalymph node metastasismetabolomics |
| spellingShingle | HUANG Xiangping WU Ling TAN Chaochao Serum Metabonomics Study of Difference in Lymph Node Metastasis of Pancreatic Ductal Adenocarcinoma Based on LC-MS Zhongliu Fangzhi Yanjiu liquid chromatography-tandem mass spectrometry pancreatic ductal adenocarcinoma lymph node metastasis metabolomics |
| title | Serum Metabonomics Study of Difference in Lymph Node Metastasis of Pancreatic Ductal Adenocarcinoma Based on LC-MS |
| title_full | Serum Metabonomics Study of Difference in Lymph Node Metastasis of Pancreatic Ductal Adenocarcinoma Based on LC-MS |
| title_fullStr | Serum Metabonomics Study of Difference in Lymph Node Metastasis of Pancreatic Ductal Adenocarcinoma Based on LC-MS |
| title_full_unstemmed | Serum Metabonomics Study of Difference in Lymph Node Metastasis of Pancreatic Ductal Adenocarcinoma Based on LC-MS |
| title_short | Serum Metabonomics Study of Difference in Lymph Node Metastasis of Pancreatic Ductal Adenocarcinoma Based on LC-MS |
| title_sort | serum metabonomics study of difference in lymph node metastasis of pancreatic ductal adenocarcinoma based on lc ms |
| topic | liquid chromatography-tandem mass spectrometry pancreatic ductal adenocarcinoma lymph node metastasis metabolomics |
| url | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.23.0038 |
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