Distinct regions within the GluN2C subunit regulate the surface delivery of NMDA receptors
N-methyl-D-aspartate (NMDA) receptors mediate fast excitatory synaptic transmission in the mammalian central nervous system. The activation of NMDA receptors plays a key role in brain development, synaptic plasticity, and memory formation, and is a major contributor to many neuropsychiatric disorder...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-11-01
|
| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00375/full |
| _version_ | 1819144445902192640 |
|---|---|
| author | Katarina eLichnerova Katarina eLichnerova Martina eKaniakova Kristyna eSkrenkova Ladislav eVyklicky Martin eHorak |
| author_facet | Katarina eLichnerova Katarina eLichnerova Martina eKaniakova Kristyna eSkrenkova Ladislav eVyklicky Martin eHorak |
| author_sort | Katarina eLichnerova |
| collection | DOAJ |
| description | N-methyl-D-aspartate (NMDA) receptors mediate fast excitatory synaptic transmission in the mammalian central nervous system. The activation of NMDA receptors plays a key role in brain development, synaptic plasticity, and memory formation, and is a major contributor to many neuropsychiatric disorders. Here, we investigated the mechanisms that underlie the trafficking of GluN1/GluN2C receptors. Using an approach combining molecular biology, microscopy, and electrophysiology in mammalian cell lines and cultured cerebellar granule cells, we found that the surface delivery of GluN2C-containing receptors is reduced compared to GluN2A- and GluN2B-containing receptors. Furthermore, we identified three distinct regions within the N-terminus, M3 transmembrane domain, and C-terminus of GluN2C subunits that are required for proper intracellular processing and surface delivery of NMDA receptors. These results shed new light on the regulation of NMDA receptor trafficking, and these findings can be exploited to develop new strategies for treating some forms of neuropsychiatric disorders. |
| first_indexed | 2024-12-22T12:42:15Z |
| format | Article |
| id | doaj.art-e7e253aa33924e6cbba719a4ebceaf33 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-22T12:42:15Z |
| publishDate | 2014-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-e7e253aa33924e6cbba719a4ebceaf332022-12-21T18:25:25ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022014-11-01810.3389/fncel.2014.00375116568Distinct regions within the GluN2C subunit regulate the surface delivery of NMDA receptorsKatarina eLichnerova0Katarina eLichnerova1Martina eKaniakova2Kristyna eSkrenkova3Ladislav eVyklicky4Martin eHorak5Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i.Faculty of Science, Charles University in PragueInstitute of Physiology, Academy of Sciences of the Czech Republic v.v.i.Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i.Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i.Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i.N-methyl-D-aspartate (NMDA) receptors mediate fast excitatory synaptic transmission in the mammalian central nervous system. The activation of NMDA receptors plays a key role in brain development, synaptic plasticity, and memory formation, and is a major contributor to many neuropsychiatric disorders. Here, we investigated the mechanisms that underlie the trafficking of GluN1/GluN2C receptors. Using an approach combining molecular biology, microscopy, and electrophysiology in mammalian cell lines and cultured cerebellar granule cells, we found that the surface delivery of GluN2C-containing receptors is reduced compared to GluN2A- and GluN2B-containing receptors. Furthermore, we identified three distinct regions within the N-terminus, M3 transmembrane domain, and C-terminus of GluN2C subunits that are required for proper intracellular processing and surface delivery of NMDA receptors. These results shed new light on the regulation of NMDA receptor trafficking, and these findings can be exploited to develop new strategies for treating some forms of neuropsychiatric disorders.http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00375/fullElectrophysiologyion channelglutamate receptorintracellular traffickingcerebellar granule cellsendoplasmic reticulum. |
| spellingShingle | Katarina eLichnerova Katarina eLichnerova Martina eKaniakova Kristyna eSkrenkova Ladislav eVyklicky Martin eHorak Distinct regions within the GluN2C subunit regulate the surface delivery of NMDA receptors Frontiers in Cellular Neuroscience Electrophysiology ion channel glutamate receptor intracellular trafficking cerebellar granule cells endoplasmic reticulum. |
| title | Distinct regions within the GluN2C subunit regulate the surface delivery of NMDA receptors |
| title_full | Distinct regions within the GluN2C subunit regulate the surface delivery of NMDA receptors |
| title_fullStr | Distinct regions within the GluN2C subunit regulate the surface delivery of NMDA receptors |
| title_full_unstemmed | Distinct regions within the GluN2C subunit regulate the surface delivery of NMDA receptors |
| title_short | Distinct regions within the GluN2C subunit regulate the surface delivery of NMDA receptors |
| title_sort | distinct regions within the glun2c subunit regulate the surface delivery of nmda receptors |
| topic | Electrophysiology ion channel glutamate receptor intracellular trafficking cerebellar granule cells endoplasmic reticulum. |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00375/full |
| work_keys_str_mv | AT katarinaelichnerova distinctregionswithintheglun2csubunitregulatethesurfacedeliveryofnmdareceptors AT katarinaelichnerova distinctregionswithintheglun2csubunitregulatethesurfacedeliveryofnmdareceptors AT martinaekaniakova distinctregionswithintheglun2csubunitregulatethesurfacedeliveryofnmdareceptors AT kristynaeskrenkova distinctregionswithintheglun2csubunitregulatethesurfacedeliveryofnmdareceptors AT ladislavevyklicky distinctregionswithintheglun2csubunitregulatethesurfacedeliveryofnmdareceptors AT martinehorak distinctregionswithintheglun2csubunitregulatethesurfacedeliveryofnmdareceptors |