Detection of SARS-CoV-2 Neutralizing Antibodies in Vaccinated Pregnant Women and Neonates by Using a Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader
The focus of this study was to investigate the detection of neutralizing antibodies (Nabs) in maternal serum and cord blood as the targeted samples by employing a lateral flow immunoassay combined with a spectrum reader (LFI-SR) and the correlation of Nab protection against different types of SARS-C...
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MDPI AG
2022-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/891 |
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| author | Wei-Chun Chen Yen-Pin Lin Chao-Min Cheng Ching-Fen Shen Chang-Wei Li Yu-Kuo Wang Ting-Ying Shih Chitsung Hong Ting-Chang Chang Ching-Ju Shen |
| author_facet | Wei-Chun Chen Yen-Pin Lin Chao-Min Cheng Ching-Fen Shen Chang-Wei Li Yu-Kuo Wang Ting-Ying Shih Chitsung Hong Ting-Chang Chang Ching-Ju Shen |
| author_sort | Wei-Chun Chen |
| collection | DOAJ |
| description | The focus of this study was to investigate the detection of neutralizing antibodies (Nabs) in maternal serum and cord blood as the targeted samples by employing a lateral flow immunoassay combined with a spectrum reader (LFI-SR) and the correlation of Nab protection against different types of SARS-CoV-2. We enrolled 20 pregnant women who were vaccinated with the Moderna (mRNA-1273) vaccine during pregnancy and collected 40 samples during delivery. We used an LFI-SR for the level of spike protein receptor binding domain antibody (SRBD IgG) as Nabs and examined the correlation of the SRBD IgG concentration and Nab inhibition rates (NabIR) via enzyme-linked immunosorbent assays (ELISA). The LFI-SR had high confidence for the SRBD IgG level (<i>p</i> < 0.0001). Better NabIR were found in wild-type SARS-CoV-2 (WT) compared to Delta-type (DT) and Omicron-type (OT). Women with two-dose vaccinations demonstrated greater NabIR than those with a single dose. The cut-off value of the SRBD IgG level by the LFI-SR for NabIR to DT (≥30%; ≥70%) was 60.15 and 150.21 ng/mL for mothers (both <i>p</i> = 0.005), and 156.31 (<i>p</i> = 0.011) and 230.20 ng/mL (<i>p</i> = 0.006) for babies, respectively. An additional vaccine booster may be considered for those mothers with SRBD IgG levels < 60.15 ng/mL, and close protection should be given for those neonates with SRBD IgG levels < 150.21 ng/mL, since there is no available vaccine for them. |
| first_indexed | 2024-03-09T20:35:28Z |
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| id | doaj.art-e7f247027e77446587f8a23218fe40ae |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:35:28Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-e7f247027e77446587f8a23218fe40ae2023-11-23T23:12:20ZengMDPI AGBiosensors2079-63742022-10-01121089110.3390/bios12100891Detection of SARS-CoV-2 Neutralizing Antibodies in Vaccinated Pregnant Women and Neonates by Using a Lateral Flow Immunoassay Coupled with a Spectrum-Based ReaderWei-Chun Chen0Yen-Pin Lin1Chao-Min Cheng2Ching-Fen Shen3Chang-Wei Li4Yu-Kuo Wang5Ting-Ying Shih6Chitsung Hong7Ting-Chang Chang8Ching-Ju Shen9Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, TaiwanInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, TaiwanInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, TaiwanDepartment of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanAllBio Life Inc., Taichung 408, TaiwanAllBio Life Inc., Taichung 408, TaiwanAllBio Life Inc., Taichung 408, TaiwanSpectrochip Inc., Hsinchu 302, TaiwanDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital at Linkou, College of Medicine, Chang Gung University, Taoyuan 333, TaiwanDepartment of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanThe focus of this study was to investigate the detection of neutralizing antibodies (Nabs) in maternal serum and cord blood as the targeted samples by employing a lateral flow immunoassay combined with a spectrum reader (LFI-SR) and the correlation of Nab protection against different types of SARS-CoV-2. We enrolled 20 pregnant women who were vaccinated with the Moderna (mRNA-1273) vaccine during pregnancy and collected 40 samples during delivery. We used an LFI-SR for the level of spike protein receptor binding domain antibody (SRBD IgG) as Nabs and examined the correlation of the SRBD IgG concentration and Nab inhibition rates (NabIR) via enzyme-linked immunosorbent assays (ELISA). The LFI-SR had high confidence for the SRBD IgG level (<i>p</i> < 0.0001). Better NabIR were found in wild-type SARS-CoV-2 (WT) compared to Delta-type (DT) and Omicron-type (OT). Women with two-dose vaccinations demonstrated greater NabIR than those with a single dose. The cut-off value of the SRBD IgG level by the LFI-SR for NabIR to DT (≥30%; ≥70%) was 60.15 and 150.21 ng/mL for mothers (both <i>p</i> = 0.005), and 156.31 (<i>p</i> = 0.011) and 230.20 ng/mL (<i>p</i> = 0.006) for babies, respectively. An additional vaccine booster may be considered for those mothers with SRBD IgG levels < 60.15 ng/mL, and close protection should be given for those neonates with SRBD IgG levels < 150.21 ng/mL, since there is no available vaccine for them.https://www.mdpi.com/2079-6374/12/10/891COVID-19 vaccinewild-type SARS-CoV-2Delta-type SARS-CoV-2Omicron-type SARS-CoV-2S1RBD IgGneutralizing antibodies |
| spellingShingle | Wei-Chun Chen Yen-Pin Lin Chao-Min Cheng Ching-Fen Shen Chang-Wei Li Yu-Kuo Wang Ting-Ying Shih Chitsung Hong Ting-Chang Chang Ching-Ju Shen Detection of SARS-CoV-2 Neutralizing Antibodies in Vaccinated Pregnant Women and Neonates by Using a Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader Biosensors COVID-19 vaccine wild-type SARS-CoV-2 Delta-type SARS-CoV-2 Omicron-type SARS-CoV-2 S1RBD IgG neutralizing antibodies |
| title | Detection of SARS-CoV-2 Neutralizing Antibodies in Vaccinated Pregnant Women and Neonates by Using a Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader |
| title_full | Detection of SARS-CoV-2 Neutralizing Antibodies in Vaccinated Pregnant Women and Neonates by Using a Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader |
| title_fullStr | Detection of SARS-CoV-2 Neutralizing Antibodies in Vaccinated Pregnant Women and Neonates by Using a Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader |
| title_full_unstemmed | Detection of SARS-CoV-2 Neutralizing Antibodies in Vaccinated Pregnant Women and Neonates by Using a Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader |
| title_short | Detection of SARS-CoV-2 Neutralizing Antibodies in Vaccinated Pregnant Women and Neonates by Using a Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader |
| title_sort | detection of sars cov 2 neutralizing antibodies in vaccinated pregnant women and neonates by using a lateral flow immunoassay coupled with a spectrum based reader |
| topic | COVID-19 vaccine wild-type SARS-CoV-2 Delta-type SARS-CoV-2 Omicron-type SARS-CoV-2 S1RBD IgG neutralizing antibodies |
| url | https://www.mdpi.com/2079-6374/12/10/891 |
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