Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus)
The sea cucumber (Apostichopus japonicus) is the main economic species in China and has a significant role in aquaculture. Gut microbiome composition is closely related to external environments. In this study, we identified the effects of seasonal changes on the composition and main metabolites of s...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.953388/full |
| _version_ | 1811331307889754112 |
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| author | Beini Deng Beini Deng Beini Deng Xiaoshang Ru Xiaoshang Ru Xiaoshang Ru Xiaoshang Ru Xiaoshang Ru Ting Wang Ting Wang Chenxi Zhang Chenxi Zhang Wanhui Sun Songchong Lu Libin Zhang Libin Zhang Libin Zhang Libin Zhang Libin Zhang |
| author_facet | Beini Deng Beini Deng Beini Deng Xiaoshang Ru Xiaoshang Ru Xiaoshang Ru Xiaoshang Ru Xiaoshang Ru Ting Wang Ting Wang Chenxi Zhang Chenxi Zhang Wanhui Sun Songchong Lu Libin Zhang Libin Zhang Libin Zhang Libin Zhang Libin Zhang |
| author_sort | Beini Deng |
| collection | DOAJ |
| description | The sea cucumber (Apostichopus japonicus) is the main economic species in China and has a significant role in aquaculture. Gut microbiome composition is closely related to external environments. In this study, we identified the effects of seasonal changes on the composition and main metabolites of symbiotic microorganisms in the intestine of A. japonicus. We used 16S rRNA sequencing to identify the composition of symbiotic microorganisms in different seasons. Intestinal metabolites were determined using liquid chromatography with tandem mass spectrometry, which linked symbiotic microorganisms to intestinal metabolites. Analyzing changes in intestinal microbial composition across different seasons. The results showed that seasonal changes of intestinal microorganisms were significant, A. japonicus were infected by Vibrio easily in summer, Stigmasterol and sitosterol could affect the growth of body wall of A. japonicus. It is vital importance for A. japonicus that the results benefit for the growth, immunity and aquaculture. |
| first_indexed | 2024-04-13T16:18:37Z |
| format | Article |
| id | doaj.art-e7f1b29d44a84417a5a1cb1ae3dd4dec |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-13T16:18:37Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-e7f1b29d44a84417a5a1cb1ae3dd4dec2022-12-22T02:40:00ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-11-01910.3389/fmars.2022.953388953388Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus)Beini Deng0Beini Deng1Beini Deng2Xiaoshang Ru3Xiaoshang Ru4Xiaoshang Ru5Xiaoshang Ru6Xiaoshang Ru7Ting Wang8Ting Wang9Chenxi Zhang10Chenxi Zhang11Wanhui Sun12Songchong Lu13Libin Zhang14Libin Zhang15Libin Zhang16Libin Zhang17Libin Zhang18College of Life Sciences, Qingdao Agricultural University, Qingdao, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCenter for Ocean Mega-Sciences, Chinese Academy of Sciences, Qingdao, ChinaShandong Province Key Laboratory of Experimental Marine Biology, Qingdao, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaizhou Municipal Bureau of Marine Development And Fisheries, Yantai, ChinaCollege of Life Sciences, Qingdao Agricultural University, Qingdao, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCenter for Ocean Mega-Sciences, Chinese Academy of Sciences, Qingdao, ChinaShandong Province Key Laboratory of Experimental Marine Biology, Qingdao, ChinaThe sea cucumber (Apostichopus japonicus) is the main economic species in China and has a significant role in aquaculture. Gut microbiome composition is closely related to external environments. In this study, we identified the effects of seasonal changes on the composition and main metabolites of symbiotic microorganisms in the intestine of A. japonicus. We used 16S rRNA sequencing to identify the composition of symbiotic microorganisms in different seasons. Intestinal metabolites were determined using liquid chromatography with tandem mass spectrometry, which linked symbiotic microorganisms to intestinal metabolites. Analyzing changes in intestinal microbial composition across different seasons. The results showed that seasonal changes of intestinal microorganisms were significant, A. japonicus were infected by Vibrio easily in summer, Stigmasterol and sitosterol could affect the growth of body wall of A. japonicus. It is vital importance for A. japonicus that the results benefit for the growth, immunity and aquaculture.https://www.frontiersin.org/articles/10.3389/fmars.2022.953388/full16S rRNAgut microbiomemetabolomicssea cucumber (Apostichopus japonicus)seasonal variations |
| spellingShingle | Beini Deng Beini Deng Beini Deng Xiaoshang Ru Xiaoshang Ru Xiaoshang Ru Xiaoshang Ru Xiaoshang Ru Ting Wang Ting Wang Chenxi Zhang Chenxi Zhang Wanhui Sun Songchong Lu Libin Zhang Libin Zhang Libin Zhang Libin Zhang Libin Zhang Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus) Frontiers in Marine Science 16S rRNA gut microbiome metabolomics sea cucumber (Apostichopus japonicus) seasonal variations |
| title | Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus) |
| title_full | Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus) |
| title_fullStr | Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus) |
| title_full_unstemmed | Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus) |
| title_short | Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus) |
| title_sort | seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber apostichopus japonicus |
| topic | 16S rRNA gut microbiome metabolomics sea cucumber (Apostichopus japonicus) seasonal variations |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.953388/full |
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