Advances in the Characterization of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania
<i>Usnea barbata</i> (L.) Weber ex F.H. Wigg (<i>U. barbata</i>) is a medicinal representative of the lichens from the <i>Usnea</i> genus (<i>Parmeliaceae,</i> lichenized <i>Ascomycetes</i>), containing bioactive secondary metabolites. The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/9/4234 |
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| author | Violeta Popovici Laura Bucur Cerasela Elena Gîrd Suzana Ioana Calcan Elena Iulia Cucolea Teodor Costache Dan Rambu Mădălina Ungureanu-Iuga Mircea Oroian Silvia Mironeasa Verginica Schröder Emma Adriana Ozon Aureliana Caraiane Victoria Badea |
| author_facet | Violeta Popovici Laura Bucur Cerasela Elena Gîrd Suzana Ioana Calcan Elena Iulia Cucolea Teodor Costache Dan Rambu Mădălina Ungureanu-Iuga Mircea Oroian Silvia Mironeasa Verginica Schröder Emma Adriana Ozon Aureliana Caraiane Victoria Badea |
| author_sort | Violeta Popovici |
| collection | DOAJ |
| description | <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg (<i>U. barbata</i>) is a medicinal representative of the lichens from the <i>Usnea</i> genus (<i>Parmeliaceae,</i> lichenized <i>Ascomycetes</i>), containing bioactive secondary metabolites. The aim of this study is a comparative analysis between two separated parts of the thallus layers: medulla–cortex (<i>mc</i>UB) and central cord (<i>cc</i>UB) and the whole dried <i>U. barbata</i> thallus (<i>d</i>UB). These three samples were examined regarding color differences. The <i>U. barbata</i> thallus morphology was examined through fluorescent microscopy (FM) and scanning electron microscopy (SEM). The mineral content was measured using inductively coupled plasma mass spectrometry (ICP-MS), and Fourier transform infrared spectroscopy (FT-IR) preliminarily established the differences in the metabolite content. Finally, extracts in different solvents (ethanol and acetone) were obtained from all studied samples, and their total phenolic content (TPC) and free radical scavenging activity (antiradical activity, AA) were evaluated by spectrophotometry. The ICP-MS results showed that from 23 elements analyzed, 18 minerals were quantified in <i>mc</i>UB, 13 in <i>d</i>UB, and only 12 in <i>cc</i>UB. The <i>cc</i>UB fraction recorded the lowest mineral content, color intensity (chroma), luminosity (<i>L</i>*), and TPC value, followed in increasing order by <i>d</i>UB and <i>mc</i>UB. FT-IR spectra displayed different peaks in <i>cc</i>UB and <i>d</i>UB samples compared to <i>mc</i>UB. The <i>mc</i>UB fraction also showed the highest TPC, significantly correlated with AA. However, <i>d</i>UB had the highest antiradical activity, followed by <i>mc</i>UB and <i>cc</i>UB, with noticeable differences in the acetone extract. The final correlation between all variable data obtained indicates that 99.31% of the total variance was associated with all minerals, total phenolics, and color parameters and was also related to the antiradical activity. These obtained results complete our previous studies on autochthonous <i>U. barbata.</i> Moreover, being a source of bioactive metabolites, extracting them from the <i>mc</i>UB fraction could increase this process’s yield and selectivity. |
| first_indexed | 2024-03-10T04:23:21Z |
| format | Article |
| id | doaj.art-e7e7ff3fac6243879e36e8a6bc08daeb |
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| language | English |
| last_indexed | 2024-03-10T04:23:21Z |
| publishDate | 2022-04-01 |
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| series | Applied Sciences |
| spelling | doaj.art-e7e7ff3fac6243879e36e8a6bc08daeb2023-11-23T07:46:11ZengMDPI AGApplied Sciences2076-34172022-04-01129423410.3390/app12094234Advances in the Characterization of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from Călimani Mountains, RomaniaVioleta Popovici0Laura Bucur1Cerasela Elena Gîrd2Suzana Ioana Calcan3Elena Iulia Cucolea4Teodor Costache5Dan Rambu6Mădălina Ungureanu-Iuga7Mircea Oroian8Silvia Mironeasa9Verginica Schröder10Emma Adriana Ozon11Aureliana Caraiane12Victoria Badea13Department of Microbiology and Immunology, Faculty of Dental Medicine, Ovidius University of Constanta, 7 Ilarie Voronca Street, 900684 Constanta, RomaniaDepartment of Pharmacognosy, Faculty of Pharmacy, Ovidius University of Constanta, 6 Capitan Al. Serbanescu Street, 900001 Constanta, RomaniaDepartment of Pharmacognosy, Phytochemistry, and Phytotherapy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956 Bucharest, RomaniaResearch Center for Instrumental Analysis SCIENT, 1E Petre Ispirescu Street, 077167 Ilfov, RomaniaResearch Center for Instrumental Analysis SCIENT, 1E Petre Ispirescu Street, 077167 Ilfov, RomaniaResearch Center for Instrumental Analysis SCIENT, 1E Petre Ispirescu Street, 077167 Ilfov, RomaniaResearch Center for Instrumental Analysis SCIENT, 1E Petre Ispirescu Street, 077167 Ilfov, RomaniaFaculty of Food Engineering, Stefan cel Mare University of Suceava, 13th University Street, 720229 Suceava, RomaniaFaculty of Food Engineering, Stefan cel Mare University of Suceava, 13th University Street, 720229 Suceava, RomaniaFaculty of Food Engineering, Stefan cel Mare University of Suceava, 13th University Street, 720229 Suceava, RomaniaDepartment of Cellular and Molecular Biology, Faculty of Pharmacy, Ovidius University of Constanta, 6 Capitan Al. Serbanescu Street, 900001 Constanta, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956 Bucharest, RomaniaDepartment of Oral Rehabilitation, Faculty of Dental Medicine, Ovidius University of Constanta, 7 Ilarie Voronca Street, 900684 Constanta, RomaniaDepartment of Microbiology and Immunology, Faculty of Dental Medicine, Ovidius University of Constanta, 7 Ilarie Voronca Street, 900684 Constanta, Romania<i>Usnea barbata</i> (L.) Weber ex F.H. Wigg (<i>U. barbata</i>) is a medicinal representative of the lichens from the <i>Usnea</i> genus (<i>Parmeliaceae,</i> lichenized <i>Ascomycetes</i>), containing bioactive secondary metabolites. The aim of this study is a comparative analysis between two separated parts of the thallus layers: medulla–cortex (<i>mc</i>UB) and central cord (<i>cc</i>UB) and the whole dried <i>U. barbata</i> thallus (<i>d</i>UB). These three samples were examined regarding color differences. The <i>U. barbata</i> thallus morphology was examined through fluorescent microscopy (FM) and scanning electron microscopy (SEM). The mineral content was measured using inductively coupled plasma mass spectrometry (ICP-MS), and Fourier transform infrared spectroscopy (FT-IR) preliminarily established the differences in the metabolite content. Finally, extracts in different solvents (ethanol and acetone) were obtained from all studied samples, and their total phenolic content (TPC) and free radical scavenging activity (antiradical activity, AA) were evaluated by spectrophotometry. The ICP-MS results showed that from 23 elements analyzed, 18 minerals were quantified in <i>mc</i>UB, 13 in <i>d</i>UB, and only 12 in <i>cc</i>UB. The <i>cc</i>UB fraction recorded the lowest mineral content, color intensity (chroma), luminosity (<i>L</i>*), and TPC value, followed in increasing order by <i>d</i>UB and <i>mc</i>UB. FT-IR spectra displayed different peaks in <i>cc</i>UB and <i>d</i>UB samples compared to <i>mc</i>UB. The <i>mc</i>UB fraction also showed the highest TPC, significantly correlated with AA. However, <i>d</i>UB had the highest antiradical activity, followed by <i>mc</i>UB and <i>cc</i>UB, with noticeable differences in the acetone extract. The final correlation between all variable data obtained indicates that 99.31% of the total variance was associated with all minerals, total phenolics, and color parameters and was also related to the antiradical activity. These obtained results complete our previous studies on autochthonous <i>U. barbata.</i> Moreover, being a source of bioactive metabolites, extracting them from the <i>mc</i>UB fraction could increase this process’s yield and selectivity.https://www.mdpi.com/2076-3417/12/9/4234<i>Usnea barbata</i> (L.) Weber ex F.H. Wiggmorphologycolormineralsphenolic secondary metabolitesfree radical scavenging activity |
| spellingShingle | Violeta Popovici Laura Bucur Cerasela Elena Gîrd Suzana Ioana Calcan Elena Iulia Cucolea Teodor Costache Dan Rambu Mădălina Ungureanu-Iuga Mircea Oroian Silvia Mironeasa Verginica Schröder Emma Adriana Ozon Aureliana Caraiane Victoria Badea Advances in the Characterization of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania Applied Sciences <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg morphology color minerals phenolic secondary metabolites free radical scavenging activity |
| title | Advances in the Characterization of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania |
| title_full | Advances in the Characterization of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania |
| title_fullStr | Advances in the Characterization of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania |
| title_full_unstemmed | Advances in the Characterization of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania |
| title_short | Advances in the Characterization of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania |
| title_sort | advances in the characterization of i usnea barbata i l weber ex f h wigg from calimani mountains romania |
| topic | <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg morphology color minerals phenolic secondary metabolites free radical scavenging activity |
| url | https://www.mdpi.com/2076-3417/12/9/4234 |
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