Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss
Abstract Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-99066-6 |
| _version_ | 1818691923063341056 |
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| author | Abdonaser Poursalavati Sajad Rashidi-Monfared Amin Ebrahimi |
| author_facet | Abdonaser Poursalavati Sajad Rashidi-Monfared Amin Ebrahimi |
| author_sort | Abdonaser Poursalavati |
| collection | DOAJ |
| description | Abstract Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valuable secondary metabolites with antioxidant and anticancer properties, was sequenced. Then functional annotation and gene ontology analysis for 165,597 assembled transcripts were performed, most were associated with the metabolic pathways. This might be because there are various active biochemical pathways in this plant. Furthermore, after comprehensive transcript annotation, the putative genes involved in the main metabolic pathways of D. kotschyi were identified. Then, the biosynthetic pathway of its valuable methoxylated flavones was proposed. Finally, the accumulations of important methoxylated-flavone metabolites in three different tissues were quantified by HPLC. The relative expression of the genes involved in the proposed pathway was investigated by qRT-PCR, which indicated high expression levels in the bud tissue. The present results may lead to the design strategies to preserve the genetic diversity of endangered D. kotschyi plants and apply the new methods for engineering its valuable methoxylated-flavones pathway. |
| first_indexed | 2024-12-17T12:49:36Z |
| format | Article |
| id | doaj.art-e7f4ce12677344d58896e53f0755e0a3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T12:49:36Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e7f4ce12677344d58896e53f0755e0a32022-12-21T21:47:39ZengNature PortfolioScientific Reports2045-23222021-10-0111111310.1038/s41598-021-99066-6Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi BoissAbdonaser Poursalavati0Sajad Rashidi-Monfared1Amin Ebrahimi2Agricultural Biotechnology Department, Faculty of Agriculture, Tarbiat Modares UniversityAgricultural Biotechnology Department, Faculty of Agriculture, Tarbiat Modares UniversityAgronomy and Plant Breeding Department, Faculty of Agriculture, Shahrood University of TechnologyAbstract Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valuable secondary metabolites with antioxidant and anticancer properties, was sequenced. Then functional annotation and gene ontology analysis for 165,597 assembled transcripts were performed, most were associated with the metabolic pathways. This might be because there are various active biochemical pathways in this plant. Furthermore, after comprehensive transcript annotation, the putative genes involved in the main metabolic pathways of D. kotschyi were identified. Then, the biosynthetic pathway of its valuable methoxylated flavones was proposed. Finally, the accumulations of important methoxylated-flavone metabolites in three different tissues were quantified by HPLC. The relative expression of the genes involved in the proposed pathway was investigated by qRT-PCR, which indicated high expression levels in the bud tissue. The present results may lead to the design strategies to preserve the genetic diversity of endangered D. kotschyi plants and apply the new methods for engineering its valuable methoxylated-flavones pathway.https://doi.org/10.1038/s41598-021-99066-6 |
| spellingShingle | Abdonaser Poursalavati Sajad Rashidi-Monfared Amin Ebrahimi Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss Scientific Reports |
| title | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
| title_full | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
| title_fullStr | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
| title_full_unstemmed | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
| title_short | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
| title_sort | toward understanding of the methoxylated flavonoid biosynthesis pathway in dracocephalum kotschyi boiss |
| url | https://doi.org/10.1038/s41598-021-99066-6 |
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