Occurrence of class 1, 2, and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Babylon Province, Iraq
Background: Clinical management of bacterial infections has faced significant difficulties in recent years due to the advent and spread of multiple drug-resistant (MDR) bacteria. Worldwide, nosocomial infections are brought on by Pseudomonas aeruginosa, a clinically significant Pseudomonas species....
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Medical Journal of Babylon |
| Subjects: | |
| Online Access: | http://www.medjbabylon.org/article.asp?issn=1812-156X;year=2023;volume=20;issue=1;spage=181;epage=187;aulast=Almuttairi |
| _version_ | 1797775453786734592 |
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| author | Ahmed Abdulkareem Almuttairi Anwar A Abdulla |
| author_facet | Ahmed Abdulkareem Almuttairi Anwar A Abdulla |
| author_sort | Ahmed Abdulkareem Almuttairi |
| collection | DOAJ |
| description | Background: Clinical management of bacterial infections has faced significant difficulties in recent years due to the advent and spread of multiple drug-resistant (MDR) bacteria. Worldwide, nosocomial infections are brought on by Pseudomonas aeruginosa, a clinically significant Pseudomonas species. Objectives: This research aimed to identify class 1, 2, and 3 integrons in P. aeruginosa in Babylon, Iraq. Materials and Methods: From February 2022 to October 2022, 131 isolates from various sites including (burn, wound, and urine) were collected from different hospitals in Babylon Province for both genders and ages. These isolates were identified using traditional techniques as well as the Vitek 2 system (bioMerieux, France). Pseudomonas aeruginosa isolates were subjected to disc diffusion antimicrobial susceptibility testing. Class 1, 2, and 3 integron-specific primers were used in the polymerase chain reaction technique for the molecular identification of integron genes. Results: Pseudomonas aeruginosa isolates that were 131 (100%) had integron class 1. On the contrary, only five (3.81%) contained a class 2 integron. There was no presence of class 3 integron in any isolate. Conclusion: The MDR P. aeruginosa was highly prevalent (100%) and this suggested that the availability of class 1 integrons in our area was alarmingly high, showing the need for epidemiological monitoring. |
| first_indexed | 2024-03-12T22:35:57Z |
| format | Article |
| id | doaj.art-e7f6319bdd514900a27ca3b9214988fa |
| institution | Directory Open Access Journal |
| issn | 1812-156X 2312-6760 |
| language | English |
| last_indexed | 2024-03-12T22:35:57Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Babylon |
| spelling | doaj.art-e7f6319bdd514900a27ca3b9214988fa2023-07-21T12:46:31ZengWolters Kluwer Medknow PublicationsMedical Journal of Babylon1812-156X2312-67602023-01-0120118118710.4103/MJBL.MJBL_329_22Occurrence of class 1, 2, and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Babylon Province, IraqAhmed Abdulkareem AlmuttairiAnwar A AbdullaBackground: Clinical management of bacterial infections has faced significant difficulties in recent years due to the advent and spread of multiple drug-resistant (MDR) bacteria. Worldwide, nosocomial infections are brought on by Pseudomonas aeruginosa, a clinically significant Pseudomonas species. Objectives: This research aimed to identify class 1, 2, and 3 integrons in P. aeruginosa in Babylon, Iraq. Materials and Methods: From February 2022 to October 2022, 131 isolates from various sites including (burn, wound, and urine) were collected from different hospitals in Babylon Province for both genders and ages. These isolates were identified using traditional techniques as well as the Vitek 2 system (bioMerieux, France). Pseudomonas aeruginosa isolates were subjected to disc diffusion antimicrobial susceptibility testing. Class 1, 2, and 3 integron-specific primers were used in the polymerase chain reaction technique for the molecular identification of integron genes. Results: Pseudomonas aeruginosa isolates that were 131 (100%) had integron class 1. On the contrary, only five (3.81%) contained a class 2 integron. There was no presence of class 3 integron in any isolate. Conclusion: The MDR P. aeruginosa was highly prevalent (100%) and this suggested that the availability of class 1 integrons in our area was alarmingly high, showing the need for epidemiological monitoring.http://www.medjbabylon.org/article.asp?issn=1812-156X;year=2023;volume=20;issue=1;spage=181;epage=187;aulast=Almuttairiantibiotic resistanceintegronpseudomonas aeruginosa |
| spellingShingle | Ahmed Abdulkareem Almuttairi Anwar A Abdulla Occurrence of class 1, 2, and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Babylon Province, Iraq Medical Journal of Babylon antibiotic resistance integron pseudomonas aeruginosa |
| title | Occurrence of class 1, 2, and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Babylon Province, Iraq |
| title_full | Occurrence of class 1, 2, and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Babylon Province, Iraq |
| title_fullStr | Occurrence of class 1, 2, and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Babylon Province, Iraq |
| title_full_unstemmed | Occurrence of class 1, 2, and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Babylon Province, Iraq |
| title_short | Occurrence of class 1, 2, and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Babylon Province, Iraq |
| title_sort | occurrence of class 1 2 and 3 integrons among multidrug resistant pseudomonas aeruginosa in babylon province iraq |
| topic | antibiotic resistance integron pseudomonas aeruginosa |
| url | http://www.medjbabylon.org/article.asp?issn=1812-156X;year=2023;volume=20;issue=1;spage=181;epage=187;aulast=Almuttairi |
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