Seroprevalence and molecular investigation of toxocariasis in working children and control group in Tehran, Iran
Background: Toxocariasis, a zoonosis helminthic disease is caused in humans by Toxocara canis and Toxocara cati with worldwide distribution. In this study the seroprevalence and molecular toxocariasis were investigated in the working children and the control group in Tehran, Iran. Methods: In total,...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Clinical Epidemiology and Global Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221339842400068X |
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| author | Farah Bokharaei-Salim Khadijeh Khanaliha Tayyeb Ghadimi Roghayeh Babaei Mohsen Sadeghi Borna Salemi |
| author_facet | Farah Bokharaei-Salim Khadijeh Khanaliha Tayyeb Ghadimi Roghayeh Babaei Mohsen Sadeghi Borna Salemi |
| author_sort | Farah Bokharaei-Salim |
| collection | DOAJ |
| description | Background: Toxocariasis, a zoonosis helminthic disease is caused in humans by Toxocara canis and Toxocara cati with worldwide distribution. In this study the seroprevalence and molecular toxocariasis were investigated in the working children and the control group in Tehran, Iran. Methods: In total, 468 children between the ages of 7–14 years old, including 280 blood samples from working children and 188 samples from control group children were collected from Tehran. ELISA test was performed to detect IgG antibody against Toxocara canis. Molecular tests using ITS2 primer were performed to determine the status of toxocariasis infection in children. Results: The seroprevalence of toxocariasis was 24 (8.6%) and 10 (5.3%) in the working children and control group respectively. Seroprevalence of toxocariasis in girls 18(13.5%) was higher than boys 6(4.1%) in working children and differences was statistically significant. A significant relationship was found between contact with soil or contact with cats and dogs and the seroprevalence of toxocariasis in working children. No amplification of the target DNA was detected in plasma or whole blood samples in working children and the control group with Toxocara canis and cati primers. Conclusion: The seroprevalence of IgG antibody against Toxocara canis was higher in working children than in the control group in Tehran. The result of the present study indicated a significant difference between working children and the control group in terms of Toxocara canis antibody titer. More contact with soil in these children and contact with dogs and cats may be factors in acquiring toxocariasis in the children. |
| first_indexed | 2024-04-24T23:24:21Z |
| format | Article |
| id | doaj.art-e7eeb695416d4435bc38b015607c5aad |
| institution | Directory Open Access Journal |
| issn | 2213-3984 |
| language | English |
| last_indexed | 2025-03-21T20:56:20Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Clinical Epidemiology and Global Health |
| spelling | doaj.art-e7eeb695416d4435bc38b015607c5aad2024-05-31T05:40:18ZengElsevierClinical Epidemiology and Global Health2213-39842024-05-0127101572Seroprevalence and molecular investigation of toxocariasis in working children and control group in Tehran, IranFarah Bokharaei-Salim0Khadijeh Khanaliha1Tayyeb Ghadimi2Roghayeh Babaei3Mohsen Sadeghi4Borna Salemi5Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, IranResearch Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran; Corresponding author. Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran.Burn Research Center, Iran University of Medical Sciences, Tehran, IranDeputy of Health, Iran University of Medical Sciences, Tehran, IranDeputy of Health, Iran University of Medical Sciences, Tehran, IranStudent Research Committee, Fasa University of Medical Science, Fasa, IranBackground: Toxocariasis, a zoonosis helminthic disease is caused in humans by Toxocara canis and Toxocara cati with worldwide distribution. In this study the seroprevalence and molecular toxocariasis were investigated in the working children and the control group in Tehran, Iran. Methods: In total, 468 children between the ages of 7–14 years old, including 280 blood samples from working children and 188 samples from control group children were collected from Tehran. ELISA test was performed to detect IgG antibody against Toxocara canis. Molecular tests using ITS2 primer were performed to determine the status of toxocariasis infection in children. Results: The seroprevalence of toxocariasis was 24 (8.6%) and 10 (5.3%) in the working children and control group respectively. Seroprevalence of toxocariasis in girls 18(13.5%) was higher than boys 6(4.1%) in working children and differences was statistically significant. A significant relationship was found between contact with soil or contact with cats and dogs and the seroprevalence of toxocariasis in working children. No amplification of the target DNA was detected in plasma or whole blood samples in working children and the control group with Toxocara canis and cati primers. Conclusion: The seroprevalence of IgG antibody against Toxocara canis was higher in working children than in the control group in Tehran. The result of the present study indicated a significant difference between working children and the control group in terms of Toxocara canis antibody titer. More contact with soil in these children and contact with dogs and cats may be factors in acquiring toxocariasis in the children.http://www.sciencedirect.com/science/article/pii/S221339842400068XSeroprevalenceElisaToxocariasisWorking childrenToxocara canis |
| spellingShingle | Farah Bokharaei-Salim Khadijeh Khanaliha Tayyeb Ghadimi Roghayeh Babaei Mohsen Sadeghi Borna Salemi Seroprevalence and molecular investigation of toxocariasis in working children and control group in Tehran, Iran Clinical Epidemiology and Global Health Seroprevalence Elisa Toxocariasis Working children Toxocara canis |
| title | Seroprevalence and molecular investigation of toxocariasis in working children and control group in Tehran, Iran |
| title_full | Seroprevalence and molecular investigation of toxocariasis in working children and control group in Tehran, Iran |
| title_fullStr | Seroprevalence and molecular investigation of toxocariasis in working children and control group in Tehran, Iran |
| title_full_unstemmed | Seroprevalence and molecular investigation of toxocariasis in working children and control group in Tehran, Iran |
| title_short | Seroprevalence and molecular investigation of toxocariasis in working children and control group in Tehran, Iran |
| title_sort | seroprevalence and molecular investigation of toxocariasis in working children and control group in tehran iran |
| topic | Seroprevalence Elisa Toxocariasis Working children Toxocara canis |
| url | http://www.sciencedirect.com/science/article/pii/S221339842400068X |
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