Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma
In humans, deviation from a core airway microbiota may predispose to development, exacerbation, or progression of asthma. We proposed to describe microbiota changes using 16 rRNA sequencing in samples from the upper and lower airways, and rectal swabs of 8 cats after experimental induction of asthma...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.983375/full |
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| author | Aida I. Vientós-Plotts Aida I. Vientós-Plotts Aida I. Vientós-Plotts Aaron C. Ericsson Aaron C. Ericsson Aaron C. Ericsson Zachary L. McAdams Hansjorg Rindt Hansjorg Rindt Carol R. Reinero Carol R. Reinero Carol R. Reinero |
| author_facet | Aida I. Vientós-Plotts Aida I. Vientós-Plotts Aida I. Vientós-Plotts Aaron C. Ericsson Aaron C. Ericsson Aaron C. Ericsson Zachary L. McAdams Hansjorg Rindt Hansjorg Rindt Carol R. Reinero Carol R. Reinero Carol R. Reinero |
| author_sort | Aida I. Vientós-Plotts |
| collection | DOAJ |
| description | In humans, deviation from a core airway microbiota may predispose to development, exacerbation, or progression of asthma. We proposed to describe microbiota changes using 16 rRNA sequencing in samples from the upper and lower airways, and rectal swabs of 8 cats after experimental induction of asthma using Bermuda grass allergen, in acute (6 weeks) and chronic (36 weeks) stages. We hypothesized that asthma induction would decrease richness and diversity and alter microbiota composition and structure in the lower airways, without significantly impacting other sites. After asthma induction, richness decreased in rectal (p = 0.014) and lower airway (p = 0.016) samples. B diversity was significantly different between health and chronic asthma in all sites, and between all time points for lower airways. In healthy lower airways Pseudomonadaceae comprised 80.4 ± 1.3% whereas Sphingobacteriaceae and Xanthobacteraceae predominated (52.4 ± 2.2% and 33.5 ± 2.1%, respectively), and Pseudomonadaceae was absent, in 6/8 cats with chronic asthma. This study provides evidence that experimental induction of asthma leads to dysbiosis in the airways and distant sites in both the acute and chronic stages of disease.
This article has been published alongside “Respiratory dysbiosis in cats with spontaneous allergic asthma” (1). |
| first_indexed | 2024-04-13T03:01:28Z |
| format | Article |
| id | doaj.art-e7fff3eccbe1439d98daac17c1e8a662 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-13T03:01:28Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-e7fff3eccbe1439d98daac17c1e8a6622022-12-22T03:05:26ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-08-01910.3389/fvets.2022.983375983375Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthmaAida I. Vientós-Plotts0Aida I. Vientós-Plotts1Aida I. Vientós-Plotts2Aaron C. Ericsson3Aaron C. Ericsson4Aaron C. Ericsson5Zachary L. McAdams6Hansjorg Rindt7Hansjorg Rindt8Carol R. Reinero9Carol R. Reinero10Carol R. Reinero11College of Veterinary Medicine, University of Missouri, Columbia, MO, United StatesDepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO, United StatesComparative Internal Medicine Laboratory, University of Missouri, Columbia, MO, United StatesCollege of Veterinary Medicine, University of Missouri, Columbia, MO, United StatesUniversity of Missouri Metagenomics Center, University of Missouri, Columbia, MO, United StatesDepartment of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO, United StatesDepartment of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO, United StatesCollege of Veterinary Medicine, University of Missouri, Columbia, MO, United StatesComparative Internal Medicine Laboratory, University of Missouri, Columbia, MO, United StatesCollege of Veterinary Medicine, University of Missouri, Columbia, MO, United StatesDepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO, United StatesComparative Internal Medicine Laboratory, University of Missouri, Columbia, MO, United StatesIn humans, deviation from a core airway microbiota may predispose to development, exacerbation, or progression of asthma. We proposed to describe microbiota changes using 16 rRNA sequencing in samples from the upper and lower airways, and rectal swabs of 8 cats after experimental induction of asthma using Bermuda grass allergen, in acute (6 weeks) and chronic (36 weeks) stages. We hypothesized that asthma induction would decrease richness and diversity and alter microbiota composition and structure in the lower airways, without significantly impacting other sites. After asthma induction, richness decreased in rectal (p = 0.014) and lower airway (p = 0.016) samples. B diversity was significantly different between health and chronic asthma in all sites, and between all time points for lower airways. In healthy lower airways Pseudomonadaceae comprised 80.4 ± 1.3% whereas Sphingobacteriaceae and Xanthobacteraceae predominated (52.4 ± 2.2% and 33.5 ± 2.1%, respectively), and Pseudomonadaceae was absent, in 6/8 cats with chronic asthma. This study provides evidence that experimental induction of asthma leads to dysbiosis in the airways and distant sites in both the acute and chronic stages of disease. This article has been published alongside “Respiratory dysbiosis in cats with spontaneous allergic asthma” (1).https://www.frontiersin.org/articles/10.3389/fvets.2022.983375/fullrespiratory microbiotainflammatory airway diseaselarge animal model16S rRNA genetranslational researchgut-lung axis |
| spellingShingle | Aida I. Vientós-Plotts Aida I. Vientós-Plotts Aida I. Vientós-Plotts Aaron C. Ericsson Aaron C. Ericsson Aaron C. Ericsson Zachary L. McAdams Hansjorg Rindt Hansjorg Rindt Carol R. Reinero Carol R. Reinero Carol R. Reinero Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma Frontiers in Veterinary Science respiratory microbiota inflammatory airway disease large animal model 16S rRNA gene translational research gut-lung axis |
| title | Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma |
| title_full | Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma |
| title_fullStr | Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma |
| title_full_unstemmed | Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma |
| title_short | Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma |
| title_sort | temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma |
| topic | respiratory microbiota inflammatory airway disease large animal model 16S rRNA gene translational research gut-lung axis |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.983375/full |
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