Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress
The Arabian camel is the largest known mammal that can survive in severe hot climatic conditions. We provide the molecular explanation for the thermotolerance of camel granulosa somatic cells after exposure to 45 °C for 2 (acute heat shock) or 20 h (chronic heat shock). The common features of the ce...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-03-01
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| Series: | Journal of Advanced Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090123219301870 |
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| author | Islam M. Saadeldin Ayman Abdel-Aziz Swelum Mona Elsafadi Amer Mahmood Aya Osama Hassan Shikshaky Musaad Alfayez Abdullah N. Alowaimer Sameh Magdeldin |
| author_facet | Islam M. Saadeldin Ayman Abdel-Aziz Swelum Mona Elsafadi Amer Mahmood Aya Osama Hassan Shikshaky Musaad Alfayez Abdullah N. Alowaimer Sameh Magdeldin |
| author_sort | Islam M. Saadeldin |
| collection | DOAJ |
| description | The Arabian camel is the largest known mammal that can survive in severe hot climatic conditions. We provide the molecular explanation for the thermotolerance of camel granulosa somatic cells after exposure to 45 °C for 2 (acute heat shock) or 20 h (chronic heat shock). The common features of the cellular responses to acute heat stress were the increase of heat shock proteins and DNA repair enzymes expression. Actin polymerization and Rho signaling were critically activated as a cellular defense against heat shock. Cells exposed to chronic heat shock showed altered cell architecture with a decrease in total detected proteins, metabolic enzymes, and cytoskeletal protein expression. Treatment with transforming growth factor beta (TGFβ) pathway inhibitor SB-431542 suppressed the morphological alterations of cells exposed to chronic heat shock. Moreover, during the recovery stage at 38 °C for 24 h, proteomic changes were partially restored with an exponential increase in HSP70 expression, and the cells restored their normal cellular morphology on the 9th day of recovery. Full proteomics data are available via ProteomeXchange with identifier PXD012159. The strategies of cellular defense and tolerance to both thermal conditions reflect the flexible adaptability of camel somatic cells to conserve life under extremely hot conditions. Keywords: HSPs, ROCK, Actin, TGFβ, Proteomics, Camel, Anastasis |
| first_indexed | 2024-12-11T02:29:50Z |
| format | Article |
| id | doaj.art-e7facf3ba12648d8ad5ed161c68361b8 |
| institution | Directory Open Access Journal |
| issn | 2090-1232 |
| language | English |
| last_indexed | 2024-12-11T02:29:50Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-e7facf3ba12648d8ad5ed161c68361b82022-12-22T01:23:51ZengElsevierJournal of Advanced Research2090-12322020-03-0122105118Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stressIslam M. Saadeldin0Ayman Abdel-Aziz Swelum1Mona Elsafadi2Amer Mahmood3Aya Osama4Hassan Shikshaky5Musaad Alfayez6Abdullah N. Alowaimer7Sameh Magdeldin8Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, 11451 Riyadh, Saudi Arabia; Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44519 Zagazig, Egypt; Corresponding authors at: Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, 11451 Riyadh, Saudi Arabia (I.M. Saadeldin).Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, 11451 Riyadh, Saudi Arabia; Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, 44519 Zagazig, EgyptStem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh, Saudi ArabiaStem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh, Saudi ArabiaProteomics and Metabolomics Unit, 57357 Children’s Cancer Hospital, Cairo, EgyptProteomics and Metabolomics Unit, 57357 Children’s Cancer Hospital, Cairo, EgyptStem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh, Saudi Arabia; Saudi Society for Camel Research, King Saud University, Saudi ArabiaDepartment of Animal Production, College of Food and Agricultural Sciences, King Saud University, 11451 Riyadh, Saudi ArabiaProteomics and Metabolomics Unit, 57357 Children’s Cancer Hospital, Cairo, Egypt; Physiology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt; Corresponding authors at: Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, 11451 Riyadh, Saudi Arabia (I.M. Saadeldin).The Arabian camel is the largest known mammal that can survive in severe hot climatic conditions. We provide the molecular explanation for the thermotolerance of camel granulosa somatic cells after exposure to 45 °C for 2 (acute heat shock) or 20 h (chronic heat shock). The common features of the cellular responses to acute heat stress were the increase of heat shock proteins and DNA repair enzymes expression. Actin polymerization and Rho signaling were critically activated as a cellular defense against heat shock. Cells exposed to chronic heat shock showed altered cell architecture with a decrease in total detected proteins, metabolic enzymes, and cytoskeletal protein expression. Treatment with transforming growth factor beta (TGFβ) pathway inhibitor SB-431542 suppressed the morphological alterations of cells exposed to chronic heat shock. Moreover, during the recovery stage at 38 °C for 24 h, proteomic changes were partially restored with an exponential increase in HSP70 expression, and the cells restored their normal cellular morphology on the 9th day of recovery. Full proteomics data are available via ProteomeXchange with identifier PXD012159. The strategies of cellular defense and tolerance to both thermal conditions reflect the flexible adaptability of camel somatic cells to conserve life under extremely hot conditions. Keywords: HSPs, ROCK, Actin, TGFβ, Proteomics, Camel, Anastasishttp://www.sciencedirect.com/science/article/pii/S2090123219301870 |
| spellingShingle | Islam M. Saadeldin Ayman Abdel-Aziz Swelum Mona Elsafadi Amer Mahmood Aya Osama Hassan Shikshaky Musaad Alfayez Abdullah N. Alowaimer Sameh Magdeldin Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress Journal of Advanced Research |
| title | Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress |
| title_full | Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress |
| title_fullStr | Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress |
| title_full_unstemmed | Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress |
| title_short | Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress |
| title_sort | thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress |
| url | http://www.sciencedirect.com/science/article/pii/S2090123219301870 |
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