Single-cell Analysis Technologies for Immuno-oncology Research: from Mechanistic Delineation to Biomarker Discovery
The successes with immune checkpoint blockade (ICB) and chimeric antigen receptor (CAR)-T-cell therapy in treating multiple cancer types have established immunotherapy as a powerful curative option for patients with advanced cancers. Unfortunately, many patients do not derive benefit or long-term re...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-04-01
|
| Series: | Genomics, Proteomics & Bioinformatics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672022921000772 |
| _version_ | 1797368700122169344 |
|---|---|
| author | Zhiliang Bai Graham Su Rong Fan |
| author_facet | Zhiliang Bai Graham Su Rong Fan |
| author_sort | Zhiliang Bai |
| collection | DOAJ |
| description | The successes with immune checkpoint blockade (ICB) and chimeric antigen receptor (CAR)-T-cell therapy in treating multiple cancer types have established immunotherapy as a powerful curative option for patients with advanced cancers. Unfortunately, many patients do not derive benefit or long-term responses, highlighting a pressing need to perform complete investigation of the underlying mechanisms and the immunotherapy-induced tumor regression or rejection. In recent years, a large number of single-cell technologies have leveraged advances in characterizing immune system, profiling tumor microenvironment, and identifying cellular heterogeneity, which establish the foundations for lifting the veil on the comprehensive crosstalk between cancer and immune system during immunotherapies. In this review, we introduce the applications of the most widely used single-cell technologies in furthering our understanding of immunotherapies in terms of underlying mechanisms and their association with therapeutic outcomes. We also discuss how single-cell analyses help to deliver new insights into biomarker discovery to predict patient response rate, monitor acquired resistance, and support prophylactic strategy development for toxicity management. Finally, we provide an overview of applying cutting-edge single-cell spatial-omics to point out the heterogeneity of tumor–immune interactions at higher level that can ultimately guide to the rational design of next-generation immunotherapies. |
| first_indexed | 2024-03-08T17:36:22Z |
| format | Article |
| id | doaj.art-e7e7c7dbc16e406b86036798eb30d031 |
| institution | Directory Open Access Journal |
| issn | 1672-0229 |
| language | English |
| last_indexed | 2024-03-08T17:36:22Z |
| publishDate | 2021-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Genomics, Proteomics & Bioinformatics |
| spelling | doaj.art-e7e7c7dbc16e406b86036798eb30d0312024-01-02T12:23:06ZengElsevierGenomics, Proteomics & Bioinformatics1672-02292021-04-01192191207Single-cell Analysis Technologies for Immuno-oncology Research: from Mechanistic Delineation to Biomarker DiscoveryZhiliang Bai0Graham Su1Rong Fan2Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA; State Key Laboratory of Precision Measurement Technology and Instrument, Tianjin University, Tianjin 300072, ChinaDepartment of Biomedical Engineering, Yale University, New Haven, CT 06511, USADepartment of Biomedical Engineering, Yale University, New Haven, CT 06511, USA; Yale Stem Cell Center and Yale Cancer Center, Yale School of Medicine, New Haven, CT 06511, USA; Human and Translational Immunology, Yale School of Medicine, New Haven, CT 06511, USA; Corresponding author.The successes with immune checkpoint blockade (ICB) and chimeric antigen receptor (CAR)-T-cell therapy in treating multiple cancer types have established immunotherapy as a powerful curative option for patients with advanced cancers. Unfortunately, many patients do not derive benefit or long-term responses, highlighting a pressing need to perform complete investigation of the underlying mechanisms and the immunotherapy-induced tumor regression or rejection. In recent years, a large number of single-cell technologies have leveraged advances in characterizing immune system, profiling tumor microenvironment, and identifying cellular heterogeneity, which establish the foundations for lifting the veil on the comprehensive crosstalk between cancer and immune system during immunotherapies. In this review, we introduce the applications of the most widely used single-cell technologies in furthering our understanding of immunotherapies in terms of underlying mechanisms and their association with therapeutic outcomes. We also discuss how single-cell analyses help to deliver new insights into biomarker discovery to predict patient response rate, monitor acquired resistance, and support prophylactic strategy development for toxicity management. Finally, we provide an overview of applying cutting-edge single-cell spatial-omics to point out the heterogeneity of tumor–immune interactions at higher level that can ultimately guide to the rational design of next-generation immunotherapies.http://www.sciencedirect.com/science/article/pii/S1672022921000772Single-cell analysisImmunotherapyBiomarker discoveryCheckpoint blockadeCAR-T |
| spellingShingle | Zhiliang Bai Graham Su Rong Fan Single-cell Analysis Technologies for Immuno-oncology Research: from Mechanistic Delineation to Biomarker Discovery Genomics, Proteomics & Bioinformatics Single-cell analysis Immunotherapy Biomarker discovery Checkpoint blockade CAR-T |
| title | Single-cell Analysis Technologies for Immuno-oncology Research: from Mechanistic Delineation to Biomarker Discovery |
| title_full | Single-cell Analysis Technologies for Immuno-oncology Research: from Mechanistic Delineation to Biomarker Discovery |
| title_fullStr | Single-cell Analysis Technologies for Immuno-oncology Research: from Mechanistic Delineation to Biomarker Discovery |
| title_full_unstemmed | Single-cell Analysis Technologies for Immuno-oncology Research: from Mechanistic Delineation to Biomarker Discovery |
| title_short | Single-cell Analysis Technologies for Immuno-oncology Research: from Mechanistic Delineation to Biomarker Discovery |
| title_sort | single cell analysis technologies for immuno oncology research from mechanistic delineation to biomarker discovery |
| topic | Single-cell analysis Immunotherapy Biomarker discovery Checkpoint blockade CAR-T |
| url | http://www.sciencedirect.com/science/article/pii/S1672022921000772 |
| work_keys_str_mv | AT zhiliangbai singlecellanalysistechnologiesforimmunooncologyresearchfrommechanisticdelineationtobiomarkerdiscovery AT grahamsu singlecellanalysistechnologiesforimmunooncologyresearchfrommechanisticdelineationtobiomarkerdiscovery AT rongfan singlecellanalysistechnologiesforimmunooncologyresearchfrommechanisticdelineationtobiomarkerdiscovery |