Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia
Abstract Introduction During liver surgery and transplantation, periods of partial or total vascular occlusion are inevitable and result in ischemia-reperfusion injury (IRI). Nanomedicine uses the latest technology, which has emerged with interdisciplinary effects, such as biomedical sciences, physi...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | BMC Anesthesiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12871-023-01999-0 |
| _version_ | 1811171595382685696 |
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| author | Huseyin Gobut Aysegul Kucuk Necmiye Şengel Mustafa Arslan Cagrı Ozdemir Tulay Mortas Esat Kasapbası Omer Kurtipek Mustafa Kavutcu |
| author_facet | Huseyin Gobut Aysegul Kucuk Necmiye Şengel Mustafa Arslan Cagrı Ozdemir Tulay Mortas Esat Kasapbası Omer Kurtipek Mustafa Kavutcu |
| author_sort | Huseyin Gobut |
| collection | DOAJ |
| description | Abstract Introduction During liver surgery and transplantation, periods of partial or total vascular occlusion are inevitable and result in ischemia-reperfusion injury (IRI). Nanomedicine uses the latest technology, which has emerged with interdisciplinary effects, such as biomedical sciences, physics, and engineering, to protect and improve human health. Interdisciplinary research has brought along the introduction of antioxidant nanoparticles as potential therapeutics. The goal of this study was to investigate the effects of cerium oxide (CeO2) administration and desflurane anesthesia on liver tissue in liver IR injury. Material and methods Thirty rats were randomly divided into five groups: control (C), ischemia-reperfusion (IR), IR-desflurane (IRD), cerium oxide-ischemia reperfusion (CeO2-IR), and cerium oxide-ischemia reperfusion-desflurane (CeO2-IRD). In the IR, IRD, and CeO2-IRD groups, hepatic ischemia was induced after the porta hepatis was clamped for 120 min, followed by 120 min of reperfusion. Intraperitoneal 0.5 mg/kg CeO2 was administered to the CeO2 groups 30 min before ischemia. Desflurane (6%) was administered to the IRD and CeO2-IRD groups during IR. All groups were sacrificed under anesthesia. Liver tissue samples were examined under a light microscope by staining with hematoxylin-eosin (H&E). Malondialdehyde (MDA) levels, catalase (CAT), glutathione-s-transferase (GST), and arylesterase (ARE) enzyme activities were measured in the tissue samples. Results The IR group had considerably more hydropic degeneration, sinusoidal dilatation, and parenchymal mononuclear cell infiltration than the IRD, CeO2-IR, and CeO2-IRD groups. Catalase and GST enzyme activity were significantly higher in the CeO2-IR group than in the IR group. The MDA levels were found to be significantly lower in the IRD, CeO2-IR, and CeO2-IRD groups than in the IR group. Conclusion Intraperitoneal CeO2 with desflurane reduced oxidative stress and corrected liver damage. |
| first_indexed | 2024-04-10T17:16:42Z |
| format | Article |
| id | doaj.art-e7f0aacf0f5c4d41a7370b7bb7e5ad52 |
| institution | Directory Open Access Journal |
| issn | 1471-2253 |
| language | English |
| last_indexed | 2024-04-10T17:16:42Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Anesthesiology |
| spelling | doaj.art-e7f0aacf0f5c4d41a7370b7bb7e5ad522023-02-05T12:21:59ZengBMCBMC Anesthesiology1471-22532023-02-0123111110.1186/s12871-023-01999-0Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesiaHuseyin Gobut0Aysegul Kucuk1Necmiye Şengel2Mustafa Arslan3Cagrı Ozdemir4Tulay Mortas5Esat Kasapbası6Omer Kurtipek7Mustafa Kavutcu8Department of General Surgery, Gazi University Faculty of MedicineDepartment of Physiology, Kutahya Health Sciences University Faculty of MedicineDepartment of Oral and Maxillofacial Surgery (as a specialist in Anesthesiology and Reanimation), Gazi University Faculty of DentistryDepartment of Anesthesiology and Reanimation, Gazi University Faculty of MedicineDepartment of Anesthesiology and Reanimation, Gazi University Faculty of MedicineDepartment of Histology and Embryology, Kırıkkale University Faculty of MedicineDepartment of Anesthesiology and Reanimation, Gazi University Faculty of MedicineDepartment of Anesthesiology and Reanimation, Gazi University Faculty of MedicineDepartment of Medical Biochemistry, Gazi University Faculty of MedicineAbstract Introduction During liver surgery and transplantation, periods of partial or total vascular occlusion are inevitable and result in ischemia-reperfusion injury (IRI). Nanomedicine uses the latest technology, which has emerged with interdisciplinary effects, such as biomedical sciences, physics, and engineering, to protect and improve human health. Interdisciplinary research has brought along the introduction of antioxidant nanoparticles as potential therapeutics. The goal of this study was to investigate the effects of cerium oxide (CeO2) administration and desflurane anesthesia on liver tissue in liver IR injury. Material and methods Thirty rats were randomly divided into five groups: control (C), ischemia-reperfusion (IR), IR-desflurane (IRD), cerium oxide-ischemia reperfusion (CeO2-IR), and cerium oxide-ischemia reperfusion-desflurane (CeO2-IRD). In the IR, IRD, and CeO2-IRD groups, hepatic ischemia was induced after the porta hepatis was clamped for 120 min, followed by 120 min of reperfusion. Intraperitoneal 0.5 mg/kg CeO2 was administered to the CeO2 groups 30 min before ischemia. Desflurane (6%) was administered to the IRD and CeO2-IRD groups during IR. All groups were sacrificed under anesthesia. Liver tissue samples were examined under a light microscope by staining with hematoxylin-eosin (H&E). Malondialdehyde (MDA) levels, catalase (CAT), glutathione-s-transferase (GST), and arylesterase (ARE) enzyme activities were measured in the tissue samples. Results The IR group had considerably more hydropic degeneration, sinusoidal dilatation, and parenchymal mononuclear cell infiltration than the IRD, CeO2-IR, and CeO2-IRD groups. Catalase and GST enzyme activity were significantly higher in the CeO2-IR group than in the IR group. The MDA levels were found to be significantly lower in the IRD, CeO2-IR, and CeO2-IRD groups than in the IR group. Conclusion Intraperitoneal CeO2 with desflurane reduced oxidative stress and corrected liver damage.https://doi.org/10.1186/s12871-023-01999-0Cerium oxideDesfluraneIschemia-reperfusionLiverBiochemical analysisHistopathological analysis |
| spellingShingle | Huseyin Gobut Aysegul Kucuk Necmiye Şengel Mustafa Arslan Cagrı Ozdemir Tulay Mortas Esat Kasapbası Omer Kurtipek Mustafa Kavutcu Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia BMC Anesthesiology Cerium oxide Desflurane Ischemia-reperfusion Liver Biochemical analysis Histopathological analysis |
| title | Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia |
| title_full | Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia |
| title_fullStr | Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia |
| title_full_unstemmed | Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia |
| title_short | Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia |
| title_sort | effects of cerium oxide ceo2 on liver tissue in liver ischemia reperfusion injury in rats undergoing desflurane anesthesia |
| topic | Cerium oxide Desflurane Ischemia-reperfusion Liver Biochemical analysis Histopathological analysis |
| url | https://doi.org/10.1186/s12871-023-01999-0 |
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