Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters
Proteobacteria produce N-acylhomoserine lactones as signaling molecules, which will bind to their cognate receptor and activate quorum sensing-mediated phenotypes in a population-dependent manner. Although quorum sensing signaling molecules can be degraded by bacteria or fungi, there is no reported...
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| Format: | Article |
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MDPI AG
2013-09-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/13/10/12943 |
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| author | Cheng-Siang Wong Chong-Lek Koh Choon-Kook Sam Jian Woon Chen Yee Meng Chong Wai-Fong Yin Kok-Gan Chan |
| author_facet | Cheng-Siang Wong Chong-Lek Koh Choon-Kook Sam Jian Woon Chen Yee Meng Chong Wai-Fong Yin Kok-Gan Chan |
| author_sort | Cheng-Siang Wong |
| collection | DOAJ |
| description | Proteobacteria produce N-acylhomoserine lactones as signaling molecules, which will bind to their cognate receptor and activate quorum sensing-mediated phenotypes in a population-dependent manner. Although quorum sensing signaling molecules can be degraded by bacteria or fungi, there is no reported work on the degradation of such molecules by basidiomycetous yeast. By using a minimal growth medium containing N-3-oxohexanoylhomoserine lactone as the sole source of carbon, a wetland water sample from Malaysia was enriched for microbial strains that can degrade N-acylhomoserine lactones, and consequently, a basidiomycetous yeast strain WW1C was isolated. Morphological phenotype and molecular analyses confirmed that WW1C was a strain of Trichosporon loubieri. We showed that WW1C degraded AHLs with N-acyl side chains ranging from 4 to 10 carbons in length, with or without oxo group substitutions at the C3 position. Re-lactonisation bioassays revealed that WW1C degraded AHLs via a lactonase activity. To the best of our knowledge, this is the first report of degradation of N-acyl-homoserine lactones and utilization of N-3-oxohexanoylhomoserine as carbon and nitrogen source for growth by basidiomycetous yeast from tropical wetland water; and the degradation of bacterial quorum sensing molecules by an eukaryotic yeast. |
| first_indexed | 2024-04-11T12:18:47Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:18:47Z |
| publishDate | 2013-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-e7f7abe42c66409f9ba842134bfb8e2e2022-12-22T04:24:11ZengMDPI AGSensors1424-82202013-09-011310129431295710.3390/s131012943Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland WatersCheng-Siang WongChong-Lek KohChoon-Kook SamJian Woon ChenYee Meng ChongWai-Fong YinKok-Gan ChanProteobacteria produce N-acylhomoserine lactones as signaling molecules, which will bind to their cognate receptor and activate quorum sensing-mediated phenotypes in a population-dependent manner. Although quorum sensing signaling molecules can be degraded by bacteria or fungi, there is no reported work on the degradation of such molecules by basidiomycetous yeast. By using a minimal growth medium containing N-3-oxohexanoylhomoserine lactone as the sole source of carbon, a wetland water sample from Malaysia was enriched for microbial strains that can degrade N-acylhomoserine lactones, and consequently, a basidiomycetous yeast strain WW1C was isolated. Morphological phenotype and molecular analyses confirmed that WW1C was a strain of Trichosporon loubieri. We showed that WW1C degraded AHLs with N-acyl side chains ranging from 4 to 10 carbons in length, with or without oxo group substitutions at the C3 position. Re-lactonisation bioassays revealed that WW1C degraded AHLs via a lactonase activity. To the best of our knowledge, this is the first report of degradation of N-acyl-homoserine lactones and utilization of N-3-oxohexanoylhomoserine as carbon and nitrogen source for growth by basidiomycetous yeast from tropical wetland water; and the degradation of bacterial quorum sensing molecules by an eukaryotic yeast.http://www.mdpi.com/1424-8220/13/10/12943basidiomycetousbiosensorlactonaseN-acylhomoserine lactonequorum sensingquorum quenchingRapid Resolution Liquid ChromatographyTrichosporon loubieriyeast |
| spellingShingle | Cheng-Siang Wong Chong-Lek Koh Choon-Kook Sam Jian Woon Chen Yee Meng Chong Wai-Fong Yin Kok-Gan Chan Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters Sensors basidiomycetous biosensor lactonase N-acylhomoserine lactone quorum sensing quorum quenching Rapid Resolution Liquid Chromatography Trichosporon loubieri yeast |
| title | Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters |
| title_full | Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters |
| title_fullStr | Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters |
| title_full_unstemmed | Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters |
| title_short | Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters |
| title_sort | degradation of bacterial quorum sensing signaling molecules by the microscopic yeast trichosporon loubieri isolated from tropical wetland waters |
| topic | basidiomycetous biosensor lactonase N-acylhomoserine lactone quorum sensing quorum quenching Rapid Resolution Liquid Chromatography Trichosporon loubieri yeast |
| url | http://www.mdpi.com/1424-8220/13/10/12943 |
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