In vitro and in vivo antiplasmodial assays of selected Nigerian commercial herbal formulations
Introduction: Malaria remains a life-threatening disease, mainly in tropical and sub-tropical countries of the world. The problem caused by the disease is further compounded by the emergence and spread of multidrug resistant Plasmodium falciparum. Coupled with the poor distribution of modern health...
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| Format: | Article |
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Shahrekord University of Medical Sciences
2020-10-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | http://herbmedpharmacol.com/PDF/jhp-9-374.pdf |
| _version_ | 1818870511734620160 |
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| author | Chinedu Joseph Ikem Regina Appiah-opong Angus Nnamdi Oli Malachy Chigozie Ugwu Patrick Amoateng Kojo Agyemang David Chinemerem Nwobodo Charles Okechukwu Esimone |
| author_facet | Chinedu Joseph Ikem Regina Appiah-opong Angus Nnamdi Oli Malachy Chigozie Ugwu Patrick Amoateng Kojo Agyemang David Chinemerem Nwobodo Charles Okechukwu Esimone |
| author_sort | Chinedu Joseph Ikem |
| collection | DOAJ |
| description | Introduction: Malaria remains a life-threatening disease, mainly in tropical and sub-tropical countries of the world. The problem caused by the disease is further compounded by the emergence and spread of multidrug resistant Plasmodium falciparum. Coupled with the poor distribution of modern health facilities, there is resurgence in the use of herbal remedies to treat malaria. In this study, we evaluated the antiplasmodial activities of six commercially available herbal formulations using in vivo and in vitro methods to assess their claimed antimalarial properties. Methods: The antiplasmodial activities of the six herbal formulations were assessed using Chloroquine sensitive P. falciparum parasite strain 3D7 using the SYBR Green in vitro method and the in vivo curative test (established infection) in Plasmodium berghei infected Mus musculus. Results: The six herbal formulations had values of IC50 > 100 μg/mL on 3D7 strain of P. falciparum compared to controls which had IC50 values of 6.92nM (Chloroquine) and 0.75nM (Artesunate). In the curative evaluation (in vivo) the herbal formulations significantly reduced parasitaemia on day 4 (26.3%-77.3 %) and day 7 (45.54%-94.81%) post-treatments (P < 0.05) when compared to the untreated group, which recorded high mortality rate. Conclusion: Findings made in this study lend support to the claim that these herbal formulations have antiplasmodial activities. Percentage inhibitions of parasitaemia of the formulations were all above 50% except M&T capsule which had lower percentage inhibition of parasitaemia. |
| first_indexed | 2024-12-19T12:08:11Z |
| format | Article |
| id | doaj.art-e7ee3125622245268b03f49aac94ceca |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-12-19T12:08:11Z |
| publishDate | 2020-10-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-e7ee3125622245268b03f49aac94ceca2022-12-21T20:22:18ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042020-10-019437438110.34172/jhp.2020.47jhp-30557In vitro and in vivo antiplasmodial assays of selected Nigerian commercial herbal formulationsChinedu Joseph Ikem0Regina Appiah-opong1Angus Nnamdi Oli2Malachy Chigozie Ugwu3Patrick Amoateng4Kojo Agyemang5David Chinemerem Nwobodo6Charles Okechukwu Esimone7Department of Pharmaceutical Microbiology & Biotechnology, Faculty of Pharmaceutical Sciences, Madonna University, Elele, River State, NigeriaDepartment of Clinical Pathology, Noguchi Memorial institute for Medical Research, College of Health Sciences, University of Ghana, GhanaDepartment of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaDepartment of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaDepartment of Pharmacology & Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, GhanaDepartment of Pharmacology & Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, GhanaDepartment of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaDepartment of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaIntroduction: Malaria remains a life-threatening disease, mainly in tropical and sub-tropical countries of the world. The problem caused by the disease is further compounded by the emergence and spread of multidrug resistant Plasmodium falciparum. Coupled with the poor distribution of modern health facilities, there is resurgence in the use of herbal remedies to treat malaria. In this study, we evaluated the antiplasmodial activities of six commercially available herbal formulations using in vivo and in vitro methods to assess their claimed antimalarial properties. Methods: The antiplasmodial activities of the six herbal formulations were assessed using Chloroquine sensitive P. falciparum parasite strain 3D7 using the SYBR Green in vitro method and the in vivo curative test (established infection) in Plasmodium berghei infected Mus musculus. Results: The six herbal formulations had values of IC50 > 100 μg/mL on 3D7 strain of P. falciparum compared to controls which had IC50 values of 6.92nM (Chloroquine) and 0.75nM (Artesunate). In the curative evaluation (in vivo) the herbal formulations significantly reduced parasitaemia on day 4 (26.3%-77.3 %) and day 7 (45.54%-94.81%) post-treatments (P < 0.05) when compared to the untreated group, which recorded high mortality rate. Conclusion: Findings made in this study lend support to the claim that these herbal formulations have antiplasmodial activities. Percentage inhibitions of parasitaemia of the formulations were all above 50% except M&T capsule which had lower percentage inhibition of parasitaemia.http://herbmedpharmacol.com/PDF/jhp-9-374.pdfantiplasmodial activityherbal formulationsmalariasybr greenplasmodium falciparumplasmodium berghei |
| spellingShingle | Chinedu Joseph Ikem Regina Appiah-opong Angus Nnamdi Oli Malachy Chigozie Ugwu Patrick Amoateng Kojo Agyemang David Chinemerem Nwobodo Charles Okechukwu Esimone In vitro and in vivo antiplasmodial assays of selected Nigerian commercial herbal formulations Journal of HerbMed Pharmacology antiplasmodial activity herbal formulations malaria sybr green plasmodium falciparum plasmodium berghei |
| title | In vitro and in vivo antiplasmodial assays of selected Nigerian commercial herbal formulations |
| title_full | In vitro and in vivo antiplasmodial assays of selected Nigerian commercial herbal formulations |
| title_fullStr | In vitro and in vivo antiplasmodial assays of selected Nigerian commercial herbal formulations |
| title_full_unstemmed | In vitro and in vivo antiplasmodial assays of selected Nigerian commercial herbal formulations |
| title_short | In vitro and in vivo antiplasmodial assays of selected Nigerian commercial herbal formulations |
| title_sort | in vitro and in vivo antiplasmodial assays of selected nigerian commercial herbal formulations |
| topic | antiplasmodial activity herbal formulations malaria sybr green plasmodium falciparum plasmodium berghei |
| url | http://herbmedpharmacol.com/PDF/jhp-9-374.pdf |
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