General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes
The tubulin cytoskeleton plays an important role in establishing legume–rhizobial symbiosis at all stages of its development. Previously, tubulin cytoskeleton organization was studied in detail in the indeterminate nodules of two legume species, <i>Pisum sativum</i> and <i>Medicago...
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MDPI AG
2021-04-01
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| author | Anna B. Kitaeva Artemii P. Gorshkov Evgenii A. Kirichek Pyotr G. Kusakin Anna V. Tsyganova Viktor E. Tsyganov |
| author_facet | Anna B. Kitaeva Artemii P. Gorshkov Evgenii A. Kirichek Pyotr G. Kusakin Anna V. Tsyganova Viktor E. Tsyganov |
| author_sort | Anna B. Kitaeva |
| collection | DOAJ |
| description | The tubulin cytoskeleton plays an important role in establishing legume–rhizobial symbiosis at all stages of its development. Previously, tubulin cytoskeleton organization was studied in detail in the indeterminate nodules of two legume species, <i>Pisum sativum</i> and <i>Medicago truncatula</i>. General as well as species-specific patterns were revealed. To further the understanding of the formation of general and species-specific microtubule patterns in indeterminate nodules, the tubulin cytoskeleton organization was studied in three legume species (<i>Vicia sativa</i>, <i>Galega orientalis</i>, and <i>Cicer arietinum</i>). It is shown that these species differ in the shape and size of rhizobial cells (bacteroids). Immunolocalization of microtubules revealed the universality of cortical and endoplasmic microtubule organization in the meristematic cells, infected cells of the infection zone, and uninfected cells in nodules of the three species. However, there are differences in the endoplasmic microtubule organization in nitrogen-fixing cells among the species, as confirmed by quantitative analysis. It appears that the differences are linked to bacteroid morphology (both shape and size). |
| first_indexed | 2024-03-10T11:59:24Z |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T11:59:24Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-e7f4278c67434544b06d1474cef1507b2023-11-21T17:05:38ZengMDPI AGCells2073-44092021-04-01105101210.3390/cells10051012General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three LegumesAnna B. Kitaeva0Artemii P. Gorshkov1Evgenii A. Kirichek2Pyotr G. Kusakin3Anna V. Tsyganova4Viktor E. Tsyganov5Laboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chaussee 3, Pushkin 8, 196608 Saint-Petersburg, RussiaLaboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chaussee 3, Pushkin 8, 196608 Saint-Petersburg, RussiaLaboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chaussee 3, Pushkin 8, 196608 Saint-Petersburg, RussiaLaboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chaussee 3, Pushkin 8, 196608 Saint-Petersburg, RussiaLaboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chaussee 3, Pushkin 8, 196608 Saint-Petersburg, RussiaLaboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chaussee 3, Pushkin 8, 196608 Saint-Petersburg, RussiaThe tubulin cytoskeleton plays an important role in establishing legume–rhizobial symbiosis at all stages of its development. Previously, tubulin cytoskeleton organization was studied in detail in the indeterminate nodules of two legume species, <i>Pisum sativum</i> and <i>Medicago truncatula</i>. General as well as species-specific patterns were revealed. To further the understanding of the formation of general and species-specific microtubule patterns in indeterminate nodules, the tubulin cytoskeleton organization was studied in three legume species (<i>Vicia sativa</i>, <i>Galega orientalis</i>, and <i>Cicer arietinum</i>). It is shown that these species differ in the shape and size of rhizobial cells (bacteroids). Immunolocalization of microtubules revealed the universality of cortical and endoplasmic microtubule organization in the meristematic cells, infected cells of the infection zone, and uninfected cells in nodules of the three species. However, there are differences in the endoplasmic microtubule organization in nitrogen-fixing cells among the species, as confirmed by quantitative analysis. It appears that the differences are linked to bacteroid morphology (both shape and size).https://www.mdpi.com/2073-4409/10/5/1012legume–rhizobial symbiosismicrotubulessymbiosomebacteroidindeterminate nodulesimmunolocalization |
| spellingShingle | Anna B. Kitaeva Artemii P. Gorshkov Evgenii A. Kirichek Pyotr G. Kusakin Anna V. Tsyganova Viktor E. Tsyganov General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes Cells legume–rhizobial symbiosis microtubules symbiosome bacteroid indeterminate nodules immunolocalization |
| title | General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes |
| title_full | General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes |
| title_fullStr | General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes |
| title_full_unstemmed | General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes |
| title_short | General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes |
| title_sort | general patterns and species specific differences in the organization of the tubulin cytoskeleton in indeterminate nodules of three legumes |
| topic | legume–rhizobial symbiosis microtubules symbiosome bacteroid indeterminate nodules immunolocalization |
| url | https://www.mdpi.com/2073-4409/10/5/1012 |
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