Attraction of <i>Frankliniella occidentalis</i> Females towards the Aggregation Pheromone Neryl (<i>S</i>)-2-Methylbutanoate and Kairomones in a Y-Olfactometer
An understanding of insect olfaction allows for more specific alternative methods of pest control. We evaluated the responses of the western flower thrips (WFT, <i>Frankliniella occidentalis</i>) in a Y-olfactometer to estimate gas-phase concentrations of the aggregation pheromone neryl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/6/562 |
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| author | Charles J. F. Chappuis Marilyn Cléroux Corentin Descombes Yannick Barth François Lefort |
| author_facet | Charles J. F. Chappuis Marilyn Cléroux Corentin Descombes Yannick Barth François Lefort |
| author_sort | Charles J. F. Chappuis |
| collection | DOAJ |
| description | An understanding of insect olfaction allows for more specific alternative methods of pest control. We evaluated the responses of the western flower thrips (WFT, <i>Frankliniella occidentalis</i>) in a Y-olfactometer to estimate gas-phase concentrations of the aggregation pheromone neryl (<i>S</i>)-2-methylbutanoate and known kairomones such as methyl isonicotinate, (<i>S</i>)-(-)-verbenone, and <i>p</i>-anisaldehyde. The gas-phase concentrations of these compounds were obtained from the release rates measured in dynamic headspace cells. The compounds were collected from the headspace using dried solid-phase extraction (SPE) cartridges and analyzed with a triple quadrupole GC-MS/MS. We observed that the aggregation pheromone significantly attracted WFT females at doses of 10 and 100 µg, whereas methyl isonicotinate and <i>p</i>-anisaldehyde significantly attracted WFT females at the highest dose. Verbenone did not produce any significant results. A completely different picture was obtained when the gas-phase concentrations were considered. The minimal gas-phase concentrations of the pheromone required to attract WFT females was 0.027 ng/mL, at least 100 times lower than that of the other two compounds. The relevance and implications of our results are discussed in light of the insect’s biology and pest management methods. |
| first_indexed | 2024-03-11T02:19:53Z |
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| id | doaj.art-e7f681bef8a24b25ab8dc33a2608f3c0 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T02:19:53Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-e7f681bef8a24b25ab8dc33a2608f3c02023-11-18T10:56:31ZengMDPI AGInsects2075-44502023-06-0114656210.3390/insects14060562Attraction of <i>Frankliniella occidentalis</i> Females towards the Aggregation Pheromone Neryl (<i>S</i>)-2-Methylbutanoate and Kairomones in a Y-OlfactometerCharles J. F. Chappuis0Marilyn Cléroux1Corentin Descombes2Yannick Barth3François Lefort4Analytical Chemistry Group, Haute École de Viticulture et Oenologie de Changins, HES-SO University of Applied Sciences and Arts Western Switzerland, 1260 Nyon, SwitzerlandAnalytical Chemistry Group, Haute École de Viticulture et Oenologie de Changins, HES-SO University of Applied Sciences and Arts Western Switzerland, 1260 Nyon, SwitzerlandPlants and Pathogens Group, Research Institute Land Nature Environment, Geneva School of Engineering Architecture and Landscape, HES-SO University of Applied Sciences and Arts Western Switzerland, 1254 Jussy, SwitzerlandPlants and Pathogens Group, Research Institute Land Nature Environment, Geneva School of Engineering Architecture and Landscape, HES-SO University of Applied Sciences and Arts Western Switzerland, 1254 Jussy, SwitzerlandPlants and Pathogens Group, Research Institute Land Nature Environment, Geneva School of Engineering Architecture and Landscape, HES-SO University of Applied Sciences and Arts Western Switzerland, 1254 Jussy, SwitzerlandAn understanding of insect olfaction allows for more specific alternative methods of pest control. We evaluated the responses of the western flower thrips (WFT, <i>Frankliniella occidentalis</i>) in a Y-olfactometer to estimate gas-phase concentrations of the aggregation pheromone neryl (<i>S</i>)-2-methylbutanoate and known kairomones such as methyl isonicotinate, (<i>S</i>)-(-)-verbenone, and <i>p</i>-anisaldehyde. The gas-phase concentrations of these compounds were obtained from the release rates measured in dynamic headspace cells. The compounds were collected from the headspace using dried solid-phase extraction (SPE) cartridges and analyzed with a triple quadrupole GC-MS/MS. We observed that the aggregation pheromone significantly attracted WFT females at doses of 10 and 100 µg, whereas methyl isonicotinate and <i>p</i>-anisaldehyde significantly attracted WFT females at the highest dose. Verbenone did not produce any significant results. A completely different picture was obtained when the gas-phase concentrations were considered. The minimal gas-phase concentrations of the pheromone required to attract WFT females was 0.027 ng/mL, at least 100 times lower than that of the other two compounds. The relevance and implications of our results are discussed in light of the insect’s biology and pest management methods.https://www.mdpi.com/2075-4450/14/6/562western flower thripspest managementolfactiongas-phase concentrationolfactometer |
| spellingShingle | Charles J. F. Chappuis Marilyn Cléroux Corentin Descombes Yannick Barth François Lefort Attraction of <i>Frankliniella occidentalis</i> Females towards the Aggregation Pheromone Neryl (<i>S</i>)-2-Methylbutanoate and Kairomones in a Y-Olfactometer Insects western flower thrips pest management olfaction gas-phase concentration olfactometer |
| title | Attraction of <i>Frankliniella occidentalis</i> Females towards the Aggregation Pheromone Neryl (<i>S</i>)-2-Methylbutanoate and Kairomones in a Y-Olfactometer |
| title_full | Attraction of <i>Frankliniella occidentalis</i> Females towards the Aggregation Pheromone Neryl (<i>S</i>)-2-Methylbutanoate and Kairomones in a Y-Olfactometer |
| title_fullStr | Attraction of <i>Frankliniella occidentalis</i> Females towards the Aggregation Pheromone Neryl (<i>S</i>)-2-Methylbutanoate and Kairomones in a Y-Olfactometer |
| title_full_unstemmed | Attraction of <i>Frankliniella occidentalis</i> Females towards the Aggregation Pheromone Neryl (<i>S</i>)-2-Methylbutanoate and Kairomones in a Y-Olfactometer |
| title_short | Attraction of <i>Frankliniella occidentalis</i> Females towards the Aggregation Pheromone Neryl (<i>S</i>)-2-Methylbutanoate and Kairomones in a Y-Olfactometer |
| title_sort | attraction of i frankliniella occidentalis i females towards the aggregation pheromone neryl i s i 2 methylbutanoate and kairomones in a y olfactometer |
| topic | western flower thrips pest management olfaction gas-phase concentration olfactometer |
| url | https://www.mdpi.com/2075-4450/14/6/562 |
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