Subtype Characterization and Zoonotic Potential of <i>Cryptosporidium felis</i> in Cats in Guangdong and Shanghai, China
<i>Cryptosporidium</i><i>felis</i> is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present stu...
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MDPI AG
2021-01-01
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| Series: | Pathogens |
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| author | Jiayu Li Fuxian Yang Ruobing Liang Sheng Guo Yaqiong Guo Na Li Yaoyu Feng Lihua Xiao |
| author_facet | Jiayu Li Fuxian Yang Ruobing Liang Sheng Guo Yaqiong Guo Na Li Yaoyu Feng Lihua Xiao |
| author_sort | Jiayu Li |
| collection | DOAJ |
| description | <i>Cryptosporidium</i><i>felis</i> is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of <i>C. felis</i> in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (<i>gp60</i>) gene was employed to identify the subtypes of 30 cat-derived <i>C. felis</i> isolates from Guangdong and Shanghai. Altogether, 20 <i>C. felis</i> isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the <i>gp60</i> sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of <i>C. felis</i>. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of <i>C. felis</i>. |
| first_indexed | 2024-03-09T04:14:32Z |
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| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T04:14:32Z |
| publishDate | 2021-01-01 |
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| spelling | doaj.art-e7fb609bba614af58da8904b73b253d42023-12-03T13:56:46ZengMDPI AGPathogens2076-08172021-01-011028910.3390/pathogens10020089Subtype Characterization and Zoonotic Potential of <i>Cryptosporidium felis</i> in Cats in Guangdong and Shanghai, ChinaJiayu Li0Fuxian Yang1Ruobing Liang2Sheng Guo3Yaqiong Guo4Na Li5Yaoyu Feng6Lihua Xiao7Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, ChinaCenter for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, ChinaCenter for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, ChinaCenter for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, ChinaCenter for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, ChinaCenter for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, ChinaCenter for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, ChinaCenter for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, China<i>Cryptosporidium</i><i>felis</i> is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of <i>C. felis</i> in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (<i>gp60</i>) gene was employed to identify the subtypes of 30 cat-derived <i>C. felis</i> isolates from Guangdong and Shanghai. Altogether, 20 <i>C. felis</i> isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the <i>gp60</i> sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of <i>C. felis</i>. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of <i>C. felis</i>.https://www.mdpi.com/2076-0817/10/2/89<i>Cryptosporidium felis</i>60-kDa glycoproteinsubtypeszoonotic transmission |
| spellingShingle | Jiayu Li Fuxian Yang Ruobing Liang Sheng Guo Yaqiong Guo Na Li Yaoyu Feng Lihua Xiao Subtype Characterization and Zoonotic Potential of <i>Cryptosporidium felis</i> in Cats in Guangdong and Shanghai, China Pathogens <i>Cryptosporidium felis</i> 60-kDa glycoprotein subtypes zoonotic transmission |
| title | Subtype Characterization and Zoonotic Potential of <i>Cryptosporidium felis</i> in Cats in Guangdong and Shanghai, China |
| title_full | Subtype Characterization and Zoonotic Potential of <i>Cryptosporidium felis</i> in Cats in Guangdong and Shanghai, China |
| title_fullStr | Subtype Characterization and Zoonotic Potential of <i>Cryptosporidium felis</i> in Cats in Guangdong and Shanghai, China |
| title_full_unstemmed | Subtype Characterization and Zoonotic Potential of <i>Cryptosporidium felis</i> in Cats in Guangdong and Shanghai, China |
| title_short | Subtype Characterization and Zoonotic Potential of <i>Cryptosporidium felis</i> in Cats in Guangdong and Shanghai, China |
| title_sort | subtype characterization and zoonotic potential of i cryptosporidium felis i in cats in guangdong and shanghai china |
| topic | <i>Cryptosporidium felis</i> 60-kDa glycoprotein subtypes zoonotic transmission |
| url | https://www.mdpi.com/2076-0817/10/2/89 |
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